##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926645_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 765919 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.163606073227065 27.0 7.0 31.0 2.0 31.0 2 16.35979522638817 16.0 2.0 30.0 2.0 31.0 3 19.83806773301093 23.0 15.0 31.0 2.0 31.0 4 21.976869616761043 28.0 2.0 35.0 2.0 37.0 5 22.6865543223239 30.0 2.0 35.0 2.0 37.0 6 23.41994127316335 31.0 2.0 35.0 2.0 37.0 7 23.1636700486605 30.0 2.0 35.0 2.0 37.0 8 23.153883112966252 30.0 7.0 35.0 2.0 37.0 9 25.933201813768818 31.0 17.0 37.0 2.0 39.0 10 26.697637739760992 32.0 23.0 37.0 2.0 39.0 11 23.854833213433796 30.0 7.0 37.0 2.0 39.0 12 24.989351354386038 31.0 10.0 37.0 2.0 39.0 13 21.697461480913777 27.0 2.0 35.0 2.0 39.0 14 25.001070609294196 31.0 15.0 38.0 2.0 40.0 15 26.05049750691653 31.0 17.0 38.0 2.0 40.0 16 26.417703438614264 31.0 16.0 39.0 2.0 40.0 17 25.74967979642756 31.0 10.0 38.0 2.0 40.0 18 25.818910354750308 31.0 11.0 38.0 2.0 40.0 19 26.221787160261073 31.0 12.0 39.0 2.0 40.0 20 26.457960959318154 31.0 11.0 39.0 2.0 40.0 21 24.34282345783301 30.0 2.0 38.0 2.0 40.0 22 25.254404186343464 31.0 2.0 38.0 2.0 40.0 23 25.257609486120597 31.0 2.0 38.0 2.0 40.0 24 24.310660787890104 31.0 2.0 39.0 2.0 40.0 25 24.121951537956363 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.230572924837144 1101 2 10.808296958026661 1101 3 7.246161938568886 1101 4 8.988900439119753 1101 5 8.947639381352442 1101 6 8.433357338436831 1101 7 8.279746456925167 1101 8 6.48787611860271 1101 9 6.477347276764032 1101 10 6.07486554828537 1101 11 8.903568984726672 1101 12 6.560651165832208 1101 13 11.001685141259628 1101 14 8.41834007574958 1101 15 7.6524186022457386 1101 16 7.0833823945042305 1101 17 3.074538514013298 1101 18 5.672904602143596 1101 19 6.433562936724872 1101 20 4.752767166915309 1101 21 1.89479162996723 1101 22 5.618443784132513 1101 23 6.696835004689412 1101 24 8.016290429640492 1101 25 8.46758157509204 1102 1 5.623338981476447 1102 2 10.525699192152848 1102 3 6.746372187759885 1102 4 8.146623897444705 1102 5 8.060945412761843 1102 6 7.100735119667981 1102 7 7.227293854405833 1102 8 7.175734913417781 1102 9 6.405139248901442 1102 10 5.841542651234345 1102 11 8.363075447603979 1102 12 5.659272890972527 1102 13 10.011028661013913 1102 14 7.752211220419301 1102 15 5.6977912917899545 1102 16 6.337951886432489 1102 17 6.996027581055365 1102 18 7.132093405917111 1102 19 5.873981286908613 1102 20 6.096285778539347 1102 21 4.977699513397763 1102 22 6.089026037272539 1102 23 6.444438849507225 1102 24 7.780411405196741 1102 25 7.296755250065072 1103 1 5.169294559513787 1103 2 9.558702962961272 1103 3 6.436678450980708 1103 4 8.217781811543851 1103 5 7.606894092978891 1103 6 7.372577067612429 1103 7 7.147628628617181 1103 8 6.768348497525203 1103 9 5.820182245314577 1103 10 5.0773710794510265 1103 11 5.93671427773366 1103 12 4.821738401871084 1103 13 8.938687770735243 1103 14 7.155664729110558 1103 15 6.284782002725297 1103 16 6.021508632500733 1103 17 3.618515624833531 1103 18 2.4745517890680375 1103 19 4.752417551049145 1103 20 5.003336500511203 1103 21 7.257630305267533 1103 22 3.1819960087615478 1103 23 5.158778268250902 1103 24 6.366365143815337 1103 25 2.784899046625359 1104 1 5.829299185930626 1104 2 10.58310152877205 1104 3 7.281658788709137 1104 4 7.727757522855441 1104 5 7.798468231308757 1104 6 7.6149145647209195 1104 7 7.586768115084912 1104 8 7.417342251862468 1104 9 6.368424206915318 1104 10 5.210039365363588 1104 11 8.347239376045017 1104 12 4.9977677796180195 1104 13 9.511443958567767 1104 14 7.386731118192216 1104 15 6.7157905615964495 1104 16 6.154604861971006 1104 17 6.5967714656847924 1104 18 4.119086834175601 1104 19 4.83248347748912 1104 20 4.1470387905875405 1104 21 3.2451100646938578 1104 22 5.430706395067354 1104 23 5.724837717707299 1104 24 6.6612529532069615 1104 25 5.509118338783583 1105 1 4.091906729116378 1105 2 9.884280291941176 1105 3 6.711147523349833 1105 4 6.195732116830776 1105 5 2.674443139770691 1105 6 5.545040696311098 1105 7 6.0975100788290995 1105 8 3.6008317053296857 1105 9 4.606653422720516 1105 10 4.4107857094568566 1105 11 7.425804867879599 1105 12 4.753316783369396 1105 13 6.1582199093281496 1105 14 4.7728847224332895 1105 15 5.126540387505656 1105 16 5.111708328062612 1105 17 5.953623904079116 1105 18 3.4515469056089856 1105 19 3.272268840384193 1105 20 2.4390513925148944 1105 21 3.7224529898685113 1105 22 5.007912293937448 1105 23 2.310575188785762 1105 24 5.4176493860303765 1105 25 6.003787790902997 1106 1 6.683678140309581 1106 2 11.255504126174648 1106 3 7.626463983514331 1106 4 9.234575704673624 1106 5 8.689918447758973 1106 6 7.781273206079561 1106 7 7.632355860672657 1106 8 7.275084509604728 1106 9 6.467242055733166 1106 10 5.813760644084869 1106 11 8.123829452635093 1106 12 5.570070476920716 1106 13 4.83041665254046 1106 14 6.10075043221153 1106 15 6.110878806684699 1106 16 6.09826953063568 1106 17 6.5343672032805316 1106 18 6.458305948394717 1106 19 6.632841452847092 1106 20 6.1874234059721545 1106 21 6.233896278480071 1106 22 6.531913521274479 1106 23 6.8438270616966435 1106 24 7.819394811348822 1106 25 7.101992131657376 1107 1 3.779234734640511 1107 2 -13.820686350015828 1107 3 -5.44559973743187 1107 4 1.6814394393527863 1107 5 4.376800035289442 1107 6 4.667759706841476 1107 7 4.6296140218817925 1107 8 5.201926377181994 1107 9 3.4799740965834474 1107 10 3.030392318106589 1107 11 5.4563805352943255 1107 12 3.133679710753455 1107 13 4.514242127714169 1107 14 3.9262911015878146 1107 15 3.507471975592214 1107 16 3.2798288028943006 1107 17 4.126080416767355 1107 18 -0.7765266547147434 1107 19 1.896678601038964 1107 20 2.0816278316834307 1107 21 -1.3485382060452586 1107 22 1.8339732015605499 1107 23 -1.2554046846872318 1107 24 2.3941515134306854 1107 25 3.20448440026356 1108 1 5.59373158943588 1108 2 10.734869205539729 1108 3 0.451124973738235 1108 4 6.1621695236581715 1108 5 7.525953347370031 1108 6 7.618006744799853 1108 7 7.492039370687294 1108 8 7.591521126703597 1108 9 6.178996820468065 1108 10 5.4260822444859365 1108 11 7.499390021573145 1108 12 2.828403608101528 1108 13 3.944712070147137 1108 14 5.220887249037087 1108 15 5.505873510288673 1108 16 5.281528067536598 1108 17 0.6783822757724245 1108 18 4.346334476158344 1108 19 5.344004315003332 1108 20 5.707138128335885 1108 21 5.371330882139841 1108 22 2.247087089428181 1108 23 5.190398959261916 1108 24 5.970069596460693 1108 25 6.365657237706586 1109 1 3.6848747587318122 1109 2 -13.820686350015828 1109 3 -5.541618188378816 1109 4 2.0636915583321596 1109 5 2.12806449886234 1109 6 2.826675466420845 1109 7 3.7295896228454453 1109 8 4.095585045654044 1109 9 3.5858345912464316 1109 10 2.7339445760066035 1109 11 1.4325821328816772 1109 12 1.7649333376372347 1109 13 2.6086631012137396 1109 14 3.452769551303817 1109 15 3.544222749327421 1109 16 3.338686532333167 1109 17 3.9251158854926267 1109 18 3.996927743266511 1109 19 2.6144125240523124 1109 20 1.767770497904614 1109 21 0.9189829397862432 1109 22 3.079907245030398 1109 23 3.5528634013732265 1109 24 4.70146452009536 1109 25 4.9733090261449995 1110 1 2.9617637867925204 1110 2 9.190600331699747 1110 3 6.305384761757907 1110 4 7.597705877736075 1110 5 8.20386018698288 1110 6 3.9232786799134765 1110 7 6.528412550408806 1110 8 7.279767862369344 1110 9 5.567052704076545 1110 10 5.140976843987296 1110 11 2.819330490348321 1110 12 5.7096102449029225 1110 13 9.965077928059522 1110 14 7.737014995338395 1110 15 6.979792887336927 1110 16 6.62971430858158 1110 17 7.113975104784593 1110 18 7.194938465614143 1110 19 6.185754905083346 1110 20 6.321925923770387 1110 21 3.921361860302312 1110 22 6.108681974792368 1110 23 6.421665476103904 1110 24 7.336025509469362 1110 25 7.883726725130117 1111 1 4.8100415069454705 1111 2 9.392788290408093 1111 3 7.3447870432454145 1111 4 7.9865817606917915 1111 5 8.180145908917545 1111 6 7.423882174199505 1111 7 7.304275513061651 1111 8 7.468075167697581 1111 9 5.763745703485867 1111 10 4.944013283194433 1111 11 6.989464045143269 1111 12 6.391075764845006 1111 13 7.887243398844873 1111 14 5.848630964497815 1111 15 5.437033651083297 1111 16 5.234798356339368 1111 17 6.461382756488362 1111 18 6.5032912936071625 1111 19 5.179484716220106 1111 20 4.799442272797986 1111 21 7.5652159303144835 1111 22 6.912751104080268 1111 23 7.07113127401221 1111 24 6.605636225750718 1111 25 7.2978183594290975 1112 1 -2.297174663844192 1112 2 7.566169512568189 1112 3 6.063179371686029 1112 4 -0.15407254028530204 1112 5 4.054426431684433 1112 6 3.0482812789128744 1112 7 5.881553911812457 1112 8 6.167413539797835 1112 9 4.787523715820651 1112 10 3.5618028930979904 1112 11 0.33917139224887904 1112 12 4.1797223137570185 1112 13 8.120995976129493 1112 14 5.8163235322506495 1112 15 3.494341945487644 1112 16 4.027477539455337 1112 17 5.017890799425487 1112 18 5.2700560106302134 1112 19 4.866377496091875 1112 20 4.509753503059535 1112 21 -1.517147499748173 1112 22 2.6635967248023427 1112 23 3.929185715987334 1112 24 5.7419667378042405 1112 25 5.80529594758255 1113 1 -18.61009475346331 1113 2 -13.820686350015828 1113 3 -7.6843169688666215 1113 4 -6.472624295326378 1113 5 -1.6941196474791234 1113 6 -1.098073314066962 1113 7 -20.58595915764236 1113 8 -9.653275929955713 1113 9 -5.539078823387712 1113 10 -4.821615180090959 1113 11 -6.022772745167103 1113 12 -3.9775837355334964 1113 13 -2.5686954353716267 1113 14 -3.124371912110817 1113 15 -3.296488226282335 1113 16 -3.6977890774229323 1113 17 -3.446113382800057 1113 18 -4.22762225673349 1113 19 -3.724480158874517 1113 20 -4.551161209412459 1113 21 -1.8910414504576565 1113 22 -2.799432998872039 1113 23 -3.1589744035048213 1113 24 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2204 3 2.9903032692286153 2204 4 5.396480466578385 2204 5 0.6918529715684976 2204 6 3.607852876409229 2204 7 4.631302747119545 2204 8 4.666922879568098 2204 9 2.986930700421812 2204 10 2.5581860103852314 2204 11 3.9871879691186116 2204 12 4.3894615025617405 2204 13 2.5161652789140874 2204 14 3.81461261169871 2204 15 3.831334392765285 2204 16 3.652710922577068 2204 17 -1.0121300494667231 2204 18 2.4805586956474635 2204 19 3.0358519839826243 2204 20 3.3797768858256347 2204 21 4.920434740018532 2204 22 4.2348324773184345 2204 23 2.978476786971367 2204 24 4.482478602557613 2204 25 4.6266372781775225 2205 1 5.723484038878368 2205 2 9.925999953371507 2205 3 6.713847390485366 2205 4 7.267123569180249 2205 5 7.499098614671098 2205 6 6.790581766934512 2205 7 6.924350120708155 2205 8 6.648337030279929 2205 9 5.409917936553377 2205 10 4.883104702088719 2205 11 7.047087637748948 2205 12 6.257174438251482 2205 13 9.156053901889049 2205 14 6.761877277874458 2205 15 5.5471312140711255 2205 16 5.174157903431265 2205 17 5.802778517052669 2205 18 5.6656961526921386 2205 19 4.135700106221211 2205 20 4.370418761132459 2205 21 -1.8943050734326192 2205 22 2.9547714193901093 2205 23 4.21765357882213 2205 24 5.950024319297917 2205 25 6.180078306650767 2206 1 4.021679559825479 2206 2 8.813315134765167 2206 3 6.07051955674585 2206 4 1.9137145316966375 2206 5 5.521035215178635 2206 6 5.692455564923389 2206 7 5.929259847554373 2206 8 5.878731196993062 2206 9 4.397326818780066 2206 10 3.6938164457442326 2206 11 3.1818550202374993 2206 12 4.454423985327676 2206 13 7.817040570567496 2206 14 0.4294793128335037 2206 15 2.583331224348697 2206 16 3.4369072848636293 2206 17 4.521857515492446 2206 18 -0.5465253005345296 2206 19 1.4883804201900688 2206 20 2.7530335938540595 2206 21 3.8139414745609024 2206 22 -1.339829138441452 2206 23 -2.2029631191692616 2206 24 1.6340207199613666 2206 25 3.398178202453689 2207 1 0.6650932164615 2207 2 -13.820686350015828 2207 3 -6.425916968866622 2207 4 -4.616552114875251 2207 5 0.6766352397389213 2207 6 1.2603748062337914 2207 7 2.374943097996738 2207 8 2.611463919668349 2207 9 0.29639185330401574 2207 10 -0.07507683422629796 2207 11 -1.898032895543043 2207 12 -2.5987747129771073 2207 13 0.05754215861333378 2207 14 -5.913496724140892 2207 15 -1.6897573992146668 2207 16 -0.8782101300545122 2207 17 0.31670766983152276 2207 18 0.35017022446951884 2207 19 -0.2984290310549689 2207 20 -0.5069085778335136 2207 21 -3.837898593314801 2207 22 -4.440864577819408 2207 23 -1.6897954561364017 2207 24 0.05546575794608444 2207 25 0.4968832054958163 2208 1 4.727324136226667 2208 2 6.27808798307718 2208 3 6.039620161630854 2208 4 7.815541818588546 2208 5 3.3919406264934793 2208 6 5.7102975583194855 2208 7 6.3217553340663635 2208 8 6.4585954471171085 2208 9 4.6782437433029855 2208 10 4.274804718971765 2208 11 6.189617737889854 2208 12 5.590060388475155 2208 13 7.972785747036447 2208 14 2.720811649533019 2208 15 4.208986034712616 2208 16 4.422582948532366 2208 17 5.403657922174709 2208 18 0.005016243627000705 2208 19 2.839721138890443 2208 20 3.9828534985904227 2208 21 6.51591745365193 2208 22 5.58956080069537 2208 23 1.3963617176199072 2208 24 2.859183957554695 2208 25 4.15185282131619 2209 1 3.205074613252446 2209 2 5.0968394231800485 2209 3 5.522389363975794 2209 4 0.7472413895042571 2209 5 5.267876228809538 2209 6 5.605014756625234 2209 7 6.1739819321956375 2209 8 4.752902567835157 2209 9 4.4695644755813575 2209 10 3.475888207243358 2209 11 -0.15836155223632176 2209 12 2.943477825585795 2209 13 2.62144418171232 2209 14 3.8796976018803484 2209 15 4.280562731007798 2209 16 3.569592365876037 2209 17 1.7571231415003865 2209 18 3.6082587447392633 2209 19 3.993579340389104 2209 20 3.9109194975094823 2209 21 6.2028966938722405 2209 22 4.996008532792171 2209 23 0.5880512224156469 2209 24 -2.784879951688829 2209 25 0.12116814464782166 2210 1 -2.272717952856791 2210 2 -13.820686350015828 2210 3 -7.557910903665713 2210 4 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-12.384157860896536 2211 17 -8.599838860994392 2211 18 -6.889285913963789 2211 19 -9.67865062559219 2211 20 -7.87034835554865 2211 21 -5.654031504015425 2211 22 -6.8609185382752536 2211 23 -8.806072184683092 2211 24 -21.808444016490007 2211 25 -21.618154388489145 2212 1 -1.7780758260185152 2212 2 -13.820686350015828 2212 3 -8.15083810451331 2212 4 -19.365424295326378 2212 5 -20.005319647479123 2212 6 -20.73887331406696 2212 7 -10.344634236506714 2212 8 -9.836557948883158 2212 9 -5.875601220864056 2212 10 -6.197008240027866 2212 11 -5.712458549583509 2212 12 -3.4715635462590484 2212 13 -6.803552217706013 2212 14 -4.709344782773275 2212 15 -4.518933652149844 2212 16 -4.824615575845645 2212 17 -4.937044613083966 2212 18 -4.16464560058207 2212 19 -4.773359654142656 2212 20 -4.857614206257885 2212 21 -2.744410535631161 2212 22 -3.971986942089707 2212 23 -3.983834971328168 2212 24 -2.9740591584458436 2212 25 -3.523517164514381 2213 1 -18.61009475346331 2213 2 -13.820686350015828 2213 3 -8.767212515425326 2213 4 -6.220485024071317 2213 5 -20.005319647479123 2213 6 -11.006079791799754 2213 7 -20.58595915764236 2213 8 -10.746662164773525 2213 9 -8.902567082497026 2213 10 -7.81041518009096 2213 11 -7.165470720875607 2213 12 -5.703112480472768 2213 13 -19.057495435371628 2213 14 -12.471102276483286 2213 15 -7.373910088630513 2213 16 -6.491165595641554 2213 17 -5.2735757309781945 2213 18 -5.328198774952114 2213 19 -6.217746555635653 2213 20 -6.207718294432702 2213 21 -4.149292462603405 2213 22 -5.094305873365968 2213 23 -4.9833177233428785 2213 24 -3.9112780245871726 2213 25 -4.661069368246231 2214 1 -18.61009475346331 2214 2 -13.820686350015828 2214 3 -12.095219294448018 2214 4 -19.365424295326378 2214 5 -20.005319647479123 2214 6 -20.73887331406696 2214 7 -16.367354506479572 2214 8 -14.663080581118503 2214 9 -13.636995102457481 2214 10 -13.581577970788633 2214 11 -14.333507628888034 2214 12 -12.03587210762652 2214 13 -19.057495435371628 2214 14 -16.59240912141314 2214 15 -23.522088226282335 2214 16 -18.017696054167118 2214 17 -23.251713382800055 2214 18 -17.471473419524187 2214 19 -14.77701969375824 2214 20 -13.478193767551994 2214 21 -11.042948427201845 2214 22 -12.023581836081343 2214 23 -11.900444170946683 2214 24 -10.82332773742024 2214 25 -10.582805551279844 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 141867.0 3 7.0 4 670.0 5 2720.0 6 3804.0 7 3653.0 8 3003.0 9 2808.0 10 2655.0 11 2500.0 12 2343.0 13 2486.0 14 3780.0 15 7554.0 16 11298.0 17 11888.0 18 12080.0 19 14258.0 20 17062.0 21 20132.0 22 20744.0 23 22525.0 24 23529.0 25 24274.0 26 20404.0 27 16288.0 28 17048.0 29 19860.0 30 19081.0 31 21894.0 32 28212.0 33 35202.0 34 42875.0 35 49504.0 36 49083.0 37 51065.0 38 37763.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66492306167827 19.19186611275679 12.658301966960586 25.484908858604342 2 13.651078067371781 20.02353563454982 42.62574035766784 23.69964594041056 3 18.785022184748502 24.233071170884763 29.848093217410536 27.1338134269562 4 11.064563155146551 17.507145681352412 39.22820604915505 32.200085114345995 5 12.128436827478946 37.53910794697595 37.45320609812704 12.87924912741806 6 31.459853012301863 37.86008858320275 18.3731571230446 12.306901281450774 7 27.252703186124606 31.662762385035254 22.98025549003543 18.104278938804715 8 23.964809152553848 37.93410319188546 21.190384854057836 16.91070280150286 9 25.423270257765505 16.40683610546925 20.61914956464004 37.5507440721252 10 13.368752449241134 30.464058379317283 35.3156740841162 20.851515087325375 11 32.84821273054518 23.264223922233253 24.760365720478745 19.127197626742827 12 22.285783836416748 25.657669071434896 32.300472470567406 19.75607462158095 13 27.436459423128863 21.910672209166236 25.98279507135606 24.670073296348843 14 19.68881758638063 22.75184959295696 28.844634191615175 28.714698629047223 15 22.058475569752307 31.213365383581237 24.213532087890812 22.514626958775636 16 21.114384089292702 29.819131730126742 27.705932097429287 21.360552083151273 17 20.78285807975874 29.164057229328556 28.870411534026612 21.182673156886093 18 20.425504854793388 28.71329758975969 30.459671895720973 20.401525659725948 19 21.605500578604094 28.235879809232774 28.352262646537774 21.806356965625355 20 21.833533431178342 28.451949677464967 29.146902367526277 20.56761452383041 21 21.42049245302841 27.235815063381462 27.843775027526014 23.499917456064114 22 21.09351273859587 28.711472892273292 29.208789372545745 20.986224996585094 23 21.22954022943436 28.371491793872426 28.757847023718497 21.641120952974713 24 21.01673855089432 28.20602220519575 29.370804959868224 21.4064342840417 25 21.50613527403527 28.1170433573307 28.789910650333823 21.58691071830021 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 870.0 1 870.0 2 1771.0 3 2672.0 4 2672.0 5 2672.0 6 3835.0 7 4998.0 8 4998.0 9 4998.0 10 6080.5 11 7163.0 12 7163.0 13 7163.0 14 9118.5 15 11074.0 16 11074.0 17 11074.0 18 15449.0 19 19824.0 20 19824.0 21 19824.0 22 27198.0 23 34572.0 24 34572.0 25 34572.0 26 43624.0 27 52676.0 28 52676.0 29 52676.0 30 62339.5 31 72003.0 32 72003.0 33 72003.0 34 78853.5 35 85704.0 36 85704.0 37 85704.0 38 89762.5 39 93821.0 40 93821.0 41 93821.0 42 94473.5 43 95126.0 44 95126.0 45 95126.0 46 92383.5 47 89641.0 48 89641.0 49 89641.0 50 82193.0 51 74745.0 52 74745.0 53 74745.0 54 64045.0 55 53345.0 56 53345.0 57 53345.0 58 43512.0 59 33679.0 60 33679.0 61 33679.0 62 26418.5 63 19158.0 64 19158.0 65 19158.0 66 14099.0 67 9040.0 68 9040.0 69 9040.0 70 6444.5 71 3849.0 72 3849.0 73 3849.0 74 2606.0 75 1363.0 76 1363.0 77 1363.0 78 888.5 79 414.0 80 414.0 81 414.0 82 274.5 83 135.0 84 135.0 85 135.0 86 87.0 87 39.0 88 39.0 89 39.0 90 23.0 91 7.0 92 7.0 93 7.0 94 4.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.038715582196028 2 36.315589507506665 3 5.542230966982148 4 13.484585184595238 5 13.36577366536148 6 14.42580742872288 7 9.48703452976098 8 7.425197703673627 9 0.1051024977837082 10 1.3817388000558806 11 5.5521536872698025 12 5.602550661362363 13 15.887450239516191 14 1.527446113753543 15 2.8837253025450473 16 2.8877727279255376 17 2.8495180299744494 18 1.4489782862156442 19 1.3908781476892464 20 0.2775750438362281 21 4.305416107969641 22 1.550033358618862 23 0.10706092941942948 24 9.004215850501163 25 0.10901936105515075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 765919.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.51704061182608 #Duplication Level Percentage of deduplicated Percentage of total 1 97.64164336024525 93.26440814245984 2 1.8699238681063843 3.5721918810188082 3 0.2412322306087236 0.6912536630380454 4 0.0895719049401006 0.3422257312736885 5 0.047910823790508664 0.22881500508720298 6 0.02245381725254204 0.12868333046409472 7 0.016761168910217682 0.11206840760492572 8 0.011725339286494493 0.08959757670524274 9 0.009520929626081974 0.08184699195811289 >10 0.043012675800807046 0.7532615935957754 >50 0.004162804249252705 0.26825355522509153 >100 0.0019423387051779825 0.3998094882810732 >500 1.387384784446504E-4 0.0675846332881052 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 1.3056210904808472E-4 0.0 0.0 0.0 10 0.0 1.3056210904808472E-4 0.0 0.0 0.0 11 0.0 1.3056210904808472E-4 0.0 1.3056210904808472E-4 0.0 12 0.0 2.6112421809616944E-4 0.0 1.3056210904808472E-4 0.0 13 0.0 2.6112421809616944E-4 0.0 1.3056210904808472E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 280 0.0 17.162361 1 TCCTACA 325 0.0 15.985421 2 CTAGGGC 50 5.134877E-4 15.320634 3 GTCTAGG 110 9.8301825E-8 14.562003 1 GCCTAGG 65 8.318727E-4 14.37531 1 CTATAGG 85 2.7654844E-5 14.133708 1 GTAAGAC 70 2.8881714E-5 14.069969 3 GTGTAGG 95 5.0513518E-6 14.051054 1 GTCATAC 80 2.6206876E-4 13.348503 1 TAGGACT 105 5.5621058E-8 13.280208 4 CTAGATA 65 2.8799096E-4 13.201627 4 TGTAGGA 250 0.0 13.124871 2 GACAGTC 70 5.654445E-5 13.099924 7 TATAGGA 105 1.1088838E-5 13.020706 2 GTAGGAC 195 0.0 12.907495 3 CTAAAGT 125 3.0031515E-9 12.871586 4 GTGTAGC 95 7.860137E-5 12.645949 1 GGACGTG 185 0.0 12.392191 6 TAGGACG 235 0.0 12.323859 4 GACGTGG 100 8.2631595E-7 12.226597 7 >>END_MODULE