FastQCFastQC Report
Thu 26 May 2016
SRR1926644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785271
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14510.18477697508248744No Hit
GGTATCAACGCAGAGTACTTTTTTT9310.11855779724451813No Hit
CACACACACACACACACACACACAC7950.10123893534843387No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTC350.002166180616.288787
CTACGGA456.544195E-414.8402744
TCTACGG400.00512594214.31209853
GTCTAGT706.960552E-613.6253271
TAGGACG1950.012.2309944
GTAGGAC3300.012.14359953
TAGGACC2000.011.925224
CCTGTAC1406.2937033E-1011.5859853
TTCAGTA1104.672911E-711.2783692
TGTAGGA3900.011.25612452
TAGCACC855.0911032E-511.2237364
GGACGTG2050.011.168276
TATACTG1552.8921932E-1011.0789145
TCCAACG600.00599815211.05153818
ACTGTTC2100.010.8564138
TGGCGAG3150.010.82599718
GACGTGA1158.772149E-710.7411527
GGCGAGA2300.010.708323519
TGAGTTA1951.8189894E-1210.69401212
GTCTAGA909.125983E-510.5974771
Produced by FastQC (version 0.11.5)