FastQCFastQC Report
Thu 26 May 2016
SRR1926653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660168
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14980.22691193756740707No Hit
CTGTAGGACGTGGAATATGGCAAGA14130.21403642709128587No Hit
GTCCTACAGTGGACATTTCTAAATT13530.20494783146108264No Hit
CTTTAGGACGTGAAATATGGCGAGG13050.19767695495692006No Hit
GTCCTAAAGTGTGTATTTCTCATTT12870.19495037626785908No Hit
GTCCTACAGTGTGCATTTCTCATTT9940.15056773427370002No Hit
GGTATCAACGCAGAGTACTTTTTTT9590.14526605348941482No Hit
CTGTAGGACCTGGAATATGGCGAGA8730.13223906641945685No Hit
TATCAACGCAGAGTACTTTTTTTTT8060.1220901346323966No Hit
CCCATGTACTCTGCGTTGATACCAC7970.12072684528786613No Hit
GTACATGGGAAGCAGTGGTATCAAC7660.11603107087892778No Hit
CTGAAGGACCTGGAATATGGCGAGA7150.10830576459325504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC851.6259946E-814.5853783
CCAACGA602.629714E-514.21171419
GGCGAGG3600.013.94853419
CCCCTAG551.8933992E-413.8724671
GGTCTAC706.959588E-613.6247441
ATAGACC500.001455159213.3512313
TAGGACC3450.013.2642594
TCCAACG802.0555945E-613.02740418
GTGCTAG603.9534643E-412.7164281
ACTGGCC751.4691648E-512.6719858
GTCCTAT701.0499045E-412.262271
CTAGACT550.00298512.1337834
CTGTAGG9250.011.9603161
CCAGTAC802.7500811E-511.9207423
TGGCGAG9250.011.67665118
TTAGAAC907.1302657E-611.6558373
TGTAGGA9800.011.5836822
CCTAGAC751.9977042E-411.4439123
TGCGTTG2000.011.37626312
GTAGGAC9400.011.3627493
Produced by FastQC (version 0.11.5)