FastQCFastQC Report
Thu 26 May 2016
SRR1926651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687159
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA22900.33325620416817653No Hit
GTCCTACAGTGGACATTTCTAAATT21280.30968087444099546No Hit
CTTTAGGACGTGAAATATGGCGAGG20920.30444191227939965No Hit
GTCCTAAAGTGTGTATTTCTCATTT19000.2765007807508888No Hit
GTCCTACAGTGTGCATTTCTCATTT13590.19777082160024098No Hit
GTATCAACGCAGAGTACTTTTTTTT12860.18714737055033842No Hit
CTGAAGGACCTGGAATATGGCGAGA11920.17346785823950497No Hit
CTGTAGGACCTGGAATATGGCGAGA11630.16924758316488614No Hit
GGTATCAACGCAGAGTACTTTTTTT7660.11147347266062149No Hit
GATATACACTGTTCTACAAATCCCG7020.10215976215111787No Hit
GTCCTTCAGTGTGCATTTCTCATTT7000.10186870869769588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATAC400.00513052314.3098073
TAGGACC5050.013.7892984
GTCGAGG552.0088066E-413.776216519
TGTAGGA13800.013.2085752
TAGGACT1301.2732926E-1113.2080834
TGTCGAG655.5976016E-513.11485318
TTAGGGT905.127713E-712.7188964
GTAGGAC14050.012.6971953
CTGTAGG13750.012.6245441
GTCCTAA5050.012.4652091
GGACGTG12650.012.4423986
GACGTGG6050.012.4051967
AGGACGT13050.012.2803135
GGACTTG1251.3151293E-912.210146
GGACTGA550.002972375412.1407646
TGCCGGT802.918543E-511.851871510
GCCGGTT802.9250774E-511.84928311
TCCAACG1201.0419171E-811.83979818
TAGGACG14000.011.6514184
GACGTGA7100.011.6410687
Produced by FastQC (version 0.11.5)