FastQCFastQC Report
Thu 26 May 2016
SRR1926647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618324
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113830.24647469514914935No Hit
GGTATCAACGCAGAGTACTTTTTTT70700.15308583806593043No Hit
TATCAACGCAGAGTACTTTTTTTTT67920.14706633835131533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.013.3422141
CGACCGG658.1108586E-411.67812110
GTACTAA2550.010.8412911
GTATCAA53250.010.3653061
TTAGACG856.467204E-410.0807854
GTCTAGG4050.09.6505491
CGTAGTA1151.0724213E-49.11689952
GTCCTAA3350.08.8214531
CTAGCAC4800.08.72823
TACGCAG1651.0188196E-68.6552195
CTTACAC5200.08.6061273
ACCGTGC4300.08.6054538
TAACGCA1000.00284982038.5686674
ACGGCGT1000.00293991328.5392578
GTAGGAC3950.08.4369253
GCCTACG2605.4569682E-118.4329151
TTAGGAC3300.08.3675313
AACCGTC1252.7783078E-48.350767
GTATTAG2303.4688128E-98.2894441
GTATTAA5300.08.2738031
Produced by FastQC (version 0.11.5)