FastQCFastQC Report
Thu 26 May 2016
SRR1926648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4123389
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46990.11395965794156215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.012.0745491
GTATCAA38900.010.1519321
CGCGCAC1054.1824467E-59.93627410
TAGGCCG1106.709024E-59.5170645
TAGGACT4850.09.4229654
GCTTAGG3100.09.2311861
ACCGACC1252.7248172E-59.1289388
GGACCGT2106.4574124E-109.0638726
GCGATAC1054.575468E-49.03166114
TACGGTT950.00179522969.0161675
GTGTAGA6350.09.0131271
TGGACCG2750.08.9979525
GTACTAA2356.002665E-118.9300271
GCACCGT2356.366463E-118.9095936
CCGATCA1304.4574885E-58.7548269
GTATAAT4250.08.7533381
GTAGGAC4700.08.7201583
GTCCTAA3950.08.6936751
CCTACAC5700.08.5280363
GGTGCGA1356.9480324E-58.42924411
Produced by FastQC (version 0.11.5)