FastQCFastQC Report
Thu 26 May 2016
SRR1926651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687159
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10540.15338516995338777No Hit
GTCCTAAAGTGTGTATTTCTCATTT9040.13155616094673867No Hit
CTGTAGGACGTGGAATATGGCAAGA7620.1108913657537775No Hit
CTTTAGGACGTGAAATATGGCGAGG6920.10070449488400793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG400.002803832815.6693815
TGTAGAA2450.015.112042
GTCCTAG902.8217037E-614.8617371
TCTAGGA751.20511206E-414.6269632
CAATACA601.4548596E-414.3081644
TGTAGGA7000.013.9086742
GACTGGT757.061828E-613.6041397
TAGGACC2800.013.4139034
GATATAC2500.013.3755631
GTATAAA802.575016E-413.3755631
TCCTAGA1051.0744323E-513.0597882
CTGTAGG6700.012.7766581
GTACAGA957.708391E-512.6715861
GTCTTAA957.708391E-512.6715861
TGCAGAA1009.8648685E-512.34152
TAGAACA3000.012.161944
TCCAACG1057.1377144E-912.14496118
GTCCTAC7400.012.1103081
GTCCTAA3450.012.0186211
GTAGAAC2750.011.9637253
Produced by FastQC (version 0.11.5)