##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926647_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4618324 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.07867291251112 31.0 28.0 31.0 16.0 34.0 2 28.126456913806827 31.0 28.0 31.0 16.0 34.0 3 27.73792072622016 31.0 28.0 31.0 16.0 34.0 4 31.441784075781605 35.0 32.0 37.0 19.0 37.0 5 31.580168260174037 35.0 32.0 37.0 19.0 37.0 6 30.734383295758374 35.0 30.0 37.0 11.0 37.0 7 30.79049131243282 35.0 31.0 37.0 12.0 37.0 8 30.665389219119316 35.0 31.0 37.0 11.0 37.0 9 32.91693783285885 37.0 32.0 39.0 17.0 39.0 10 33.187950433966954 37.0 33.0 39.0 17.0 39.0 11 33.36073324435444 37.0 33.0 39.0 18.0 39.0 12 33.25052508225928 38.0 33.0 39.0 17.0 39.0 13 33.356485166480304 38.0 34.0 39.0 18.0 39.0 14 34.396810834406594 39.0 34.0 40.0 18.0 41.0 15 33.9206147078464 39.0 33.0 40.0 11.0 41.0 16 34.027691430917365 39.0 33.0 40.0 17.0 41.0 17 34.26140132221126 39.0 34.0 40.0 17.0 41.0 18 34.308427256294706 39.0 34.0 41.0 17.0 41.0 19 33.635391107250165 39.0 33.0 40.0 10.0 41.0 20 33.91128340064491 39.0 34.0 40.0 10.0 41.0 21 33.910707434125456 39.0 34.0 40.0 10.0 41.0 22 33.93525530040768 39.0 34.0 40.0 10.0 41.0 23 33.933088713567955 39.0 34.0 41.0 2.0 41.0 24 32.575685898174314 38.0 32.0 40.0 2.0 41.0 25 33.15973110591634 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.31157686356433345 1101 2 -0.5266101338190197 1101 3 -1.4401439873395212 1101 4 -0.8272406232385663 1101 5 -0.6206615884970574 1101 6 -2.7452957568532597 1101 7 -0.9542783224291895 1101 8 -0.3540135720541606 1101 9 -0.35479379776341347 1101 10 -9.29331570640544 1101 11 -4.095440323124645 1101 12 -1.7316840984553963 1101 13 -0.9555377995006893 1101 14 -0.7749413358862753 1101 15 -2.3005416008414876 1101 16 -0.5335519860440598 1101 17 -0.4396500576056752 1101 18 -0.33367617745179246 1101 19 -1.1171954762352243 1101 20 -0.2835171683056501 1101 21 -0.4135428768866518 1101 22 -0.09252962117528796 1101 23 -0.20965896672450413 1101 24 -0.45536607755717284 1101 25 -0.27075636269781 1102 1 0.02635102029066516 1102 2 -0.2587641817582771 1102 3 -0.4229736931078065 1102 4 -0.3274597241759736 1102 5 -0.011556618213091951 1102 6 -1.1049597084322045 1102 7 -0.0947514595268224 1102 8 0.38900148596974304 1102 9 0.4536718178461143 1102 10 0.5361870315266088 1102 11 0.3782868854566743 1102 12 0.3901918038101755 1102 13 0.20113232722870578 1102 14 0.07579346260246211 1102 15 0.6021721503188573 1102 16 0.5699320757322681 1102 17 0.315644449832277 1102 18 0.13753315470700045 1102 19 0.5330971743342161 1102 20 0.4194705647240795 1102 21 0.3158192991222961 1102 22 0.25185247758484053 1102 23 -0.05054865700593325 1102 24 -3.5619658324023398 1102 25 -1.692022251039031 1103 1 -0.08576957578634747 1103 2 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-0.3017778744241255 2215 3 0.15798329744818318 2215 4 0.46630837749488663 2215 5 0.45777088761875007 2215 6 1.1696458085714987 2215 7 0.551950698079736 2215 8 -0.7229041049868918 2215 9 -0.2918357079709537 2215 10 -0.5957892660871451 2215 11 -0.6891734414033266 2215 12 -0.9440354391953107 2215 13 -0.5975027601189566 2215 14 -0.6379600605390081 2215 15 -0.5443012190498422 2215 16 -0.4065597730334787 2215 17 -0.8551372656223037 2215 18 -1.5151236068765144 2215 19 -0.32712063808401837 2215 20 -4.413258839098894 2215 21 -2.767901366869925 2215 22 -1.7187155981579423 2215 23 -1.543879808861277 2215 24 -1.4492563619190868 2215 25 -1.301574838626479 2216 1 -0.9501456112469668 2216 2 -1.4067096033976192 2216 3 -0.5541049728691156 2216 4 -0.8019986738154756 2216 5 -1.0264790699925008 2216 6 -1.9204159605454194 2216 7 -9.906568623036168 2216 8 -11.23217528249264 2216 9 -6.439695983857053 2216 10 -4.163484700422185 2216 11 -3.067352781211415 2216 12 -2.7618345596892695 2216 13 -2.4895013713714818 2216 14 -2.6557736323898915 2216 15 -3.701925543881277 2216 16 -2.779583464401256 2216 17 -3.107277277982675 2216 18 -2.831982113079416 2216 19 -2.1374394774139667 2216 20 -2.941240125057078 2216 21 -5.626378880237279 2216 22 -4.311118363990921 2216 23 -4.142235059485426 2216 24 -4.341030936604664 2216 25 -3.787192825998943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 267704.0 3 50.0 4 2037.0 5 11799.0 6 16306.0 7 13346.0 8 10290.0 9 9269.0 10 8522.0 11 8229.0 12 8169.0 13 8315.0 14 8372.0 15 8601.0 16 9224.0 17 10142.0 18 11783.0 19 14484.0 20 19085.0 21 24961.0 22 31483.0 23 37609.0 24 43870.0 25 50478.0 26 57939.0 27 63638.0 28 71792.0 29 84761.0 30 101137.0 31 123996.0 32 159705.0 33 208535.0 34 281916.0 35 390549.0 36 557355.0 37 860273.0 38 1022600.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60661677579752 17.31868841586797 12.327503665425038 25.747191142909475 2 15.915044370583809 19.30709040411596 39.30398999195627 25.473875233343957 3 18.661660499659813 23.533046643039363 29.55707693848763 28.248215918813198 4 12.901194616048153 16.538974150851452 35.934643562045906 34.62518767105448 5 14.262803749085784 36.41051696659049 35.10760201925828 14.219077265065442 6 33.284272470575395 34.85247306164473 17.68426086229592 14.178993605483958 7 29.061228266498002 31.58890448901292 21.284605052355023 18.065262192134064 8 26.629777544563193 34.8866525000391 19.873820871508226 18.609749083889486 9 26.878679877535756 15.515718316642884 18.973750143746273 38.63185166207509 10 15.818341396806032 27.993867486414548 32.84483908866586 23.342952028113565 11 35.137269730060304 22.462569494614545 22.85725299418056 19.54290778114459 12 23.792101726320748 24.187873004955424 29.845853854606517 22.174171414117307 13 28.763678494472295 21.251740603986878 25.135794181022813 24.848786720518014 14 22.684200222453992 20.335222921770345 26.266268247637726 30.714308608137937 15 24.574600739508433 28.09237661829731 22.825079011846256 24.507943630348 16 24.308734303099186 27.098637522869655 24.740160849919608 23.852467324111547 17 23.369584402359795 27.12201075945762 25.85083028353538 23.657574554647205 18 23.481355973312958 26.977208428836004 26.171694308300808 23.369741289550234 19 24.729495358322275 26.353086308523434 25.91451793430614 23.00290039884816 20 24.669112105572275 26.04944672269606 26.056939831634608 23.224501340097056 21 24.72974418656021 25.72366708520459 25.84210364465827 23.704485083576927 22 24.711385617574575 25.55853932146276 25.853780551717563 23.876294509245103 23 24.4124529376541 25.740106485156073 25.759619018331172 24.087821558858657 24 24.50570390375836 26.047534805214884 25.494025394428782 23.95273589659797 25 24.25227052918305 25.70041098862927 25.897849250462247 24.14946923172543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1504.0 1 1504.0 2 1760.0 3 2016.0 4 2016.0 5 2016.0 6 2776.0 7 3536.0 8 3536.0 9 3536.0 10 5038.0 11 6540.0 12 6540.0 13 6540.0 14 10769.5 15 14999.0 16 14999.0 17 14999.0 18 26406.0 19 37813.0 20 37813.0 21 37813.0 22 58059.0 23 78305.0 24 78305.0 25 78305.0 26 110374.5 27 142444.0 28 142444.0 29 142444.0 30 185434.5 31 228425.0 32 228425.0 33 228425.0 34 278802.0 35 329179.0 36 329179.0 37 329179.0 38 384111.5 39 439044.0 40 439044.0 41 439044.0 42 488991.5 43 538939.0 44 538939.0 45 538939.0 46 576983.5 47 615028.0 48 615028.0 49 615028.0 50 625623.5 51 636219.0 52 636219.0 53 636219.0 54 598513.0 55 560807.0 56 560807.0 57 560807.0 58 492181.5 59 423556.0 60 423556.0 61 423556.0 62 353284.5 63 283013.0 64 283013.0 65 283013.0 66 222065.5 67 161118.0 68 161118.0 69 161118.0 70 117508.0 71 73898.0 72 73898.0 73 73898.0 74 51191.0 75 28484.0 76 28484.0 77 28484.0 78 19142.5 79 9801.0 80 9801.0 81 9801.0 82 6296.5 83 2792.0 84 2792.0 85 2792.0 86 1739.5 87 687.0 88 687.0 89 687.0 90 414.5 91 142.0 92 142.0 93 142.0 94 80.5 95 19.0 96 19.0 97 19.0 98 17.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22518991738128377 2 0.13065345783448715 3 0.1330785800216702 4 0.06931085822475859 5 0.021328083521208126 6 0.020137175304287876 7 0.31119514351959715 8 0.3047858920248991 9 0.017603788733748436 10 0.035077660207469205 11 0.0151570136698941 12 0.016412880516828182 13 0.01459403887644089 14 0.016023128736745194 15 0.014334204356385565 16 0.018448250923928246 17 0.017430565720378214 18 0.014117675589672791 19 0.022259157218073047 20 0.01619635175011541 21 0.014334204356385565 22 0.0161097402434303 23 0.016867590926925007 24 0.015092055039880268 25 0.014680650383126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4618324.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.5265077711276 #Duplication Level Percentage of deduplicated Percentage of total 1 87.33539947692717 65.08802327811746 2 7.706375661462246 11.486585312423893 3 2.212218331176943 4.9460671994966825 4 0.9263409627889534 2.7614782784801903 5 0.5169027365885219 1.9261477907640803 6 0.3150780356532791 1.4088999403575433 7 0.20240760017336917 1.055931211107912 8 0.14433857628143956 0.8605640021529758 9 0.09959464685771087 0.6680197100703508 >10 0.5061267529413706 6.5264814161680285 >50 0.025152481358749625 1.2825672710288114 >100 0.009312463544043028 1.1659943080656952 >500 4.0507097353059945E-4 0.21289373140297888 >1k 3.1826966653289386E-4 0.48092418699002887 >5k 2.893360604843656E-5 0.1294223633732534 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6002 0.12996056578100626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.1652876671277285E-5 0.0 0.0 0.0 6 2.1652876671277285E-5 2.1652876671277285E-5 0.0 0.0 2.1652876671277285E-5 7 2.1652876671277285E-5 2.1652876671277285E-5 0.0 0.0 2.1652876671277285E-5 8 2.1652876671277285E-5 2.1652876671277285E-5 0.0 0.0 2.1652876671277285E-5 9 2.1652876671277285E-5 2.1652876671277285E-5 0.0 0.0 2.1652876671277285E-5 10 2.1652876671277285E-5 2.1652876671277285E-5 0.0 0.0 2.1652876671277285E-5 11 2.1652876671277285E-5 2.1652876671277285E-5 0.0 2.1652876671277285E-5 2.1652876671277285E-5 12 2.1652876671277285E-5 2.1652876671277285E-5 0.0 2.1652876671277285E-5 6.495863001383186E-5 13 2.1652876671277285E-5 2.1652876671277285E-5 0.0 2.1652876671277285E-5 6.495863001383186E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2090 0.0 13.2873 1 GTATCAA 4535 0.0 10.647883 1 GTCCTAG 625 0.0 9.772146 1 TTAGGAC 370 0.0 9.533344 3 TAGACTG 525 0.0 9.4268465 5 GTCCTAC 390 0.0 9.298417 1 CCGCGCG 115 1.13349786E-4 9.070655 9 GTATAAT 580 0.0 9.049502 1 TAAACGG 85 0.007327986 8.957638 5 GTCTTAG 640 0.0 8.946667 1 GTATAAA 645 0.0 8.877314 1 CTTACAC 600 0.0 8.738899 3 TAGACAG 660 0.0 8.652264 5 GTGCTAG 635 0.0 8.566258 1 GCGATAC 190 1.04313585E-7 8.484385 14 TTACACT 595 0.0 8.481083 4 CTAGCAC 525 0.0 8.353025 3 GTTTACG 105 0.0043348884 8.179811 1 GTATTAT 460 0.0 8.0908985 1 ACCGACC 130 4.1451372E-4 8.046264 8 >>END_MODULE