FastQCFastQC Report
Thu 26 May 2016
SRR1926647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618324
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60020.12996056578100626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.013.28731
GTATCAA45350.010.6478831
GTCCTAG6250.09.7721461
TTAGGAC3700.09.5333443
TAGACTG5250.09.42684655
GTCCTAC3900.09.2984171
CCGCGCG1151.13349786E-49.0706559
GTATAAT5800.09.0495021
TAAACGG850.0073279868.9576385
GTCTTAG6400.08.9466671
GTATAAA6450.08.8773141
CTTACAC6000.08.7388993
TAGACAG6600.08.6522645
GTGCTAG6350.08.5662581
GCGATAC1901.04313585E-78.48438514
TTACACT5950.08.4810834
CTAGCAC5250.08.3530253
GTTTACG1050.00433488848.1798111
GTATTAT4600.08.09089851
ACCGACC1304.1451372E-48.0462648
Produced by FastQC (version 0.11.5)