FastQCFastQC Report
Thu 26 May 2016
SRR1926646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826683
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24710.29890538453070886No Hit
GGTATCAACGCAGAGTACTTTTTTT15040.18193188924896242No Hit
TATCAACGCAGAGTACTTTTTTTTT11680.14128753101249208No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8910.10778012853778292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAA551.8975469E-413.869681
GTCTATA853.7765385E-612.3399371
TAGGACA1501.2732926E-1112.083334
TGCGTTG1157.270137E-811.54016312
TAAGGCT951.2969209E-511.0457034
GTGTAGC1501.9972504E-910.8067931
ATTAAAC1158.3119085E-710.7863773
CTCTGCG1502.1227606E-910.7668059
TAGGACC1252.1097185E-710.6842084
TGGACAG1553.4051482E-910.46264655
GTATTAA1201.4490925E-610.3300221
CCTAAAC750.00255068110.1779173
TCTGCGT1409.958967E-810.15713610
CTAAGCT951.5744014E-410.0415494
ATTGGAG1053.9048005E-59.9961683
TAGAACA2500.09.921054
GGTATCA6350.09.7606511
ATAGGAC1106.504077E-59.5417963
CCTACAC1106.504077E-59.5417963
CTAGTAC800.0043511379.5417963
Produced by FastQC (version 0.11.5)