##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715328 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.213365057707794 31.0 28.0 31.0 20.0 34.0 2 28.64618748322448 31.0 28.0 33.0 22.0 34.0 3 29.01057277221079 31.0 28.0 33.0 23.0 34.0 4 32.73210331484298 35.0 33.0 37.0 26.0 37.0 5 32.37171758969312 35.0 33.0 37.0 25.0 37.0 6 32.41737916032925 35.0 33.0 37.0 25.0 37.0 7 32.21263672049745 35.0 33.0 37.0 21.0 37.0 8 32.17607307417017 35.0 33.0 37.0 21.0 37.0 9 33.36991841504876 37.0 33.0 39.0 18.0 39.0 10 33.259879384002865 37.0 33.0 39.0 18.0 39.0 11 33.50345016551847 37.0 33.0 39.0 22.0 39.0 12 33.30751207837523 37.0 33.0 39.0 18.0 39.0 13 33.432047955623155 37.0 33.0 39.0 21.0 39.0 14 34.03104729578599 38.0 33.0 40.0 17.0 41.0 15 34.16979763129641 38.0 33.0 40.0 18.0 41.0 16 34.15436415182965 38.0 33.0 40.0 18.0 41.0 17 34.08572011720497 38.0 33.0 40.0 17.0 41.0 18 34.06261463272792 38.0 33.0 40.0 17.0 41.0 19 34.087605965375325 39.0 34.0 40.0 18.0 41.0 20 34.07151824058334 39.0 34.0 40.0 18.0 41.0 21 33.98345374429632 39.0 34.0 40.0 17.0 41.0 22 33.91968998836897 39.0 34.0 40.0 11.0 41.0 23 33.558626252572246 39.0 33.0 40.0 10.0 41.0 24 33.64534171736602 39.0 33.0 40.0 2.0 41.0 25 33.42670075825356 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6853673798786986 1101 2 0.7740476883688245 1101 3 0.6852941851596057 1101 4 0.6282522900547249 1101 5 0.42367847163941974 1101 6 0.1516964134101002 1101 7 0.4003760271722143 1101 8 0.32101995463487043 1101 9 0.6130644088663857 1101 10 0.8148673084510278 1101 11 0.5461792216962422 1101 12 0.5499507705194162 1101 13 0.468804728827358 1101 14 0.5534084781611455 1101 15 0.7841513368663797 1101 16 0.5595934949833108 1101 17 0.6559304387348561 1101 18 0.5844061520747346 1101 19 0.5875418323129082 1101 20 0.5005684408937441 1101 21 0.5928812202651486 1101 22 0.7238086301728757 1101 23 0.9793493772147883 1101 24 0.9365066030313187 1101 25 0.9455150551909242 1102 1 0.47320778095377136 1102 2 0.5935022423537717 1102 3 0.30315288394444906 1102 4 0.209424415873265 1102 5 0.3851681599081047 1102 6 0.06961141284039485 1102 7 0.44065578776042 1102 8 0.5254207884206181 1102 9 0.688507273764948 1102 10 0.5851707287152408 1102 11 0.4513086126438992 1102 12 0.6454493998667985 1102 13 0.3392261123486975 1102 14 0.6723523048624003 1102 15 0.3203097561587356 1102 16 0.5080375194070683 1102 17 0.39216861727492613 1102 18 0.6890348694253845 1102 19 0.6239958299938593 1102 20 0.7115035007802035 1102 21 0.6877692557077424 1102 22 0.7831180089220666 1102 23 1.1918233214101193 1102 24 0.9411473848060012 1102 25 1.0423870181397348 1103 1 -0.2552594122813403 1103 2 -0.18045759112380466 1103 3 -0.24218616433268636 1103 4 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0.03919694089196213 2204 5 0.25472219101700944 2204 6 0.2062581428022341 2204 7 0.38668814691592956 2204 8 0.4484858998241279 2204 9 0.9315913027446001 2204 10 0.8944990319205814 2204 11 0.8148918690876172 2204 12 0.9171827739652727 2204 13 0.611094611991092 2204 14 0.8909199195007247 2204 15 0.7523497969966755 2204 16 0.6941463405117645 2204 17 0.6197623452658405 2204 18 0.7239231929405108 2204 19 0.5808614360387523 2204 20 0.6886290665331174 2204 21 0.8035445487527397 2204 22 0.7582228335864372 2204 23 1.1082488549512846 2204 24 0.8138850556237927 2204 25 0.8038187756453468 2205 1 0.6277521167276703 2205 2 0.6290673284528658 2205 3 0.6637475122289906 2205 4 0.5691271546621195 2205 5 0.6960988346873478 2205 6 0.5514275856817008 2205 7 0.3318862909104823 2205 8 0.4538208524672882 2205 9 0.7850880552665771 2205 10 0.6066693677236259 2205 11 0.5301396022310243 2205 12 0.7055811454427428 2205 13 0.6887018956962976 2205 14 1.0062239918700584 2205 15 1.0758724868226608 2205 16 0.9272924079274958 2205 17 0.9782366152476669 2205 18 0.9316049932445267 2205 19 1.0372397273657015 2205 20 0.9569074248293319 2205 21 1.0955916823617429 2205 22 0.900039561021515 2205 23 1.2326921064687255 2205 24 1.267822423412639 2205 25 1.368275367866282 2206 1 0.3372443568478225 2206 2 0.5811700055727052 2206 3 0.5730624647716596 2206 4 0.4071219211947721 2206 5 0.43403321505823556 2206 6 0.2273048624206382 2206 7 0.16862688331421083 2206 8 0.5286455003437069 2206 9 0.6396309526668773 2206 10 0.590198655012081 2206 11 0.34914369507087883 2206 12 0.43534145883144504 2206 13 0.2771792354020093 2206 14 0.593747797953057 2206 15 0.507763863988437 2206 16 0.4385193308654607 2206 17 0.558578597049241 2206 18 0.6395979017414248 2206 19 0.4488023870406437 2206 20 0.3836259788646217 2206 21 0.581892539638794 2206 22 0.42689204846686835 2206 23 0.9017423921417205 2206 24 0.6750251505962197 2206 25 0.7873875880641634 2207 1 -1.2586687950107383 2207 2 -0.38172943617766464 2207 3 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0.5356648998142006 2208 15 0.41551789426771535 2208 16 0.5090666138602842 2208 17 0.2485003963250918 2208 18 0.6773279810652326 2208 19 0.14777110430950557 2208 20 0.25816423325257887 2208 21 0.3138768311324185 2208 22 0.4400095302228806 2208 23 0.9714174550709629 2208 24 0.8605431235787577 2208 25 0.8665032582392413 2209 1 -0.18943995240943323 2209 2 -0.13127953251933278 2209 3 0.34649471172955515 2209 4 0.41368098811713594 2209 5 0.35591831234538773 2209 6 0.2196345215950899 2209 7 0.4772850086298064 2209 8 0.4897816552705905 2209 9 0.6886166127204021 2209 10 0.4560092243453724 2209 11 0.4021080950614575 2209 12 0.5025588132800678 2209 13 0.6680016434978882 2209 14 0.7368803999430185 2209 15 0.6815026106232196 2209 16 0.6487842575174625 2209 17 0.7549136824322247 2209 18 0.7898775843317765 2209 19 0.6164615039343602 2209 20 0.7727105089376991 2209 21 0.8178416944427127 2209 22 0.7478330534927835 2209 23 0.8568074203062039 2209 24 0.8880515830864937 2209 25 1.1084646407486147 2210 1 -0.18564942592694322 2210 2 -0.49909985350927855 2210 3 0.25608864142631305 2210 4 0.44628170021459823 2210 5 0.8217196131246567 2210 6 0.6314325950001063 2210 7 0.8523921782062089 2210 8 0.5957757793743284 2210 9 1.0127615769790097 2210 10 1.0620231441033496 2210 11 0.54583546658084 2210 12 0.5337601774054264 2210 13 0.43767579337693263 2210 14 0.9363830511954703 2210 15 0.607221124611236 2210 16 0.8359248640016972 2210 17 0.8419333306429735 2210 18 0.790720606158871 2210 19 0.8497956108909648 2210 20 0.925550707494935 2210 21 1.105552415769047 2210 22 1.0484635109498726 2210 23 1.1513674379994185 2210 24 0.906350276300266 2210 25 0.8474207135092513 2211 1 -1.161618606428533 2211 2 -0.4118570506127739 2211 3 0.3757680833196382 2211 4 0.3265139503029104 2211 5 0.6153879282076673 2211 6 0.515958822481565 2211 7 0.630882552632201 2211 8 0.42769250491300426 2211 9 0.8794716617547067 2211 10 0.849807937462586 2211 11 0.5133767505794253 2211 12 0.7584820071475704 2211 13 0.6985597566407975 2211 14 0.9364823977161336 2211 15 0.6409161528698064 2211 16 0.7977647615643946 2211 17 0.6272344609856049 2211 18 0.9218580134354539 2211 19 1.1299693569171012 2211 20 0.9080326043511704 2211 21 1.0276426665638212 2211 22 1.0045357910629065 2211 23 1.06038810207572 2211 24 1.1489103257527589 2211 25 1.2018559837317895 2212 1 0.9216179776787925 2212 2 -0.037131173419791 2212 3 0.34435683460962707 2212 4 0.49324511645357916 2212 5 0.5799992113174 2212 6 0.2521666828186824 2212 7 0.5769502340907522 2212 8 0.6400140563405969 2212 9 0.730811101039194 2212 10 0.4478579030628609 2212 11 0.4037115029014089 2212 12 0.5830649104843459 2212 13 0.33471816737350935 2212 14 0.3981159546476789 2212 15 0.527671183829554 2212 16 0.003786863387581718 2212 17 0.401765764735174 2212 18 0.345146627126546 2212 19 0.4088275010519524 2212 20 0.5306138737010144 2212 21 0.4734845998283532 2212 22 0.722302173931844 2212 23 1.1716497119828375 2212 24 0.8431182720891854 2212 25 0.2058012021700435 2213 1 0.28539048490548424 2213 2 0.16542951337713063 2213 3 -0.39374089115918665 2213 4 -1.1920368115929492 2213 5 -1.7484640863758614 2213 6 -0.5564943185860258 2213 7 -0.27351529255803797 2213 8 -0.1732557749785535 2213 9 -0.5372193497438431 2213 10 -0.4949061178800349 2213 11 -0.388629945223137 2213 12 -0.6875717929833058 2213 13 -0.3690701863338717 2213 14 -7.466443072041741 2213 15 -3.349196437108695 2213 16 -1.8427097873745595 2213 17 -1.0859503104456323 2213 18 -5.003039153994667 2213 19 -2.6829761535063774 2213 20 -5.077486115850757 2213 21 -2.079811526200505 2213 22 -1.6805380029914971 2213 23 -4.361523415172108 2213 24 -2.2351868510257766 2213 25 -1.5895078332262393 2214 1 0.24211534735837148 2214 2 0.05925094040134482 2214 3 -1.6472261400436494 2214 4 -2.235324945717629 2214 5 -0.3410369755150562 2214 6 -0.5959458630126591 2214 7 -0.7022255988440484 2214 8 -0.43735402910496646 2214 9 -4.988886810645809 2214 10 -1.472449764719702 2214 11 -0.8499183392793697 2214 12 -1.3770410993992854 2214 13 -1.454447303692966 2214 14 -2.884802903952675 2214 15 -2.3112958176051634 2214 16 -1.5758536594882386 2214 17 -1.5925248458179908 2214 18 -1.116569457681365 2214 19 -4.595710200207414 2214 20 -2.388374648537148 2214 21 -4.215248861035665 2214 22 -6.298792995843382 2214 23 -6.826341679695783 2214 24 -6.188371445749166 2214 25 -3.8853792537521663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27450.0 3 30.0 4 505.0 5 3284.0 6 3986.0 7 3176.0 8 2443.0 9 1989.0 10 1729.0 11 1625.0 12 1559.0 13 1454.0 14 1415.0 15 1505.0 16 1540.0 17 1662.0 18 1817.0 19 2072.0 20 2738.0 21 3726.0 22 4543.0 23 5574.0 24 6616.0 25 7883.0 26 8940.0 27 9870.0 28 11235.0 29 13705.0 30 16670.0 31 21057.0 32 26869.0 33 34199.0 34 44443.0 35 58375.0 36 78873.0 37 115588.0 38 185183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.1451520150441 20.23089440449756 9.29300306842461 30.330950512033738 2 12.479793488049491 21.636210069415313 41.860201701542124 24.023794740993072 3 18.12785190178061 26.001652931856416 29.482738608824306 26.387756557538673 4 11.67374976051731 18.525401946347486 38.5777595048407 31.223088788294508 5 12.234247839583858 39.82800570517661 35.59205190592052 12.345694549319013 6 30.64139917141029 39.06314909001089 18.08947197053903 12.205979768039791 7 27.102468497477133 32.001630999071004 22.46701220306779 18.42888830038407 8 23.489947932138033 40.67038072872588 19.970463118130713 15.869208221005374 9 26.05455093218617 15.775070238003488 20.109998668805922 38.06038016100442 10 15.093160407036734 30.38108660576216 34.03451814691465 20.491234840286456 11 33.34866483154691 22.52136507186465 25.98912628634299 18.14084381024545 12 24.253345841063286 25.028974221680407 32.19347090904262 18.524209028213694 13 29.66065916614476 23.226408025409324 25.552334034177328 21.560598774268588 14 19.862669686739707 24.482737876220842 27.463824179144986 28.190768257894465 15 21.645251619234816 34.40816320824485 22.764351530182143 21.182233642338186 16 20.216439443928735 28.831238186840842 30.543097759833582 20.409224609396844 17 20.05843742791436 30.848111618283365 28.714166882195457 20.379284071606822 18 20.057095084845756 28.268497884797668 32.24208651499095 19.43232051536562 19 23.533572856523747 27.046455382980806 27.3004711244076 22.11950063608785 20 23.421396977000374 28.756940988318448 28.455675533610318 19.365986501070857 21 21.614900059553264 27.225209205313384 27.142589532604717 24.01730120252864 22 22.720765858459334 30.221520756911513 26.904021651605976 20.153691733023173 23 20.882308535385167 30.02189205511318 28.294013375682205 20.80178603381945 24 23.444727920875128 27.581169398525144 29.047076503687137 19.927026176912594 25 21.763333412551184 28.968290026798933 28.73594873393567 20.532427826714216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 232.0 2 231.0 3 230.0 4 230.0 5 230.0 6 358.0 7 486.0 8 486.0 9 486.0 10 841.0 11 1196.0 12 1196.0 13 1196.0 14 2012.0 15 2828.0 16 2828.0 17 2828.0 18 5332.0 19 7836.0 20 7836.0 21 7836.0 22 13312.0 23 18788.0 24 18788.0 25 18788.0 26 28487.0 27 38186.0 28 38186.0 29 38186.0 30 52292.0 31 66398.0 32 66398.0 33 66398.0 34 73726.5 35 81055.0 36 81055.0 37 81055.0 38 84999.0 39 88943.0 40 88943.0 41 88943.0 42 91194.5 43 93446.0 44 93446.0 45 93446.0 46 94827.0 47 96208.0 48 96208.0 49 96208.0 50 89017.0 51 81826.0 52 81826.0 53 81826.0 54 69000.5 55 56175.0 56 56175.0 57 56175.0 58 47023.0 59 37871.0 60 37871.0 61 37871.0 62 30254.5 63 22638.0 64 22638.0 65 22638.0 66 17491.0 67 12344.0 68 12344.0 69 12344.0 70 8911.0 71 5478.0 72 5478.0 73 5478.0 74 3793.0 75 2108.0 76 2108.0 77 2108.0 78 1419.5 79 731.0 80 731.0 81 731.0 82 486.0 83 241.0 84 241.0 85 241.0 86 152.0 87 63.0 88 63.0 89 63.0 90 41.0 91 19.0 92 19.0 93 19.0 94 10.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08793169007783842 2 0.02963675404849244 3 0.03285206227073455 4 0.03425002236736155 5 0.026281649816587635 6 0.39031045897825895 7 0.23136239599176883 8 0.2298246398854791 9 0.23527668426232443 10 0.015377561062897019 11 0.0034949002415675047 12 0.005172452357519907 13 0.0 14 0.0775867853627986 15 0.002935716202916704 16 0.003355104231904804 17 0.004613268318869107 18 0.004193880289881006 19 0.0025163281739286033 20 0.001677552115952402 21 2.795920193254004E-4 22 0.0 23 0.0 24 4.193880289881005E-4 25 1.397960096627002E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 715328.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.25797074371337 #Duplication Level Percentage of deduplicated Percentage of total 1 92.01570754134877 72.00962548732959 2 5.7628530140826175 9.019783651527957 3 1.0353337839021854 2.430693629117859 4 0.38274281406174554 1.1981070378084244 5 0.1966313058384273 0.76939834898009 6 0.11606530714324316 0.5449821246465617 7 0.08196258802165983 0.4489958070834661 8 0.0582045340182443 0.36439749762809853 9 0.047467400577733 0.3343232201123117 >10 0.24754053537561121 3.6104267113789454 >50 0.02801521720624502 1.5613861944008842 >100 0.02208851560031549 3.426146184815995 >500 0.003950791403729306 2.082333483319732 >1k 0.0014366514195379293 2.1994006218501587 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2547 0.35606043661089737 No Hit GTCCTACAGTGGACATTTCTAAATT 2409 0.33676858727744474 No Hit CTTTAGGACGTGAAATATGGCGAGG 2376 0.33215531895857564 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2230 0.3117451015478214 No Hit GTCCTACAGTGTGCATTTCTCATTT 1682 0.2351368882526617 No Hit GTATCAACGCAGAGTACTTTTTTTT 1579 0.22073789925740359 No Hit CTGTAGGACCTGGAATATGGCGAGA 1563 0.21850116310280038 No Hit CTGAAGGACCTGGAATATGGCGAGA 1264 0.17670215621365304 No Hit GTACATGGGAAGCAGTGGTATCAAC 974 0.13616131341146998 No Hit GGTATCAACGCAGAGTACTTTTTTT 951 0.1329460051892279 No Hit CCCATGTACTCTGCGTTGATACCAC 924 0.12917151292833498 No Hit GATATACACTGTTCTACAAATCCCG 830 0.11603068802004116 No Hit GAATATGGCAAGAAAACTGAAAATC 785 0.10973986758521966 No Hit TATCAACGCAGAGTACTTTTTTTTT 762 0.10652455936297754 No Hit GTCCTTCAGTGTGCATTTCTCATTT 745 0.10414802719871165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCAT 40 0.005341483 14.223288 10 AAAGGCG 55 1.8921736E-4 13.873826 5 AAGGCGT 55 1.8921736E-4 13.873826 6 TAGGACC 565 0.0 13.843132 4 CCAACGA 90 5.585953E-7 12.629665 19 TGTAGGA 1480 0.0 12.505489 2 AGGACTA 70 1.04918145E-4 12.263471 5 GGACCTG 1010 0.0 12.182525 6 CTGTAGG 1410 0.0 12.105441 1 TCCAACG 110 3.9584847E-8 12.055591 18 AGGACCT 1055 0.0 12.024531 5 GACGTGG 690 0.0 11.982638 7 CTATAGG 145 9.094947E-11 11.83726 1 GGTCTAC 90 7.141136E-6 11.654587 1 CTCTAGG 140 6.366463E-10 11.578908 1 GGCGAGG 590 0.0 11.559355 19 GTAGGAC 1470 0.0 11.486456 3 AATCCCG 215 0.0 11.454813 19 GGACGTG 1375 0.0 11.445908 6 CTACTAT 75 2.0001437E-4 11.442685 1 >>END_MODULE