FastQCFastQC Report
Thu 26 May 2016
SRR1926652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715328
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12420.17362664400107364No Hit
GTCCTAAAGTGTGTATTTCTCATTT10650.1488827502907757No Hit
CTGTAGGACGTGGAATATGGCAAGA8670.12120314037756108No Hit
CTTTAGGACGTGAAATATGGCGAGG8450.11812762816498165No Hit
GTCCTACAGTGTGCATTTCTCATTT8330.11645007604902927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA806.247119E-717.1736642
GTCCTAG808.1902544E-716.735061
TCTTAGG500.001995849716.4867172
CTCTACA801.1869943E-515.4562972
TCTAGGA1003.8391954E-715.1128242
TAGGACC3300.014.6421764
CCTATAC450.004407173514.6399593
TGTAGGA7900.014.6084832
GTCCTAA4400.014.60514451
CCCCTAT658.1326475E-414.4178991
GTCTTAG658.1326475E-414.4178991
GTCCTAC8000.014.2248021
TTTAGGA5650.014.10368252
CTGTAGG7950.013.9774591
GTATTAG700.001423603713.3880491
CTTTAGG5600.013.3880491
TTAGGAC5750.013.1759633
TCTACAC1107.523522E-812.9763283
TAGAGTG1057.716335E-812.9516075
AGGACGT8450.012.87496955
Produced by FastQC (version 0.11.5)