FastQCFastQC Report
Thu 26 May 2016
SRR1926650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609574
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10020.16437708957403038No Hit
GTCCTAAAGTGTGTATTTCTCATTT8640.14173832873449327No Hit
CTGTAGGACGTGGAATATGGCAAGA7270.11926361688654699No Hit
CTTTAGGACGTGAAATATGGCGAGG6840.1122095102481405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT200.00900980426.7448521
GTCCTAT754.2134525E-717.82991
TCCTATA851.2036126E-616.1276192
GTCTAGA500.00239009216.0469111
GTATAAG500.00239009216.0469111
TAGGACC2400.015.6456444
TAGGACA2150.015.4689174
TGTAGGA5800.015.1265952
TAAGACT651.7289309E-514.8547654
CTGTAGG5450.014.7219371
GCCTTAT550.00450866714.58811
GATTAAA852.7159573E-514.15903951
TCCAACG553.188684E-513.92298518
AGGACCT4200.013.6782635
GTCTTAG700.001435450313.3724261
ATAGTGG550.001501752213.2939146
ATAGGAC500.00890178813.1652263
TTAGTAC500.00890178813.1652263
CCATTTC701.2090641E-512.91296113
TCTTACA853.490245E-412.9020962
Produced by FastQC (version 0.11.5)