##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926649_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4201976 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.632150921376038 31.0 28.0 31.0 16.0 34.0 2 27.600476299721844 31.0 28.0 31.0 16.0 34.0 3 27.245391453925485 31.0 27.0 31.0 10.0 34.0 4 31.051552650467304 35.0 32.0 37.0 16.0 37.0 5 31.287228913254147 35.0 32.0 37.0 16.0 37.0 6 30.73206724645738 35.0 31.0 37.0 10.0 37.0 7 30.489167239413074 35.0 30.0 37.0 10.0 37.0 8 30.36983766685007 35.0 30.0 37.0 10.0 37.0 9 32.4117884062165 37.0 32.0 39.0 11.0 39.0 10 32.64612886889406 37.0 32.0 39.0 11.0 39.0 11 32.80865883098809 37.0 33.0 39.0 12.0 39.0 12 32.687880892227845 37.0 33.0 39.0 10.0 39.0 13 32.75840271339008 37.0 33.0 39.0 10.0 39.0 14 33.73613057285429 39.0 33.0 40.0 10.0 41.0 15 33.29114469002203 38.0 33.0 40.0 10.0 41.0 16 33.362186742618235 38.0 33.0 40.0 10.0 41.0 17 33.567874495237476 38.0 33.0 40.0 8.0 41.0 18 33.59851246175609 39.0 33.0 40.0 7.0 41.0 19 32.938359476589106 38.0 32.0 40.0 2.0 41.0 20 33.248934072921884 39.0 33.0 40.0 2.0 41.0 21 33.23412175605001 39.0 33.0 40.0 2.0 41.0 22 33.246402406867624 39.0 33.0 40.0 2.0 41.0 23 33.223926076683924 39.0 33.0 40.0 2.0 41.0 24 31.87532913086605 38.0 31.0 40.0 2.0 41.0 25 32.438553432956304 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3949319848008379 1101 2 -0.57113009544641 1101 3 -1.5006858349059335 1101 4 -0.848382354047363 1101 5 -0.46439110198409495 1101 6 -2.544514888741226 1101 7 -0.9178562905503149 1101 8 -0.3447916328352285 1101 9 -0.5366963347938274 1101 10 -9.15356808633732 1101 11 -4.14284544011727 1101 12 -1.72547778634965 1101 13 -1.0200766404060104 1101 14 -0.7779684603381511 1101 15 -2.2063418192448836 1101 16 -0.5803487227983695 1101 17 -0.47879008810338064 1101 18 -0.38686722281237707 1101 19 -1.1323749299023511 1101 20 -0.3242042220110264 1101 21 -0.4919936517159442 1101 22 -0.2933360247495216 1101 23 -0.3563387917042675 1101 24 -0.3616807851367092 1101 25 -0.29770052895234755 1102 1 -0.24613834111423927 1102 2 -0.4110108603075453 1102 3 -0.6814072446013526 1102 4 -0.44905936554731696 1102 5 -0.1696482613429815 1102 6 -1.1646168859365211 1102 7 -0.3635217301581868 1102 8 0.19471946402634543 1102 9 0.097822156254594 1102 10 0.14281436407594583 1102 11 -0.048993219670038 1102 12 -0.04097194624434053 1102 13 -0.17275440269396114 1102 14 -0.4222305548051395 1102 15 0.2139168263931026 1102 16 0.0880368804283691 1102 17 -0.14286915112326426 1102 18 -0.3374515950940733 1102 19 0.17459002409120927 1102 20 0.0064849195108607205 1102 21 0.011392025928103067 1102 22 -0.12948150563419603 1102 23 -0.5518728640841957 1102 24 -4.069341520330141 1102 25 -2.131352616440651 1103 1 -0.3137068608285034 1103 2 -0.5547563677940524 1103 3 -0.281461966969097 1103 4 -0.3200930091432781 1103 5 -0.476563635587123 1103 6 0.24072016933002516 1103 7 -0.049156042605648764 1103 8 0.27162124397542087 1103 9 -0.028286606227993616 1103 10 -1.686344255347194E-4 1103 11 -0.22943032854217194 1103 12 -0.224385687954765 1103 13 -0.22802962879175936 1103 14 -0.3563091885124905 1103 15 -0.011940964488260875 1103 16 -0.0946977049202502 1103 17 -0.42093538041709166 1103 18 -0.4807913550060334 1103 19 -2.1233718469061387 1103 20 -0.8021265435561133 1103 21 -0.6109328726476946 1103 22 -0.4308327623196675 1103 23 -0.36644987744092106 1103 24 1.1115154989556828 1103 25 0.294835031533097 1104 1 -0.5114297524430533 1104 2 -0.48745490279003434 1104 3 -1.7030353083444894 1104 4 -0.8307415153842221 1104 5 -0.5192337812459122 1104 6 -1.791747799362053 1104 7 -0.4999445518122698 1104 8 0.13073854448860445 1104 9 -0.03284152434126497 1104 10 0.10589449540496076 1104 11 0.04962753821479993 1104 12 0.031191186182333297 1104 13 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1110 8 1.0168475170012954 1110 9 0.7613351835954845 1110 10 0.7301695745984915 1110 11 0.3514848478854802 1110 12 0.42885858318869907 1110 13 0.36841996879568484 1110 14 0.1989428235502757 1110 15 0.6805082777468101 1110 16 0.5368419792963408 1110 17 0.02209755210436981 1110 18 0.22537616962853235 1110 19 -2.1374020230456665 1110 20 -0.26118826922407834 1110 21 -0.10053449382285606 1110 22 0.12455346729559125 1110 23 0.16773910279380289 1110 24 -0.9562458771223774 1110 25 -0.5034030929746898 1111 1 0.47111263687917315 1111 2 0.1458263239776194 1111 3 0.7460187381239898 1111 4 0.6162346390406448 1111 5 0.6457363990139555 1111 6 0.7252831625155345 1111 7 0.7417433932045476 1111 8 1.0578699584492455 1111 9 0.4958822470346007 1111 10 0.4062495051898125 1111 11 0.26886052235601454 1111 12 0.40877223713389554 1111 13 0.24188221054389913 1111 14 0.3973871197446712 1111 15 -0.5629189255236042 1111 16 -0.22144517952209952 1111 17 0.011475858205400868 1111 18 0.23087413426282666 1111 19 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0.1361175991996788 2215 3 0.45514479692588594 2215 4 0.7625793695977485 2215 5 0.6644529549304394 2215 6 1.223810387291227 2215 7 0.8332017975418715 2215 8 -0.45394568341721353 2215 9 0.08933896845697831 2215 10 -0.202038433727175 2215 11 -0.18364501194380267 2215 12 -0.3916981061435223 2215 13 -0.03438143586167541 2215 14 0.07972922130878146 2215 15 -0.01901801596648056 2215 16 0.2162738738093566 2215 17 -0.22198590811075292 2215 18 -1.03513894266667 2215 19 0.18153459848229403 2215 20 -3.6700907320777496 2215 21 -2.166123851743251 2215 22 -1.1796340381292154 2215 23 -1.0012976773203661 2215 24 -0.9319917646343434 2215 25 -0.6323697257349501 2216 1 -0.1855238851038976 2216 2 -0.640078082180441 2216 3 0.2824110539037221 2216 4 -0.12022774639562428 2216 5 -0.3223850456635198 2216 6 -1.5276651591912973 2216 7 -9.352806961675206 2216 8 -10.738401810620637 2216 9 -5.6076801929394335 2216 10 -3.20287857272713 2216 11 -2.0352870056550323 2216 12 -1.7300765517146495 2216 13 -1.3903781411430103 2216 14 -1.5109468460729971 2216 15 -2.5984644808880795 2216 16 -1.6590570211659568 2216 17 -1.8632775129360866 2216 18 -1.5667999246245046 2216 19 -0.8457455271172662 2216 20 -1.829367693207221 2216 21 -4.324195716397863 2216 22 -2.945194970400074 2216 23 -2.8606497690638584 2216 24 -2.994871941049869 2216 25 -2.35741966525136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 293200.0 3 14.0 4 2096.0 5 12500.0 6 15766.0 7 12777.0 8 10455.0 9 9367.0 10 8769.0 11 8639.0 12 8684.0 13 8361.0 14 8725.0 15 8776.0 16 9607.0 17 10240.0 18 11933.0 19 14654.0 20 19193.0 21 24282.0 22 30125.0 23 36005.0 24 41516.0 25 47924.0 26 54477.0 27 59009.0 28 66290.0 29 77934.0 30 92386.0 31 112491.0 32 143850.0 33 186457.0 34 250002.0 35 344293.0 36 490228.0 37 754484.0 38 916467.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.001116040555196 17.072964251408642 11.820848782228056 26.105070925808104 2 16.48015622274894 19.199925472065843 38.452578027983236 25.867340277201983 3 19.047557779366215 23.293114129139862 28.387181319990468 29.272146771503454 4 12.924810907394454 16.16333657447859 35.63302234056411 35.278830177562845 5 14.260132233671216 36.478207342548814 34.94185260439776 14.319807819382211 6 34.560628562029635 34.74189143222899 17.13090035636066 13.566579649380719 7 30.204081730076123 30.506879422264106 21.035011490368046 18.25402735729173 8 26.94038229111176 34.74656650188931 19.685357345385185 18.627693861613746 9 27.069911630389093 15.105238528445799 18.583586950260614 39.241262890904494 10 15.712058438492956 27.851867828713544 32.93954866053457 23.49652507225893 11 35.70463402483543 22.151995900352674 22.584453472787352 19.558916602024546 12 23.84858222868904 23.992776482007734 29.82469377762999 22.333947511673237 13 29.056649580909017 20.99555976583745 24.88194879985148 25.06584185340206 14 22.816717627496093 19.969456825633035 26.012992274233525 31.20083327263734 15 24.676904240648206 27.90883770260576 22.592853882028454 24.821404174717582 16 24.59745890803216 26.86512808525973 24.527145429885753 24.010267576822354 17 23.659578033401147 26.908790824027136 25.63311770615912 23.7985134364126 18 23.784681685033213 26.738133507751844 25.98126476743763 23.49592003977732 19 24.96284840554616 26.103496974909255 25.67862508036718 23.255029539177407 20 24.743536920948273 25.863598463994485 25.897659455305007 23.49520515975224 21 24.807908262479597 25.579726461175355 25.647228123725117 23.965137152619924 22 24.830325600614287 25.361068359610233 25.711723231147065 24.096882808628415 23 24.531735717380023 25.591132184154276 25.579254794990074 24.297877303475623 24 24.607031260413315 25.93055759193707 25.331155304292523 24.131255843357092 25 24.50484592046069 25.482051207594473 25.68308091957645 24.330021952368384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 958.0 1 958.0 2 1297.5 3 1637.0 4 1637.0 5 1637.0 6 2372.0 7 3107.0 8 3107.0 9 3107.0 10 4505.0 11 5903.0 12 5903.0 13 5903.0 14 9315.5 15 12728.0 16 12728.0 17 12728.0 18 22355.0 19 31982.0 20 31982.0 21 31982.0 22 49163.0 23 66344.0 24 66344.0 25 66344.0 26 93412.0 27 120480.0 28 120480.0 29 120480.0 30 157406.5 31 194333.0 32 194333.0 33 194333.0 34 239216.0 35 284099.0 36 284099.0 37 284099.0 38 334857.0 39 385615.0 40 385615.0 41 385615.0 42 432353.0 43 479091.0 44 479091.0 45 479091.0 46 517766.0 47 556441.0 48 556441.0 49 556441.0 50 568739.5 51 581038.0 52 581038.0 53 581038.0 54 551342.5 55 521647.0 56 521647.0 57 521647.0 58 461861.0 59 402075.0 60 402075.0 61 402075.0 62 338039.0 63 274003.0 64 274003.0 65 274003.0 66 216680.5 67 159358.0 68 159358.0 69 159358.0 70 117501.0 71 75644.0 72 75644.0 73 75644.0 74 52863.0 75 30082.0 76 30082.0 77 30082.0 78 20441.5 79 10801.0 80 10801.0 81 10801.0 82 7062.0 83 3323.0 84 3323.0 85 3323.0 86 2166.0 87 1009.0 88 1009.0 89 1009.0 90 607.0 91 205.0 92 205.0 93 205.0 94 124.0 95 43.0 96 43.0 97 43.0 98 36.5 99 30.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20418964791802716 2 0.11642141697144391 3 0.11842047646155048 4 0.06765864440920177 5 0.022084847700224845 6 0.021299502900540127 7 0.29462329151808575 8 0.28748379333913376 9 0.01803913206548538 10 0.03467416282244354 11 0.015207131121167757 12 0.016635030756958154 13 0.014778761230430634 14 0.01689681235685306 15 0.014041013085272261 16 0.018800678537906925 17 0.01768215715653778 18 0.013969618103482743 19 0.022489419263698792 20 0.016397047484326424 21 0.014231399703377648 22 0.016516039120642288 23 0.017039602320432102 24 0.014850156212220156 25 0.014445584648746209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4201976.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.48345370214358 #Duplication Level Percentage of deduplicated Percentage of total 1 87.59683547320034 66.12111674895607 2 7.630171025257976 11.519033226489956 3 2.1639252848059014 4.900216621516323 4 0.8927515516547555 2.6955188166739434 5 0.48726880094292224 1.8390365988237043 6 0.29595861797684575 1.3403987182683381 7 0.19796424763682063 1.0460117584821544 8 0.12877280209841438 0.7776172676232768 9 0.10168457449684888 0.6907952584129555 >10 0.4742656450706862 6.232254524919711 >50 0.021595135240822926 1.0997165879395607 >100 0.008052953644355616 0.9804374355597942 >500 4.0835608025673845E-4 0.22134091703371753 >1k 3.1411990282892335E-4 0.415627579786025 >5k 3.141199028289198E-5 0.12087793951446968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5098 0.12132387238765763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.3798327263173326E-5 0.0 3 0.0 0.0 0.0 2.3798327263173326E-5 0.0 4 0.0 0.0 0.0 2.3798327263173326E-5 2.3798327263173326E-5 5 0.0 1.1899163631586663E-4 0.0 2.3798327263173326E-5 2.3798327263173326E-5 6 0.0 1.1899163631586663E-4 0.0 2.3798327263173326E-5 4.759665452634665E-5 7 0.0 1.1899163631586663E-4 0.0 2.3798327263173326E-5 4.759665452634665E-5 8 0.0 1.1899163631586663E-4 0.0 2.3798327263173326E-5 4.759665452634665E-5 9 0.0 1.1899163631586663E-4 0.0 2.3798327263173326E-5 4.759665452634665E-5 10 0.0 1.1899163631586663E-4 0.0 2.3798327263173326E-5 7.139498178951998E-5 11 0.0 1.4278996357903995E-4 0.0 7.139498178951998E-5 7.139498178951998E-5 12 0.0 1.6658829084221328E-4 0.0 7.139498178951998E-5 9.51933090526933E-5 13 0.0 1.903866181053866E-4 0.0 7.139498178951998E-5 9.51933090526933E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1790 0.0 10.660132 1 GTATCAA 3610 0.0 10.624401 1 CCCTATA 195 1.5279511E-10 9.778663 2 GCGCGCA 110 6.975568E-5 9.48324 9 GTACTAA 265 0.0 9.360803 1 GTATTAA 510 0.0 9.166668 1 GTACCGT 220 1.6189006E-10 9.085384 6 AACCCGT 105 4.4111334E-4 9.064783 6 CGCGCAC 105 4.5749903E-4 9.031765 10 TATACGC 85 0.0072259135 8.972931 3 GTCCTAA 385 0.0 8.921284 1 CCTATAC 355 0.0 8.8623495 3 GTGCTAG 615 0.0 8.687574 1 CTAGCAC 495 0.0 8.667036 3 CTAAGAC 410 0.0 8.603619 3 GCGGTAT 100 0.00279587 8.586737 1 GTCCTAC 480 0.0 8.546983 1 GTCCTAG 540 0.0 8.480727 1 GGATCGT 180 3.9239058E-7 8.460464 6 TTATACC 340 0.0 8.403375 4 >>END_MODULE