FastQCFastQC Report
Thu 26 May 2016
SRR1926648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4123389
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85220.20667465524111356No Hit
GGTATCAACGCAGAGTACTTTTTTT52650.12768623091345493No Hit
TATCAACGCAGAGTACTTTTTTTTT47440.11505099324851475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGG802.9041601E-511.86089410
GGTATCA23200.011.6263441
GTATCAA47850.09.9394721
TAGGACG2700.09.8735584
GTCTAGG4250.09.867531
ATTAGAC2251.8189894E-129.7332173
CTAGGAC4850.09.6197883
GTCTTAC2500.09.5311371
GTAGGAC4300.09.52162553
GCGAATC1106.9279144E-59.48917615
GTCTTAA4050.09.4134681
CGTTGAT4450.09.38164514
GTATTAG3050.09.3748881
TAAGACT4450.09.24
ATAAGAC2800.09.1815683
GCCGTAC1356.575352E-69.1781331
TAGGACA5950.09.1208934
CGTGCCG1151.1259534E-49.07616210
TAGGACC3700.09.0062864
GTCTTAG4250.08.9704821
Produced by FastQC (version 0.11.5)