FastQCFastQC Report
Thu 26 May 2016
SRR1926652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715328
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA25470.35606043661089737No Hit
GTCCTACAGTGGACATTTCTAAATT24090.33676858727744474No Hit
CTTTAGGACGTGAAATATGGCGAGG23760.33215531895857564No Hit
GTCCTAAAGTGTGTATTTCTCATTT22300.3117451015478214No Hit
GTCCTACAGTGTGCATTTCTCATTT16820.2351368882526617No Hit
GTATCAACGCAGAGTACTTTTTTTT15790.22073789925740359No Hit
CTGTAGGACCTGGAATATGGCGAGA15630.21850116310280038No Hit
CTGAAGGACCTGGAATATGGCGAGA12640.17670215621365304No Hit
GTACATGGGAAGCAGTGGTATCAAC9740.13616131341146998No Hit
GGTATCAACGCAGAGTACTTTTTTT9510.1329460051892279No Hit
CCCATGTACTCTGCGTTGATACCAC9240.12917151292833498No Hit
GATATACACTGTTCTACAAATCCCG8300.11603068802004116No Hit
GAATATGGCAAGAAAACTGAAAATC7850.10973986758521966No Hit
TATCAACGCAGAGTACTTTTTTTTT7620.10652455936297754No Hit
GTCCTTCAGTGTGCATTTCTCATTT7450.10414802719871165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAT400.00534148314.22328810
AAAGGCG551.8921736E-413.8738265
AAGGCGT551.8921736E-413.8738266
TAGGACC5650.013.8431324
CCAACGA905.585953E-712.62966519
TGTAGGA14800.012.5054892
AGGACTA701.04918145E-412.2634715
GGACCTG10100.012.1825256
CTGTAGG14100.012.1054411
TCCAACG1103.9584847E-812.05559118
AGGACCT10550.012.0245315
GACGTGG6900.011.9826387
CTATAGG1459.094947E-1111.837261
GGTCTAC907.141136E-611.6545871
CTCTAGG1406.366463E-1011.5789081
GGCGAGG5900.011.55935519
GTAGGAC14700.011.4864563
AATCCCG2150.011.45481319
GGACGTG13750.011.4459086
CTACTAT752.0001437E-411.4426851
Produced by FastQC (version 0.11.5)