FastQCFastQC Report
Thu 26 May 2016
SRR1926649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201976
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50980.12132387238765763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.010.6601321
GTATCAA36100.010.6244011
CCCTATA1951.5279511E-109.7786632
GCGCGCA1106.975568E-59.483249
GTACTAA2650.09.3608031
GTATTAA5100.09.1666681
GTACCGT2201.6189006E-109.0853846
AACCCGT1054.4111334E-49.0647836
CGCGCAC1054.5749903E-49.03176510
TATACGC850.00722591358.9729313
GTCCTAA3850.08.9212841
CCTATAC3550.08.86234953
GTGCTAG6150.08.6875741
CTAGCAC4950.08.6670363
CTAAGAC4100.08.6036193
GCGGTAT1000.002795878.5867371
GTCCTAC4800.08.5469831
GTCCTAG5400.08.4807271
GGATCGT1803.9239058E-78.4604646
TTATACC3400.08.4033754
Produced by FastQC (version 0.11.5)