FastQCFastQC Report
Thu 26 May 2016
SRR1926645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765919
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16320.21307736196647425No Hit
GGTATCAACGCAGAGTACTTTTTTT9620.12560074890425751No Hit
CACACACACACACACACACACACAC8180.1067998052013333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCT307.705302E-419.0045837
TAGGACC2150.013.3051894
AGGCGTG500.001495608413.3032087
GAGTAAG1051.8833816E-812.7155181
GGCGAGG2150.011.89649619
CTAGGAC1052.580473E-711.8072673
GACGTGA1701.8189894E-1211.7381267
GTAGGAC4400.011.7040573
ATAACAC1156.684786E-811.6098223
CTGTAGG5350.011.5865711
TGTAGGA5200.011.5578032
ATTAGAC751.9984177E-411.4439673
TTACTCA752.0008598E-411.4424634
GTCCTAC4200.011.3531411
TGGCGAG4600.011.32657618
TAGGACT1701.0913936E-1111.2181014
CTAATAC600.005705144311.1260793
GTCTTAG951.3008497E-511.0424241
AGGACGT3300.010.9801425
CGTGAAA1951.8189894E-1210.7212369
Produced by FastQC (version 0.11.5)