FastQCFastQC Report
Thu 26 May 2016
SRR1926644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785271
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG405.4238824E-420.0193541
CTAGTAC300.0075660118.2659233
TAGGACG1200.016.1156524
GATATAC1055.570837E-815.2528411
TATTAAG656.844743E-414.7501882
GTCCTAG751.5183269E-414.2359841
GTGTAAA852.7701963E-514.1313091
ACTGTTC1550.013.8506458
CTACACC703.4351484E-513.81341654
GTAGGAC1800.013.395013
GTCTATA904.7429356E-513.3462351
TCTACAC1451.10958354E-1012.84913353
TATTAGA1409.770338E-812.7182742
TAGGACC1101.0003714E-712.6971814
CTATTAG854.3801733E-412.5611641
AGGACGT1101.4656143E-712.3307765
TGTAGGA2350.012.2395172
GTCCAGG1204.1464154E-612.234051
TATTATG905.6981307E-412.1747592
TATGGCG1500.012.04718516
Produced by FastQC (version 0.11.5)