##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926644_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 785271 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.636228766884297 27.0 2.0 31.0 2.0 31.0 2 16.002259092720855 16.0 2.0 30.0 2.0 31.0 3 19.37956832736724 22.0 12.0 30.0 2.0 31.0 4 21.424915729728973 27.0 2.0 35.0 2.0 35.0 5 22.13111906590209 28.0 2.0 35.0 2.0 37.0 6 22.878743771258584 30.0 2.0 35.0 2.0 37.0 7 22.606673364991195 30.0 2.0 35.0 2.0 37.0 8 22.570982501582257 30.0 2.0 35.0 2.0 37.0 9 25.280967971566504 31.0 17.0 37.0 2.0 39.0 10 26.017067993087736 31.0 17.0 37.0 2.0 39.0 11 23.235668960142423 30.0 7.0 37.0 2.0 39.0 12 24.350752797441903 30.0 7.0 37.0 2.0 39.0 13 21.162830411412113 27.0 2.0 35.0 2.0 39.0 14 24.352622215770097 30.0 8.0 38.0 2.0 40.0 15 25.356433638832964 31.0 8.0 38.0 2.0 40.0 16 25.732330622167378 31.0 7.0 38.0 2.0 40.0 17 25.05534395132381 31.0 2.0 38.0 2.0 40.0 18 25.114756561747473 31.0 2.0 38.0 2.0 40.0 19 25.50406165514835 31.0 2.0 38.0 2.0 40.0 20 25.72946027549725 31.0 2.0 39.0 2.0 40.0 21 23.664219103978116 30.0 2.0 37.0 2.0 40.0 22 24.541423279351967 30.0 2.0 38.0 2.0 40.0 23 24.534213029641997 31.0 2.0 38.0 2.0 40.0 24 23.625985169451056 31.0 2.0 38.0 2.0 40.0 25 23.427294526348227 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.825056801258043 1101 2 10.113329039500426 1101 3 7.195355922270377 1101 4 8.710168587338007 1101 5 8.16916930165057 1101 6 7.735050295730684 1101 7 7.504758873680263 1101 8 5.765521373378132 1101 9 6.067250080210673 1101 10 5.485621227216583 1101 11 8.298278209596138 1101 12 6.048106500735944 1101 13 10.2924683137894 1101 14 7.768499549289558 1101 15 6.951186784069463 1101 16 6.682847639547404 1101 17 2.6144129388620634 1101 18 5.094427298521715 1101 19 5.89132857025983 1101 20 3.6816578078241484 1101 21 1.1707918019501626 1101 22 4.988385659281263 1101 23 6.026325665088795 1101 24 7.398176762546939 1101 25 7.87356637963634 1102 1 5.832423803510963 1102 2 10.569494943569362 1102 3 6.955673699258796 1102 4 8.185368887511181 1102 5 8.211503342704873 1102 6 7.147996020722967 1102 7 7.101283309517438 1102 8 7.027390027070094 1102 9 6.1299020388244045 1102 10 5.910254891053988 1102 11 8.376230241360858 1102 12 5.75953097706838 1102 13 9.887838445369272 1102 14 7.710268548948406 1102 15 5.4907545498392345 1102 16 5.982002753709988 1102 17 6.994722400534567 1102 18 6.950673976855775 1102 19 5.5393684967520045 1102 20 5.829294673977298 1102 21 4.741818278499945 1102 22 5.930894278717155 1102 23 6.325848967166706 1102 24 7.745630453860571 1102 25 7.549819989202522 1103 1 5.060359569381728 1103 2 9.026454737686004 1103 3 6.170803755042439 1103 4 7.600627185307843 1103 5 7.162902447838849 1103 6 6.618043244022331 1103 7 6.659104522312706 1103 8 6.2521143040228075 1103 9 5.530553364671697 1103 10 4.7138183656837676 1103 11 5.404591413943173 1103 12 4.506289439926327 1103 13 8.395950921990124 1103 14 6.655997377459247 1103 15 5.753994395578992 1103 16 5.629109340878934 1103 17 3.1218853967857463 1103 18 1.8232156650275435 1103 19 3.9542972158383627 1103 20 4.624859066641509 1103 21 6.871259501182575 1103 22 2.3473664703775086 1103 23 4.715737318299645 1103 24 5.955143531228355 1103 25 2.1315052023763172 1104 1 5.814789710931976 1104 2 10.320413385891184 1104 3 7.064417270162544 1104 4 7.8400345745060385 1104 5 7.639130087745286 1104 6 7.123014389569402 1104 7 7.276889481449839 1104 8 7.219160783866567 1104 9 6.096103361381349 1104 10 5.175501033596234 1104 11 8.067633621228605 1104 12 5.057106289338769 1104 13 9.19276129621424 1104 14 7.117322185979997 1104 15 6.578190258876706 1104 16 5.998902092431514 1104 17 6.322422620960726 1104 18 4.168895872520729 1104 19 4.611374374782862 1104 20 4.103357862809041 1104 21 3.1187470073757737 1104 22 5.243164668136696 1104 23 5.664570495454729 1104 24 6.498018403897309 1104 25 5.677667160400173 1105 1 3.0042277602965513 1105 2 9.018671202855836 1105 3 5.794566125851443 1105 4 5.2032321144412705 1105 5 2.2248916465119883 1105 6 4.387579115941623 1105 7 5.3803782016174715 1105 8 2.656029099647448 1105 9 3.5952412501886 1105 10 3.2221628019038526 1105 11 6.337969158823508 1105 12 3.8769070894040816 1105 13 5.0973984094479725 1105 14 3.66897048380023 1105 15 3.991495834842091 1105 16 4.251236160518705 1105 17 4.866853458852255 1105 18 2.5221437567017517 1105 19 2.3306466963329235 1105 20 1.6441007805982473 1105 21 2.4891239088915427 1105 22 3.845501185403414 1105 23 1.3971179142916412 1105 24 4.158191479250398 1105 25 4.798962504237764 1106 1 6.4534044736668115 1106 2 10.782027344921653 1106 3 7.7330987640614985 1106 4 8.8698966088477 1106 5 8.582322948782 1106 6 7.84505603567661 1106 7 7.3553266104368475 1106 8 7.109368949761489 1106 9 6.245896186649986 1106 10 5.767744424582251 1106 11 7.687011192059092 1106 12 5.223049155223567 1106 13 4.41691616767541 1106 14 5.676028157309517 1106 15 5.960629103007616 1106 16 5.829017196686838 1106 17 6.25811011139923 1106 18 6.386127681975999 1106 19 6.30185338689445 1106 20 5.643413260233309 1106 21 5.592285633666172 1106 22 6.246732856957667 1106 23 6.774953256965691 1106 24 7.583815461003411 1106 25 6.4629746688950895 1107 1 3.7581031118724013 1107 2 -13.465773241588609 1107 3 -5.356941904535226 1107 4 1.5559387884680298 1107 5 4.3057312227809454 1107 6 4.504729708691933 1107 7 4.705428802253596 1107 8 5.28083786867197 1107 9 3.3694025939312056 1107 10 2.7171048411752032 1107 11 5.454642922722254 1107 12 2.992902673348567 1107 13 3.92942269775331 1107 14 3.575944697190341 1107 15 3.293126621057688 1107 16 3.142572805893675 1107 17 3.7393451130330497 1107 18 -0.9383667182870781 1107 19 1.790234531151416 1107 20 2.1020583549220397 1107 21 -1.4075077973356223 1107 22 1.866920631819312 1107 23 -1.2382021183030538 1107 24 2.1484335737224427 1107 25 2.899491905178806 1108 1 4.658631111392815 1108 2 9.748972642448612 1108 3 0.0343375938118875 1108 4 5.342383735640137 1108 5 6.586288820350106 1108 6 6.393864542384531 1108 7 6.62539265056893 1108 8 6.341513868596781 1108 9 5.007920801608307 1108 10 4.04009646928634 1108 11 6.162101824058535 1108 12 2.1667360880519766 1108 13 2.7245916012080933 1108 14 4.1945586489159545 1108 15 4.2143813832394486 1108 16 4.345590729833905 1108 17 -0.3215767752472729 1108 18 2.9281301296172586 1108 19 4.056442714254924 1108 20 4.327142218600315 1108 21 4.478718542895624 1108 22 1.4539049076654038 1108 23 4.083142464253196 1108 24 4.7466413321097 1108 25 5.149330223424382 1109 1 3.3769315719799557 1109 2 -13.465773241588609 1109 3 -5.442653396878285 1109 4 1.783681309992506 1109 5 1.5787961924573501 1109 6 2.563858135485951 1109 7 3.4899290060662373 1109 8 3.630405516881005 1109 9 3.415464768167219 1109 10 2.689354249911627 1109 11 0.7753938429828864 1109 12 1.1761069598284415 1109 13 2.48994781016064 1109 14 3.2990093564921636 1109 15 3.447751098852457 1109 16 3.1872031187305616 1109 17 3.877829595338593 1109 18 3.8067350535379454 1109 19 2.5005743499340447 1109 20 1.4634140297074154 1109 21 0.5188863535519452 1109 22 2.72337290552003 1109 23 3.178463341351353 1109 24 4.277731485729181 1109 25 4.627558768140151 1110 1 2.7153150115302154 1110 2 8.46980508930451 1110 3 5.627474319550267 1110 4 7.148394524288893 1110 5 7.4921255275379615 1110 6 3.6362706185716327 1110 7 6.212697490159307 1110 8 6.63844328542913 1110 9 5.13309386121837 1110 10 4.527160361689113 1110 11 2.486656323293982 1110 12 4.992374797229036 1110 13 9.386938022267234 1110 14 6.903650815669433 1110 15 6.228076280587985 1110 16 5.886014100977466 1110 17 6.402556511832028 1110 18 6.391082534425301 1110 19 5.228032955212594 1110 20 5.318384170869926 1110 21 3.3963295918805336 1110 22 5.351591961391701 1110 23 5.843880020468095 1110 24 6.730604038222253 1110 25 7.11319600567478 1111 1 4.7668732629420525 1111 2 8.90363512781976 1111 3 6.995643566928884 1111 4 7.534910806119964 1111 5 7.706921468541811 1111 6 7.038988447350956 1111 7 6.872537893777164 1111 8 7.092681336299684 1111 9 5.569151784099134 1111 10 4.822566842307893 1111 11 6.577680558534908 1111 12 5.814598287095279 1111 13 7.3971098091593745 1111 14 5.729605404435151 1111 15 4.9899662533125095 1111 16 4.59628666556921 1111 17 6.046314738313534 1111 18 5.98313461769261 1111 19 4.902700568386795 1111 20 4.484043060540529 1111 21 7.071769411537044 1111 22 6.39291821282044 1111 23 6.269681646855371 1111 24 6.2531121141710315 1111 25 6.789977415252675 1112 1 -2.251063010533361 1112 2 7.419751130641819 1112 3 5.706078790658051 1112 4 -0.13020735161267183 1112 5 4.167292215470901 1112 6 2.7254137896108617 1112 7 5.277894477124885 1112 8 5.676889102929902 1112 9 4.345187089676536 1112 10 2.9244572381421605 1112 11 -0.2838722985866333 1112 12 3.531399129450037 1112 13 7.4418428538924175 1112 14 5.026351084489544 1112 15 3.4653084066458 1112 16 3.633087465294679 1112 17 4.51618329089996 1112 18 4.510321446947387 1112 19 4.218539907121258 1112 20 3.9690769827974925 1112 21 -1.6127514672231236 1112 22 2.1358113774270464 1112 23 3.470839671499366 1112 24 5.249571751694187 1112 25 5.188963051313642 1113 1 -18.161698165629375 1113 2 -13.465773241588609 1113 3 -7.483046914872018 1113 4 -6.522559026622032 1113 5 -1.8833244700711589 1113 6 -1.371246988545444 1113 7 -20.06036253912327 1113 8 -9.465208156270991 1113 9 -5.490938117359352 1113 10 -4.645260733606776 1113 11 -5.879314407791316 1113 12 -3.9286260020077037 1113 13 -2.4003033358431978 1113 14 -2.8390336634263136 1113 15 -3.116338967300642 1113 16 -3.528255804229868 1113 17 -3.0538941939327238 1113 18 -4.017421936518687 1113 19 -3.498527890384434 1113 20 -4.484425225968195 1113 21 -1.7977904393602877 1113 22 -2.7192577570538177 1113 23 -3.1426240360568407 1113 24 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2205 19 6.123453685666686 2205 20 6.441598770628801 2205 21 0.1356404978393897 2205 22 4.655660374983732 2205 23 6.0458545814943 2205 24 7.84837318048854 2205 25 8.25155724079777 2206 1 3.8872069438596775 2206 2 8.397730408046426 2206 3 5.677004622742494 2206 4 1.7744838040871223 2206 5 5.578214166111881 2206 6 5.794251189475013 2206 7 5.750586365986219 2206 8 5.5342934954300915 2206 9 4.29679842780417 2206 10 3.474376913825992 2206 11 3.303029986074119 2206 12 4.4074991413100015 2206 13 7.549872050972709 2206 14 0.45836221129414767 2206 15 2.4080732882078664 2206 16 3.103888228645225 2206 17 3.8339797091901815 2206 18 -1.06255481047857 2206 19 0.5846883292066707 2206 20 2.09161091847335 2206 21 2.8017543495511816 2206 22 -2.4469075737206722 2206 23 -3.1502570519154425 2206 24 0.5929684459981672 2206 25 2.126921412050688 2207 1 1.177260014854852 2207 2 -13.465773241588609 2207 3 -6.113826037404991 2207 4 -4.400986792653644 2207 5 0.8303822581801477 2207 6 1.4071253239251043 2207 7 2.5038340859684425 2207 8 2.5775584059162995 2207 9 0.5100517172954717 2207 10 0.10913761237095798 2207 11 -1.6685703373858338 2207 12 -2.5377354474835023 2207 13 0.032451804736144396 2207 14 -5.429229565111484 2207 15 -1.6374387049801982 2207 16 -0.7101822791976069 2207 17 0.4685599342197726 2207 18 0.4507761308220175 2207 19 0.1084706685075929 2207 20 -0.16290011611651423 2207 21 -3.67489119939356 2207 22 -4.0567291887712 2207 23 -1.5222418857137932 2207 24 0.2189903705848728 2207 25 0.6752995602960219 2208 1 4.608496820443047 2208 2 6.479909209664873 2208 3 6.115280043111319 2208 4 8.010156699193153 2208 5 3.55756780456419 2208 6 6.020382169691754 2208 7 6.559414619651104 2208 8 6.717836589588643 2208 9 5.104414880643567 2208 10 4.567213869684725 2208 11 6.706056438303545 2208 12 5.939872115711726 2208 13 8.189753717402166 2208 14 3.351913844988715 2208 15 4.699474788726338 2208 16 5.181997534706262 2208 17 6.013191022042186 2208 18 0.9576178101423771 2208 19 3.4110500802181996 2208 20 4.473754138691717 2208 21 6.832303523046086 2208 22 6.181657174261353 2208 23 2.0984508349560365 2208 24 3.050720108157858 2208 25 4.538381672709857 2209 1 2.28445568052447 2209 2 4.191369615554249 2209 3 4.537757009042323 2209 4 0.4019215731307284 2209 5 4.489938451558793 2209 6 4.758535566898075 2209 7 5.093483614722885 2209 8 3.7371035807219286 2209 9 3.6063035212508687 2209 10 2.8583532780943273 2209 11 -0.7293568485024977 2209 12 2.7937080662050597 2209 13 1.6337842458338088 2209 14 3.055124329954076 2209 15 3.516794980141235 2209 16 3.267792937075484 2209 17 0.48166827366706855 2209 18 2.700328534886527 2209 19 3.2553923210785456 2209 20 3.266860243357712 2209 21 5.38507832484596 2209 22 4.124841844744072 2209 23 0.04680452397824908 2209 24 -3.218926957868039 2209 25 -0.32120029592503485 2210 1 -2.6034530824221136 2210 2 -13.465773241588609 2210 3 -7.634569303072187 2210 4 -2.6179938619132983 2210 5 -1.1344673493976885 2210 6 -1.806064633708175 2210 7 -2.091376154857006 2210 8 -20.032859789241442 2210 9 -12.925928694339893 2210 10 -7.60260774776836 2210 11 -10.196211375720111 2210 12 -5.07035127334596 2210 13 -5.5861450432331505 2210 14 -4.5642014778740005 2210 15 -5.9644765974994804 2210 16 -5.62409414709602 2210 17 -5.161397787855876 2210 18 -5.3522496987809625 2210 19 -10.280133827004333 2210 20 -7.3461597673930115 2210 21 -5.368232509554133 2210 22 -9.561125138348478 2210 23 -7.551244275432122 2210 24 -10.308602939979725 2210 25 -7.735398452785825 2211 1 -0.25528307128975314 2211 2 4.2836607206755435 2211 3 0.2749993893035736 2211 4 1.1171804023518241 2211 5 0.6563521636328815 2211 6 -0.29161026211567886 2211 7 -20.06036253912327 2211 8 -12.798142808109366 2211 9 -9.00035623408813 2211 10 -7.369283738976652 2211 11 -9.564043419792842 2211 12 -5.011688298436589 2211 13 -6.703817523466073 2211 14 -4.918828258338134 2211 15 -5.453325968092919 2211 16 -11.533417927531609 2211 17 -7.929149336395824 2211 18 -5.945557151753874 2211 19 -8.574105225400132 2211 20 -6.749190624013913 2211 21 -4.535502226677988 2211 22 -5.965442210823003 2211 23 -7.997123874667128 2211 24 -21.15958629852738 2211 25 -20.93585231057705 2212 1 -1.150212713868271 2212 2 -13.465773241588609 2212 3 -7.596758245993604 2212 4 -18.91602714481799 2212 5 -19.52251576089542 2212 6 -20.25538384702134 2212 7 -9.699719353824646 2212 8 -9.207438656010202 2212 9 -5.148015513466326 2212 10 -5.300787683037445 2212 11 -5.81616049916526 2212 12 -2.867353587812186 2212 13 -5.90272069894066 2212 14 -3.6861801032339727 2212 15 -3.5835152252225697 2212 16 -3.802587218756436 2212 17 -3.849929480289031 2212 18 -3.010718814905079 2212 19 -3.575388128691184 2212 20 -3.6860642695974306 2212 21 -1.6384933561529813 2212 22 -2.9162752311356357 2212 23 -2.9972250044310655 2212 24 -1.9727562832134424 2212 25 -2.410584316702625 2213 1 -18.161698165629375 2213 2 -13.465773241588609 2213 3 -8.66999683711152 2213 4 -6.259227144817988 2213 5 -19.52251576089542 2213 6 -10.863383847021339 2213 7 -20.06036253912327 2213 8 -10.573659789241443 2213 9 -8.717292083144734 2213 10 -7.5519705314294825 2213 11 -6.700964174509824 2213 12 -5.713137355040363 2213 13 -18.558157146107582 2213 14 -12.339296182866438 2213 15 -7.351348609602354 2213 16 -6.629297172814628 2213 17 -5.331398392999599 2213 18 -5.394508095150101 2213 19 -6.261501451815224 2213 20 -6.207454774957309 2213 21 -4.450490905923271 2213 22 -5.434498814596587 2213 23 -4.969667270893545 2213 24 -4.1779862985273795 2213 25 -5.04305231057705 2214 1 -18.161698165629375 2214 2 -13.465773241588609 2214 3 -12.339646837111522 2214 4 -18.91602714481799 2214 5 -19.52251576089542 2214 6 -20.25538384702134 2214 7 -16.40679111055184 2214 8 -14.850716932098585 2214 9 -14.348984940287592 2214 10 -14.199384817143766 2214 11 -14.429471317366966 2214 12 -12.873965926468934 2214 13 -18.558157146107582 2214 14 -16.652339040009295 2214 15 -22.872948609602354 2214 16 -18.098347172814627 2214 17 -22.584998392999598 2214 18 -17.579708095150103 2214 19 -15.181787166100941 2214 20 -14.062169060671593 2214 21 -11.636762334494701 2214 22 -12.576463100310873 2214 23 -12.576638699464974 2214 24 -11.54351486995595 2214 25 -11.27960231057705 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 160006.0 3 10.0 4 719.0 5 2920.0 6 4054.0 7 3921.0 8 3143.0 9 3010.0 10 2873.0 11 2691.0 12 2557.0 13 2686.0 14 3961.0 15 7897.0 16 11164.0 17 11840.0 18 12515.0 19 14363.0 20 17105.0 21 20058.0 22 21130.0 23 22957.0 24 24081.0 25 24150.0 26 20496.0 27 16257.0 28 17344.0 29 20500.0 30 19604.0 31 22199.0 32 28390.0 33 35779.0 34 42811.0 35 49033.0 36 48403.0 37 49988.0 38 34656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22554699207564 18.660917018069416 12.483322741102894 25.63021324875205 2 13.830892791708632 20.014977643664235 42.07776761024487 24.07636195438226 3 18.830277736172903 24.025265180495452 29.475144346436842 27.669312736894803 4 11.27671391516541 17.357471624312396 38.727717478005516 32.63809698251668 5 12.462064235441645 37.46390399123982 36.910799808076675 13.163231965241861 6 32.00257287320093 37.466495937725725 18.045929666839783 12.485001522233565 7 27.654009187266222 31.381707964315225 22.64606218790958 18.318220660508974 8 24.487769405678918 37.32063149283352 21.012575593350128 17.17902350813743 9 25.571757110950198 16.133749094877263 20.129674767727657 38.16481902644488 10 13.53778861729805 30.3024587855661 34.99835943171748 21.16139316541837 11 33.30409139679733 22.983622266160104 24.319483902313937 19.392802434728623 12 22.404093937874737 25.39671171493782 32.06665756782578 20.13253677936167 13 27.660213709322544 21.51068394873647 25.731003025677285 25.0980993162637 14 19.99793004857917 22.26103381136274 28.54915811194556 29.191878028112527 15 22.53728268983767 30.670662029317107 23.92048458641043 22.871570694434787 16 21.59790751455702 29.287843987055762 27.134100350627733 21.980148147759486 17 21.107103509784373 28.50558732490425 28.711898641204552 21.675410524106812 18 20.732985646613287 28.51380083625765 29.78240498394049 20.97080853318857 19 22.057677922943657 27.91680716019621 27.90311307823584 22.122401838624288 20 22.304700269695978 28.14287757437071 28.621996567505718 20.93042558842759 21 21.985860001225397 26.77782247215555 27.44259954340697 23.793717983212083 22 21.665064291269147 28.023798833091146 28.72099387819273 21.590142997446982 23 21.759410937581666 27.76425775050259 28.272267994844686 22.204063317071057 24 21.384628532919745 28.00304307804823 28.638418720253984 21.973909668778045 25 22.100291299885903 27.5925371136452 28.386377109055793 21.920794477413104 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 809.0 1 809.0 2 1667.0 3 2525.0 4 2525.0 5 2525.0 6 3698.5 7 4872.0 8 4872.0 9 4872.0 10 5867.5 11 6863.0 12 6863.0 13 6863.0 14 8653.5 15 10444.0 16 10444.0 17 10444.0 18 14594.0 19 18744.0 20 18744.0 21 18744.0 22 25589.0 23 32434.0 24 32434.0 25 32434.0 26 41610.5 27 50787.0 28 50787.0 29 50787.0 30 60562.5 31 70338.0 32 70338.0 33 70338.0 34 77812.0 35 85286.0 36 85286.0 37 85286.0 38 89828.0 39 94370.0 40 94370.0 41 94370.0 42 95865.5 43 97361.0 44 97361.0 45 97361.0 46 95579.0 47 93797.0 48 93797.0 49 93797.0 50 86950.5 51 80104.0 52 80104.0 53 80104.0 54 69257.0 55 58410.0 56 58410.0 57 58410.0 58 48118.5 59 37827.0 60 37827.0 61 37827.0 62 30120.0 63 22413.0 64 22413.0 65 22413.0 66 16615.0 67 10817.0 68 10817.0 69 10817.0 70 7721.5 71 4626.0 72 4626.0 73 4626.0 74 3144.5 75 1663.0 76 1663.0 77 1663.0 78 1115.5 79 568.0 80 568.0 81 568.0 82 369.5 83 171.0 84 171.0 85 171.0 86 100.0 87 29.0 88 29.0 89 29.0 90 20.5 91 12.0 92 12.0 93 12.0 94 6.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.237977717246658 2 36.40271957069597 3 5.625064468189962 4 13.78925237274775 5 13.477258169472705 6 14.670476816283806 7 9.5432022830335 8 7.5365065053975 9 0.10786085313223077 10 1.4197646417606151 11 5.644675532395822 12 5.762341917630984 13 16.076615588758532 14 1.5671023124500967 15 2.935801780531816 16 2.919246986072324 17 2.907403940805149 18 1.4754142200590623 19 1.4280420389903614 20 0.27595568918246055 21 4.392496348394376 22 1.5867133766559571 23 0.10671475197734286 24 9.107683844176087 25 0.10900695428711872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 785271.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.73178403917947 #Duplication Level Percentage of deduplicated Percentage of total 1 97.74816555263325 93.5760627491065 2 1.7507909773396335 3.3521268748084356 3 0.24801986378536262 0.7123015211198114 4 0.09435747357452497 0.3613203713087603 5 0.045672628239929694 0.21861610915833396 6 0.025796793955429227 0.1481743864706617 7 0.01679792251596967 0.11256665634239753 8 0.014181183139280129 0.1086071969367695 9 0.009394702104786668 0.0809434433707076 >10 0.04101879484841206 0.6884064112232006 >50 0.0035097838074855253 0.23324877690872728 >100 0.0022943240559385007 0.40762550324568037 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.273445727653256E-4 0.0 0.0 0.0 6 0.0 1.273445727653256E-4 0.0 0.0 0.0 7 0.0 2.546891455306512E-4 0.0 0.0 0.0 8 0.0 2.546891455306512E-4 0.0 0.0 0.0 9 0.0 2.546891455306512E-4 0.0 1.273445727653256E-4 0.0 10 0.0 2.546891455306512E-4 0.0 1.273445727653256E-4 0.0 11 0.0 2.546891455306512E-4 0.0 1.273445727653256E-4 0.0 12 0.0 2.546891455306512E-4 0.0 1.273445727653256E-4 0.0 13 0.0 2.546891455306512E-4 0.0 1.273445727653256E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 40 5.4238824E-4 20.019354 1 CTAGTAC 30 0.00756601 18.265923 3 TAGGACG 120 0.0 16.115652 4 GATATAC 105 5.570837E-8 15.252841 1 TATTAAG 65 6.844743E-4 14.750188 2 GTCCTAG 75 1.5183269E-4 14.235984 1 GTGTAAA 85 2.7701963E-5 14.131309 1 ACTGTTC 155 0.0 13.850645 8 CTACACC 70 3.4351484E-5 13.8134165 4 GTAGGAC 180 0.0 13.39501 3 GTCTATA 90 4.7429356E-5 13.346235 1 TCTACAC 145 1.10958354E-10 12.8491335 3 TATTAGA 140 9.770338E-8 12.718274 2 TAGGACC 110 1.0003714E-7 12.697181 4 CTATTAG 85 4.3801733E-4 12.561164 1 AGGACGT 110 1.4656143E-7 12.330776 5 TGTAGGA 235 0.0 12.239517 2 GTCCAGG 120 4.1464154E-6 12.23405 1 TATTATG 90 5.6981307E-4 12.174759 2 TATGGCG 150 0.0 12.047185 16 >>END_MODULE