FastQCFastQC Report
Thu 26 May 2016
SRR1926650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609574
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA20460.3356442367948764No Hit
CTTTAGGACGTGAAATATGGCGAGG19500.31989553360215495No Hit
GTCCTACAGTGGACATTTCTAAATT18730.3072637612496596No Hit
GTCCTAAAGTGTGTATTTCTCATTT18560.30447492839261514No Hit
GTATCAACGCAGAGTACTTTTTTTT15410.2527994960414978No Hit
GTCCTACAGTGTGCATTTCTCATTT11310.18553940948924985No Hit
CTGAAGGACCTGGAATATGGCGAGA10050.16486923654880295No Hit
CTGTAGGACCTGGAATATGGCGAGA9710.15929157083471407No Hit
GGTATCAACGCAGAGTACTTTTTTT9490.15568249301971543No Hit
GTACATGGGAAGCAGTGGTATCAAC9390.15404200310380692No Hit
CCCATGTACTCTGCGTTGATACCAC8780.14403501461676516No Hit
TATCAACGCAGAGTACTTTTTTTTT7770.12746606646608943No Hit
GAATATGGCAAGAAAACTGAAAATC6460.10597564856768826No Hit
GTCCTTCAGTGTGCATTTCTCATTT6260.10269466873587128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTAA350.00211570916.3455091
GTGTAGG1055.456968E-1215.4374241
ATAGGAC1058.185452E-1114.5365413
TAGGACC3900.014.4293274
CTTATAC400.005130588514.3094073
TAGTACC400.005136216514.3070444
TGGCGAA400.00536854614.21197718
ATTAAAC500.001451475113.3554483
CCTAATC500.001451475113.3554483
TGTAGGA11850.012.7226272
GTAGGAC12100.012.4566743
GGACCTA701.04972896E-412.2621696
CTGTAGG11850.012.1499311
ATGTCGA550.00312670512.05864817
TGTCGAG550.00312670512.05864818
TCGTCAT802.938927E-511.84331416
TAAGACT657.7469496E-411.7391134
GTATTAG657.768455E-411.7352371
GTCTTAT657.768455E-411.7352371
GACGTGG5950.011.6549587
Produced by FastQC (version 0.11.5)