FastQCFastQC Report
Thu 26 May 2016
SRR1926646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826683
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA1403.6379788E-1115.2890871
GTGCTAG902.819408E-614.864391
GTATAGA1005.1960706E-714.7157461
CGTACAC450.004355793414.6663733
GTTCAGG1059.016694E-714.0149961
GTATAAA1059.016694E-714.0149961
TAGCACT550.00119065113.7106294
GTCTTAA904.6392677E-513.3779521
GTATTGA802.5728584E-413.3779511
CCTAAGA750.001981516112.8132932
GGTATCA3500.012.6134961
GTTTAGA854.2940426E-412.5910121
GTTTAAC750.002406665112.4860881
GTCCTAC1307.316212E-712.3488781
TCCTATA905.588466E-412.2031372
GTGCAGG1001.2436774E-412.0401551
TAGGGCT902.636052E-511.9695974
GTATAGG906.9466204E-411.8915121
GTTCTGA800.00390234411.7057071
GTATCAA5700.011.5003441
Produced by FastQC (version 0.11.5)