FastQCFastQC Report
Thu 26 May 2016
SRR1926645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765919
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC2800.017.1623611
TCCTACA3250.015.9854212
CTAGGGC505.134877E-415.3206343
GTCTAGG1109.8301825E-814.5620031
GCCTAGG658.318727E-414.375311
CTATAGG852.7654844E-514.1337081
GTAAGAC702.8881714E-514.0699693
GTGTAGG955.0513518E-614.0510541
GTCATAC802.6206876E-413.3485031
TAGGACT1055.5621058E-813.2802084
CTAGATA652.8799096E-413.2016274
TGTAGGA2500.013.1248712
GACAGTC705.654445E-513.0999247
TATAGGA1051.1088838E-513.0207062
GTAGGAC1950.012.9074953
CTAAAGT1253.0031515E-912.8715864
GTGTAGC957.860137E-512.6459491
GGACGTG1850.012.3921916
TAGGACG2350.012.3238594
GACGTGG1008.2631595E-712.2265977
Produced by FastQC (version 0.11.5)