##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 765919 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.125698670486045 31.0 28.0 31.0 16.0 34.0 2 28.62086721964072 31.0 28.0 33.0 22.0 34.0 3 28.97694664840538 31.0 28.0 33.0 23.0 34.0 4 32.70903320063871 35.0 33.0 37.0 26.0 37.0 5 32.33151547356835 35.0 32.0 37.0 25.0 37.0 6 32.42232533727457 35.0 33.0 37.0 25.0 37.0 7 32.23893257642127 35.0 33.0 37.0 22.0 37.0 8 32.19477255427793 35.0 33.0 37.0 22.0 37.0 9 33.39172941264024 37.0 33.0 39.0 19.0 39.0 10 33.289488836286864 37.0 33.0 39.0 18.0 39.0 11 33.52662357246654 37.0 33.0 39.0 22.0 39.0 12 33.322634638910905 37.0 33.0 39.0 19.0 39.0 13 33.468280588417315 37.0 33.0 39.0 22.0 39.0 14 34.08188855479496 38.0 33.0 40.0 17.0 41.0 15 34.21027027662194 38.0 33.0 40.0 18.0 41.0 16 34.175287465123596 38.0 33.0 40.0 18.0 41.0 17 34.08948465829938 38.0 33.0 40.0 17.0 41.0 18 34.049915199910174 38.0 33.0 40.0 17.0 41.0 19 34.104816566764896 39.0 34.0 40.0 18.0 41.0 20 34.07920419783293 39.0 34.0 40.0 18.0 41.0 21 34.005362185818605 39.0 34.0 40.0 18.0 41.0 22 33.92262497731483 39.0 34.0 40.0 16.0 41.0 23 33.58825933290596 39.0 33.0 40.0 10.0 41.0 24 33.655128022676024 39.0 33.0 40.0 2.0 41.0 25 33.452259312016025 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5394969774420204 1101 2 0.7771778837859102 1101 3 0.7439072324843821 1101 4 0.5258103711507687 1101 5 0.4567139740663677 1101 6 0.1692804097183398 1101 7 0.35595883701194 1101 8 0.3429638734352807 1101 9 0.7469696305838198 1101 10 0.8648656203979712 1101 11 0.5421864965516434 1101 12 0.5159245135877271 1101 13 0.6205507534000816 1101 14 0.7782432939906272 1101 15 0.6004855708901928 1101 16 0.7361680369609616 1101 17 0.49996563831367524 1101 18 0.7607983616921814 1101 19 0.775292876311461 1101 20 0.4988957528496556 1101 21 0.7569904360551902 1101 22 0.5782283147662568 1101 23 1.0257557206764645 1101 24 1.0289571307203502 1101 25 0.931214584658612 1102 1 0.627010917877513 1102 2 0.6543934989019142 1102 3 0.6488608862496719 1102 4 0.5014443181679766 1102 5 0.7933880603965093 1102 6 0.3491417366649756 1102 7 0.6278247528756964 1102 8 0.6403590687841998 1102 9 0.9943232331579992 1102 10 1.0115144311188544 1102 11 0.8770209024605577 1102 12 0.8238355964624731 1102 13 0.8972032663349765 1102 14 1.0539348310036232 1102 15 0.8281271691496954 1102 16 0.6807445219601362 1102 17 0.899376254983693 1102 18 0.9125228621218255 1102 19 1.1950713833593767 1102 20 1.140839095263317 1102 21 1.1341226370899804 1102 22 1.3097355272276303 1102 23 1.369809096547172 1102 24 1.0607093291955891 1102 25 1.395974991993569 1103 1 0.381195595519376 1103 2 0.12713676203076574 1103 3 0.20114176549182972 1103 4 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0.7152320865952788 2202 20 0.6169474561995116 2202 21 0.776098395106942 2202 22 0.6604525948940676 2202 23 1.0775243988439698 2202 24 0.9217820187453256 2202 25 0.7974691862934264 2203 1 -0.36663338410104274 2203 2 -0.2816465327844675 2203 3 -0.44705488693481144 2203 4 -0.5992981010501808 2203 5 -0.41416002184069356 2203 6 -0.5790803258972019 2203 7 -0.3871894323053944 2203 8 -0.1615780374388578 2203 9 -0.06150558670861983 2203 10 -0.14564632198530347 2203 11 -0.1662692305485436 2203 12 -0.3517405613799056 2203 13 -0.3247050089680741 2203 14 0.07020173347589775 2203 15 0.013858627091551057 2203 16 -0.25266802731955096 2203 17 -0.12650018656230344 2203 18 -0.038506024065128486 2203 19 -0.1593140482754194 2203 20 -0.08711400152751736 2203 21 -0.2734618292156057 2203 22 -0.12547319757985065 2203 23 0.06405626872211201 2203 24 -0.16459556008631182 2203 25 -0.1851476637670899 2204 1 -0.029723161296324463 2204 2 -0.10224914309464594 2204 3 -0.3618784998662221 2204 4 -0.5914002744883646 2204 5 -0.294723313671188 2204 6 -0.2350163241497114 2204 7 -0.04649753628093123 2204 8 -0.1366148985135638 2204 9 0.3007363857684453 2204 10 0.0325360174642384 2204 11 -0.08972106541092728 2204 12 0.08032584316356406 2204 13 -0.2700550526552732 2204 14 0.1510913140787835 2204 15 -0.04118041834313857 2204 16 0.13961572104217623 2204 17 -0.005923515526923495 2204 18 -0.05983083839452519 2204 19 -0.09344876867734087 2204 20 -0.04849861271144107 2204 21 0.09699197157076611 2204 22 0.2514039867801259 2204 23 0.3042880839252575 2204 24 0.18032170727935437 2204 25 0.24842048826436525 2205 1 0.43999333354903314 2205 2 0.4066103929878295 2205 3 0.4693224083321077 2205 4 0.30016053798095044 2205 5 0.160257970079428 2205 6 0.24470262528573272 2205 7 -0.0047541636741570414 2205 8 0.39875421047023707 2205 9 0.7012717932303971 2205 10 0.6292238549703697 2205 11 0.35856378976825454 2205 12 0.4839831823879095 2205 13 0.3580898908891541 2205 14 0.6887539306718153 2205 15 0.5756197166593182 2205 16 0.5981638310078168 2205 17 0.6190151197209914 2205 18 0.6503127551253556 2205 19 0.6676812632323319 2205 20 0.8177492395222359 2205 21 0.7734309750071873 2205 22 0.5714288395047262 2205 23 0.8399026519173987 2205 24 0.5522764814570635 2205 25 0.6188488142339281 2206 1 0.21153386489945447 2206 2 0.6273135756454131 2206 3 0.6666670776075279 2206 4 0.44642021283797106 2206 5 0.7700022006993734 2206 6 0.4291779170211427 2206 7 0.5412669403337702 2206 8 0.5621386145843275 2206 9 0.7598933085598318 2206 10 0.6868952416661642 2206 11 0.47382292122603786 2206 12 0.43680585164802466 2206 13 0.5805686879322778 2206 14 0.8087134108016585 2206 15 0.45319033942544706 2206 16 0.7183157422533384 2206 17 0.5962612336511484 2206 18 0.4854616104335534 2206 19 0.6217301341755572 2206 20 0.5359797916792743 2206 21 0.648684224195641 2206 22 0.7175605914041583 2206 23 0.9397262931733152 2206 24 0.994052948662322 2206 25 0.8909660922520644 2207 1 -1.033388574830159 2207 2 0.07366721028881429 2207 3 0.41724117431924057 2207 4 0.6208256027046204 2207 5 0.3602657214165319 2207 6 0.46764658311845864 2207 7 0.4702787795085399 2207 8 0.5400533345691443 2207 9 0.8000393306305966 2207 10 0.4473464811233825 2207 11 0.5412783080226546 2207 12 0.39674188326381454 2207 13 0.3991022155151569 2207 14 -4.81819126737485 2207 15 -1.9454019095712027 2207 16 -0.6939995671783734 2207 17 -0.40792852805824964 2207 18 -0.021766928620088777 2207 19 0.18804402580887114 2207 20 0.3895731291432014 2207 21 0.421349740994323 2207 22 0.32614552813350883 2207 23 0.4867207280355288 2207 24 0.15542352432196083 2207 25 0.07244931031950586 2208 1 0.49775778421814465 2208 2 0.7886430059423297 2208 3 0.82542945323571 2208 4 0.7346272300606813 2208 5 0.8254320619253406 2208 6 0.8862993794929537 2208 7 1.0529752467039302 2208 8 1.0199585771275963 2208 9 1.3614977712527008 2208 10 1.3582126877358078 2208 11 1.2361696459565295 2208 12 1.0732791167585063 2208 13 0.7969907725241185 2208 14 1.300605725106351 2208 15 1.2110302667600337 2208 16 1.0148581936620857 2208 17 1.1693090865261553 2208 18 1.17974713050031 2208 19 1.0125058653392074 2208 20 1.1037661459317434 2208 21 1.2403630276355813 2208 22 1.2569931952465012 2208 23 1.4343569418577573 2208 24 1.3329598308416806 2208 25 1.422629555452886 2209 1 -0.4186222776438946 2209 2 -0.06761641113588368 2209 3 0.2469365467709892 2209 4 0.44301768083809634 2209 5 0.37380730734010115 2209 6 0.3198130818394844 2209 7 0.6020205049561795 2209 8 0.6726864637839327 2209 9 0.7955280524351096 2209 10 0.6159690685197106 2209 11 0.5691676189287875 2209 12 0.6879596183231129 2209 13 0.65632258602907 2209 14 0.8691663018058335 2209 15 0.9408627210775791 2209 16 0.923642476074086 2209 17 0.7169532640508933 2209 18 0.9625366137409657 2209 19 0.7729831778125913 2209 20 0.7949215517460999 2209 21 0.8559052018113107 2209 22 0.7385864241880853 2209 23 1.01074212172594 2209 24 0.900582814299483 2209 25 0.9524276065756041 2210 1 -0.21909145979085665 2210 2 -0.6438272460947516 2210 3 0.1755811105890288 2210 4 0.1640698011461339 2210 5 0.5790114105065811 2210 6 0.564644764698258 2210 7 0.6247259284650895 2210 8 0.7323452850670691 2210 9 1.010842684732303 2210 10 1.101389473365174 2210 11 0.67263836092215 2210 12 0.3796778053282637 2210 13 0.44772243931194566 2210 14 0.9081233820234758 2210 15 0.815292852352556 2210 16 0.831118573130702 2210 17 0.7861989437676371 2210 18 0.8020030725769871 2210 19 0.8255177328209058 2210 20 0.9754310643527191 2210 21 0.9634977569781782 2210 22 0.8736370773249149 2210 23 0.9928446428262987 2210 24 0.9194170942969322 2210 25 0.8742169971636002 2211 1 -1.9567848620286448 2211 2 -1.153684067676565 2211 3 -0.1567479300407406 2211 4 -0.30089664476562206 2211 5 0.18784365630731514 2211 6 -0.06433965639003247 2211 7 0.05562086463719851 2211 8 0.0885377821072666 2211 9 0.25382950614589816 2211 10 0.25451781419976527 2211 11 -0.10412268489575638 2211 12 0.008505607800863402 2211 13 -0.03318604147008131 2211 14 0.2599340382380717 2211 15 -0.2209672575241015 2211 16 -0.06080008032044759 2211 17 -0.09831977012669313 2211 18 0.09910046557311603 2211 19 0.06472482497588317 2211 20 0.0826365067916015 2211 21 0.033796312664506445 2211 22 -0.09819535467795504 2211 23 0.12254747930543886 2211 24 -0.080211661576989 2211 25 0.21352368715677272 2212 1 0.7704965322593296 2212 2 -0.1626578294398442 2212 3 0.08941806765668758 2212 4 0.07848433945184752 2212 5 0.30094431063405125 2212 6 0.061016047789600236 2212 7 0.16811208517017207 2212 8 0.0719038599361923 2212 9 0.2655595981764378 2212 10 -0.123894713345436 2212 11 -0.13076338928053843 2212 12 0.12118508907012426 2212 13 0.059846742447625445 2212 14 0.1523822484335966 2212 15 0.2491131636006827 2212 16 -0.37423248385492514 2212 17 -0.06792591901197653 2212 18 -0.1464971295166535 2212 19 -0.14767974734640887 2212 20 0.053703486582534765 2212 21 0.03762307416953092 2212 22 0.2089791895741797 2212 23 0.41888315302544754 2212 24 0.3082673847617059 2212 25 -0.2194235105338933 2213 1 0.42450616201194435 2213 2 0.17829416228418538 2213 3 -0.3762232559872949 2213 4 -1.065062193709494 2213 5 -1.8507811264724232 2213 6 -0.47792889403596917 2213 7 -0.18235279943882432 2213 8 -0.11801491297278943 2213 9 -0.8894921904798707 2213 10 -0.6038970122253744 2213 11 -0.7999015647361674 2213 12 -0.9805282150589179 2213 13 -0.7877688081979741 2213 14 -7.612754598780288 2213 15 -3.415268884701341 2213 16 -2.304236698468138 2213 17 -1.473284224992895 2213 18 -5.4877588314541015 2213 19 -3.263951347239132 2213 20 -5.18179760538543 2213 21 -2.351351242232976 2213 22 -2.1573709553829694 2213 23 -4.812296044665452 2213 24 -2.5515169036711 2213 25 -2.0842308516238184 2214 1 0.5855343137867308 2214 2 0.1923418037492084 2214 3 -1.6131312684155006 2214 4 -1.7551177440343224 2214 5 -0.18552550082512198 2214 6 -0.1283911857222506 2214 7 -0.17373590837395625 2214 8 -0.3885355795766863 2214 9 -5.172844040588146 2214 10 -1.4690095251714226 2214 11 -0.9434323057210037 2214 12 -1.0976697271575873 2214 13 -1.4013908776829567 2214 14 -2.669920590683123 2214 15 -2.3803380872246436 2214 16 -1.363609638869228 2214 17 -1.4792347004660087 2214 18 -1.1311182138098133 2214 19 -4.08230743423659 2214 20 -2.1612609327557344 2214 21 -3.682178847919822 2214 22 -5.765520847106913 2214 23 -6.195118754391469 2214 24 -5.488769516419428 2214 25 -3.3760451817245603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28682.0 3 14.0 4 490.0 5 3427.0 6 4159.0 7 3432.0 8 2591.0 9 2149.0 10 1913.0 11 1710.0 12 1596.0 13 1594.0 14 1549.0 15 1660.0 16 1707.0 17 1653.0 18 1944.0 19 2269.0 20 2979.0 21 3799.0 22 4936.0 23 6064.0 24 7388.0 25 8553.0 26 9675.0 27 10748.0 28 12243.0 29 15069.0 30 17956.0 31 22819.0 32 29129.0 33 37190.0 34 48239.0 35 62943.0 36 85092.0 37 122827.0 38 195731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.563373655826155 19.887173683790984 9.533523163109429 30.01592949727343 2 13.306079801475871 21.47613139162803 39.69607523019656 25.521713576699533 3 18.086940394998393 24.538249960165814 29.257621389782074 28.117188255053716 4 11.6478226287792 17.838601697073546 37.812585300997426 32.70099037314982 5 12.758105063828399 38.72042835221522 35.41179927519671 13.109667308759674 6 31.37408561596264 38.83357227663606 17.46116406886809 12.331178038533212 7 27.979589834167403 32.26284630229287 21.348902626500507 18.40866123703922 8 24.73551914141368 38.65300242203621 19.609333802649193 17.00214463390092 9 25.472817801739083 16.479825175511486 20.0977499198503 37.94960710289913 10 14.034398149901344 30.780350406178137 34.10551360216872 21.079737841751797 11 33.98586223645109 23.890313516788268 23.52120142265489 18.60262282410575 12 22.0993724914674 26.244375794831083 31.54790660751078 20.10834510619074 13 28.033120995823317 22.600039952005368 25.85508389268317 23.511755159488143 14 20.027830949845495 23.128956600704267 27.982386797938158 28.860825651512084 15 22.401488444966706 31.250816033424726 23.50946598772686 22.83822953388171 16 21.557972197160698 30.007977615672164 27.205236240571463 21.228813946595672 17 21.029678666092156 29.737814511601186 27.775340462480578 21.45716635982608 18 20.801637850217137 29.32749431374546 29.13268623626094 20.738181599776468 19 22.254602428515472 28.40057448753101 28.09844627235932 21.246376811594203 20 22.16665992085201 28.542499285161256 28.64068352946245 20.65015726452428 21 22.00611291794563 27.939899649962985 27.889763800088517 22.164223632002862 22 21.634272031376685 28.858926335552454 28.09866317456546 21.408138458505405 23 21.54653429409637 28.47180968222488 27.99408292521794 21.987573098460803 24 21.444257594827633 28.34592827411883 28.84572198517853 21.364092145875006 25 21.749558373666144 28.212643895764433 28.2489401620798 21.788857568489618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 275.0 1 275.0 2 311.5 3 348.0 4 348.0 5 348.0 6 502.5 7 657.0 8 657.0 9 657.0 10 1218.5 11 1780.0 12 1780.0 13 1780.0 14 2959.0 15 4138.0 16 4138.0 17 4138.0 18 7094.5 19 10051.0 20 10051.0 21 10051.0 22 15558.5 23 21066.0 24 21066.0 25 21066.0 26 28929.5 27 36793.0 28 36793.0 29 36793.0 30 47362.0 31 57931.0 32 57931.0 33 57931.0 34 67139.5 35 76348.0 36 76348.0 37 76348.0 38 83428.5 39 90509.0 40 90509.0 41 90509.0 42 95103.5 43 99698.0 44 99698.0 45 99698.0 46 101519.5 47 103341.0 48 103341.0 49 103341.0 50 98759.0 51 94177.0 52 94177.0 53 94177.0 54 82026.0 55 69875.0 56 69875.0 57 69875.0 58 58634.0 59 47393.0 60 47393.0 61 47393.0 62 37637.5 63 27882.0 64 27882.0 65 27882.0 66 21054.0 67 14226.0 68 14226.0 69 14226.0 70 10209.0 71 6192.0 72 6192.0 73 6192.0 74 4187.5 75 2183.0 76 2183.0 77 2183.0 78 1482.5 79 782.0 80 782.0 81 782.0 82 494.0 83 206.0 84 206.0 85 206.0 86 130.5 87 55.0 88 55.0 89 55.0 90 32.5 91 10.0 92 10.0 93 10.0 94 6.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08773773728031294 2 0.03512120733393479 3 0.03198771671678076 4 0.03237940304392501 5 0.025329049155328433 6 0.37040470336941633 7 0.20811600182264706 8 0.22012771585507082 9 0.22508907599889805 10 0.015928577303866334 11 0.0035251769442982872 12 0.004308549598586796 13 0.0 14 0.07624827168408148 15 0.0024806800719136095 16 0.0033946148352502026 17 0.006397543343356151 18 0.004830798034779134 19 0.0024806800719136095 20 0.0015667453085770166 21 0.0 22 0.0 23 0.0 24 3.9168632714425415E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 765919.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.17731599325614 #Duplication Level Percentage of deduplicated Percentage of total 1 93.36617616069316 82.32778818403459 2 5.298359509271601 9.34390241389831 3 0.7341642751244398 1.942099058358227 4 0.22276112627609437 0.7856991289064321 5 0.11263525464498282 0.49659372204057606 6 0.05597014041772025 0.29611780546801414 7 0.04784690207662549 0.29533079825962916 8 0.02994360408701665 0.21122773116462584 9 0.019159325098814965 0.15204760771101575 >10 0.09737229408163785 1.5713097160861371 >50 0.007434491828054265 0.4491749452016449 >100 0.006690204363744567 1.0772016930346195 >500 0.0013380408325330698 0.8375613357682485 >1k 1.4867120361478555E-4 0.2139458600678951 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1632 0.21307736196647425 No Hit GGTATCAACGCAGAGTACTTTTTTT 962 0.12560074890425751 No Hit CACACACACACACACACACACACAC 818 0.1067998052013333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.3056210904808472E-4 0.0 0.0 1.3056210904808472E-4 0.0 11 1.3056210904808472E-4 0.0 0.0 1.3056210904808472E-4 0.0 12 1.3056210904808472E-4 1.3056210904808472E-4 0.0 1.3056210904808472E-4 0.0 13 1.3056210904808472E-4 1.3056210904808472E-4 0.0 2.6112421809616944E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCGCT 30 7.705302E-4 19.004583 7 TAGGACC 215 0.0 13.305189 4 AGGCGTG 50 0.0014956084 13.303208 7 GAGTAAG 105 1.8833816E-8 12.715518 1 GGCGAGG 215 0.0 11.896496 19 CTAGGAC 105 2.580473E-7 11.807267 3 GACGTGA 170 1.8189894E-12 11.738126 7 GTAGGAC 440 0.0 11.704057 3 ATAACAC 115 6.684786E-8 11.609822 3 CTGTAGG 535 0.0 11.586571 1 TGTAGGA 520 0.0 11.557803 2 ATTAGAC 75 1.9984177E-4 11.443967 3 TTACTCA 75 2.0008598E-4 11.442463 4 GTCCTAC 420 0.0 11.353141 1 TGGCGAG 460 0.0 11.326576 18 TAGGACT 170 1.0913936E-11 11.218101 4 CTAATAC 60 0.0057051443 11.126079 3 GTCTTAG 95 1.3008497E-5 11.042424 1 AGGACGT 330 0.0 10.980142 5 CGTGAAA 195 1.8189894E-12 10.721236 9 >>END_MODULE