FastQCFastQC Report
Thu 26 May 2016
SRR1926649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201976
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93650.2228713348196182No Hit
GGTATCAACGCAGAGTACTTTTTTT58320.13879184459882685No Hit
TATCAACGCAGAGTACTTTTTTTTT52860.1257979579131342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.012.7683661
TTAGACA3700.010.5528244
GTATCAA45200.010.1233761
TTATACG1053.9997E-59.976774
GTCTAGG4150.09.4179921
TAGGACG1752.5695044E-89.2511874
CAGCGTA1252.7977563E-59.1085139
TAGACAG6200.09.0624765
GCACCGT3050.09.0547066
CTAATAG2451.6370905E-118.9410773
ACCCGTG1606.867904E-78.8954557
ATTGCAC2900.08.867353
GTATTAG2502.3646862E-118.7702181
CTTAGAC3600.08.7305093
TCTTATA4050.08.7084992
GTCCTAA3950.08.6881641
AAGGCGT3100.08.6014676
GTCCTAC3900.08.5551181
GGCTTAA2803.6379788E-128.5114691
ACTAGAC3050.08.4312513
Produced by FastQC (version 0.11.5)