##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926644_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 785271 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.982585629674343 31.0 28.0 31.0 16.0 34.0 2 28.49149911304505 31.0 28.0 33.0 20.0 34.0 3 28.847345693397568 31.0 28.0 33.0 23.0 34.0 4 32.57436222654345 35.0 32.0 37.0 26.0 37.0 5 32.186442642094256 35.0 32.0 37.0 23.0 37.0 6 32.24558528202366 35.0 32.0 37.0 23.0 37.0 7 32.05146121530019 35.0 32.0 37.0 19.0 37.0 8 32.000236860905346 35.0 32.0 37.0 19.0 37.0 9 33.188403748514844 37.0 33.0 39.0 17.0 39.0 10 33.09759306023016 37.0 33.0 39.0 17.0 39.0 11 33.31022920749652 37.0 33.0 39.0 19.0 39.0 12 33.110170883682194 37.0 33.0 39.0 17.0 39.0 13 33.24142620827714 37.0 33.0 39.0 18.0 39.0 14 33.87895261635792 38.0 33.0 40.0 17.0 41.0 15 33.979775134953414 38.0 33.0 40.0 17.0 41.0 16 33.94386778577077 38.0 33.0 40.0 17.0 41.0 17 33.84780540730525 38.0 33.0 40.0 17.0 41.0 18 33.802326840033565 38.0 33.0 40.0 17.0 41.0 19 33.84830205113904 39.0 33.0 40.0 17.0 41.0 20 33.820160174003625 39.0 33.0 40.0 15.0 41.0 21 33.744069244884884 39.0 33.0 40.0 11.0 41.0 22 33.6635874748972 39.0 33.0 40.0 10.0 41.0 23 33.312473018868644 38.0 33.0 40.0 2.0 41.0 24 33.381966225672414 39.0 33.0 40.0 2.0 41.0 25 33.18280568109608 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6935348428829613 1101 2 0.9419777549986748 1101 3 0.8256019757871798 1101 4 0.6794713703127186 1101 5 0.6930366949355928 1101 6 0.21393424836095676 1101 7 0.7142173427441243 1101 8 0.7440890910270319 1101 9 1.1728883396397336 1101 10 0.9295953468898759 1101 11 0.7201090988507843 1101 12 0.8139379918550489 1101 13 0.8588329659446288 1101 14 1.1706675301562939 1101 15 1.0090883267326944 1101 16 0.9947202913008724 1101 17 0.9452009395360221 1101 18 1.131833936738019 1101 19 0.9584969719367677 1101 20 0.833997099373974 1101 21 1.074269532040283 1101 22 0.9426133824168517 1101 23 1.264532813304747 1101 24 1.2038788586988147 1101 25 1.3071002216231165 1102 1 0.6426708447947291 1102 2 0.6813180119949216 1102 3 0.7740183622579764 1102 4 0.634358348517182 1102 5 0.8578810747173691 1102 6 0.4609753637594878 1102 7 0.6375363973067643 1102 8 0.6975979326732507 1102 9 0.7640537760397308 1102 10 0.9502960501233915 1102 11 0.8752135295949657 1102 12 0.939812302989516 1102 13 0.664113461879154 1102 14 1.2523019817320176 1102 15 0.8322029586069561 1102 16 0.892061282510312 1102 17 0.8156079845225293 1102 18 0.8455426919871343 1102 19 0.9589057091819768 1102 20 0.8802233354391973 1102 21 0.9329270032002484 1102 22 0.7729117335729683 1102 23 1.202918917535989 1102 24 0.9864711935778914 1102 25 1.1934312587830647 1103 1 0.42403344065672144 1103 2 0.39721953213593153 1103 3 0.31399664051921405 1103 4 -3.2486162296407883E-4 1103 5 0.2338118218548999 1103 6 0.15035776262477896 1103 7 0.2770974811312463 1103 8 0.3977514641417663 1103 9 0.6796946788243474 1103 10 0.8247140527350041 1103 11 0.4696364704486413 1103 12 0.5147079771873635 1103 13 0.517643461971609 1103 14 0.6129932486032104 1103 15 0.592333289039523 1103 16 0.596404705701886 1103 17 0.4296598937132643 1103 18 0.4905086491168831 1103 19 0.5586263830671072 1103 20 0.5951698387232938 1103 21 0.5550013803046383 1103 22 0.44509730270264924 1103 23 0.7778015391116497 1103 24 0.6461936839529372 1103 25 0.6622547987520946 1104 1 0.32084865522823236 1104 2 0.39476400758639585 1104 3 0.3236142476363746 1104 4 0.10900934043489485 1104 5 0.10181935539334575 1104 6 -0.1290319837790861 1104 7 0.25105586828398785 1104 8 0.2089866174638466 1104 9 0.6119597275459725 1104 10 0.668150568859609 1104 11 0.3035750284928369 1104 12 0.5070422809469051 1104 13 0.2614683479923059 1104 14 0.5197523858466369 1104 15 0.4376401371521439 1104 16 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0.8071272487987997 2202 20 0.8731827535055103 2202 21 1.0096213876739597 2202 22 1.1419922986321325 2202 23 1.3986849658657476 2202 24 1.1867439480201298 2202 25 1.1837116844439919 2203 1 0.08886104376430382 2203 2 0.08521978165152078 2203 3 0.013020689254954476 2203 4 -0.08954116546166091 2203 5 0.08634142537574263 2203 6 0.06544337487643759 2203 7 0.25706271603513997 2203 8 0.2799182842493799 2203 9 0.5727059588226098 2203 10 0.4001710756241579 2203 11 0.20810236172333418 2203 12 0.2279906165276202 2203 13 0.07418185646132258 2203 14 0.4719712706240742 2203 15 0.6028541584008522 2203 16 0.3549788800587024 2203 17 0.4091890332910566 2203 18 0.4799421094915246 2203 19 0.42851625646579095 2203 20 0.6009582662125581 2203 21 0.4787147737779165 2203 22 0.50503562556802 2203 23 0.6466604898429011 2203 24 0.6180078906353899 2203 25 0.6039789848884993 2204 1 -0.009591809239630322 2204 2 0.16859448845155356 2204 3 0.07559226535234487 2204 4 -0.300731750405248 2204 5 -0.4316825116571472 2204 6 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1.0654439674711398 2205 18 1.117443620371489 2205 19 1.2011868062300906 2205 20 1.258389236692956 2205 21 1.2445136781040915 2205 22 1.1784416335890242 2205 23 1.6083462072087116 2205 24 1.401233366195072 2205 25 1.3873536428307176 2206 1 0.17474472778115313 2206 2 0.5250664365646607 2206 3 0.7295223122303547 2206 4 0.5793301114437597 2206 5 0.8937247276775722 2206 6 0.5765081675694788 2206 7 0.9364836076555285 2206 8 0.9616003568246647 2206 9 1.1179021164077483 2206 10 1.0208034593383815 2206 11 0.9219588917964359 2206 12 0.8655673028018072 2206 13 0.6748381866146147 2206 14 1.0112329953169166 2206 15 0.9480843682617319 2206 16 0.8878195538543778 2206 17 1.1002607462853007 2206 18 1.0157513728089853 2206 19 1.1162047110966284 2206 20 1.1128485519770592 2206 21 1.1534523790241593 2206 22 1.1175756507227632 2206 23 1.2997803922996098 2206 24 1.433145225660354 2206 25 0.9882177242952181 2207 1 -0.7998267145610392 2207 2 0.20865727043798543 2207 3 0.2442295649093431 2207 4 0.5336183656704279 2207 5 0.4888594401784374 2207 6 0.4439019050849424 2207 7 0.6608603849447867 2207 8 0.7040678635589508 2207 9 0.9690382427070787 2207 10 0.7060790697083803 2207 11 0.4509926355240452 2207 12 0.5913626983172833 2207 13 0.37458755870601834 2207 14 -4.665569463835553 2207 15 -1.5129087180495944 2207 16 -0.6483008546476938 2207 17 -0.4075151452378023 2207 18 0.21159566218782544 2207 19 0.17061185292096326 2207 20 0.406871504780824 2207 21 0.34959281633773287 2207 22 0.6565327601475559 2207 23 0.6000331623270796 2207 24 0.540061056454384 2207 25 0.4894869297297717 2208 1 0.13350893492589933 2208 2 0.7217970142032755 2208 3 0.46320366554349235 2208 4 0.28855586017807866 2208 5 0.5513920110885024 2208 6 0.4995079845794237 2208 7 0.5760094600842187 2208 8 0.41604803183432537 2208 9 0.8669566677771243 2208 10 0.7316049557904094 2208 11 0.5026575071716692 2208 12 0.5535794208100597 2208 13 0.565302371393912 2208 14 0.8963101561245139 2208 15 0.5842777684203213 2208 16 0.6136770655962422 2208 17 0.4243259720746977 2208 18 0.7110597110559382 2208 19 0.34597414362412593 2208 20 0.5054354214238188 2208 21 0.7590630575106303 2208 22 0.6020062466603875 2208 23 1.1113463805475803 2208 24 1.0567092620143796 2208 25 1.0584350758191192 2209 1 -0.32307885982345397 2209 2 -0.26408358616200545 2209 3 0.30060864682033994 2209 4 0.5284116868027056 2209 5 0.5875858543854093 2209 6 0.4173546689999199 2209 7 0.6167167565018197 2209 8 0.7097862342076233 2209 9 0.7997956486662474 2209 10 0.4083652892359808 2209 11 0.5862566378460059 2209 12 0.43147997893346 2209 13 0.6082544054322909 2209 14 0.6302378347597113 2209 15 0.47119604976757046 2209 16 0.4165834906475112 2209 17 0.5700155656751562 2209 18 0.6447078178238215 2209 19 0.6784009090592491 2209 20 0.5627456135529556 2209 21 0.697638363356134 2209 22 0.5859160694281513 2209 23 0.9674122738001074 2209 24 0.7140066999524848 2209 25 0.7370559906517329 2210 1 -0.5343931632708916 2210 2 -0.5338003545172008 2210 3 0.28422377098009477 2210 4 0.4535850006295732 2210 5 0.4604086946817745 2210 6 0.6136129744866707 2210 7 0.5789761813936174 2210 8 0.4850706572983974 2210 9 0.7476546479624631 2210 10 0.8068928836788558 2210 11 0.4358451733649531 2210 12 0.5478061137164829 2210 13 0.18277020733084726 2210 14 0.6086909499753119 2210 15 0.45780863032871366 2210 16 0.7759607246087086 2210 17 0.6429741753962475 2210 18 0.5935465736217296 2210 19 0.6557046945324743 2210 20 0.8022971865387163 2210 21 0.9027815308291025 2210 22 1.0082964122320917 2210 23 1.3143402827553459 2210 24 0.9727887650885592 2210 25 0.9939489212209125 2211 1 -1.371491327858564 2211 2 -0.6422603779237015 2211 3 0.032764289919381184 2211 4 0.26194683090388793 2211 5 0.564731476059336 2211 6 0.35446988083211295 2211 7 0.4366075575246917 2211 8 0.43893130297533034 2211 9 0.79115648908369 2211 10 0.8292540859738295 2211 11 0.45169308818812226 2211 12 0.35404822829416105 2211 13 0.4099462984449431 2211 14 0.6855533368676703 2211 15 0.45029343037438707 2211 16 0.5111138659326073 2211 17 0.6036101471942601 2211 18 0.5000758460220425 2211 19 0.5030577616406262 2211 20 0.5588752700599713 2211 21 0.542376769603969 2211 22 0.5285658724553102 2211 23 0.757786176626837 2211 24 0.566264630700978 2211 25 0.584804971230902 2212 1 1.1188689830432246 2212 2 0.3241252443132865 2212 3 0.7244843627328095 2212 4 0.6592330859242566 2212 5 0.7769878255638005 2212 6 0.7044331851749135 2212 7 0.7985307566925393 2212 8 0.848816015044445 2212 9 0.9425910870206451 2212 10 0.7461329325166375 2212 11 0.5649988757000415 2212 12 0.6582367341741389 2212 13 0.6431752273362719 2212 14 0.5674753802667851 2212 15 0.6990853631086438 2212 16 0.07936894409146333 2212 17 0.5665365536203382 2212 18 0.7325408117823926 2212 19 0.555172529475584 2212 20 0.6653764691700275 2212 21 0.5246755936092882 2212 22 0.6306260302177407 2212 23 0.7497530060642674 2212 24 0.8146243059299678 2212 25 0.23025615444913683 2213 1 0.2277841438395498 2213 2 -0.03617735905577746 2213 3 -0.5754470308353383 2213 4 -1.4967399615489434 2213 5 -2.081562863563306 2213 6 -0.7793867229414708 2213 7 -0.6855660273809647 2213 8 -0.6075441687227823 2213 9 -1.3470321901615918 2213 10 -1.109176102705412 2213 11 -1.0297257797364097 2213 12 -1.2717932811398 2213 13 -1.135789550611662 2213 14 -7.797888172566296 2213 15 -3.77500468283316 2213 16 -2.67833519067117 2213 17 -1.9868049471453588 2213 18 -5.54375811624211 2213 19 -3.556957332698996 2213 20 -6.01944925824192 2213 21 -2.7997213436035757 2213 22 -2.5137586558465763 2213 23 -4.792523257335425 2213 24 -2.710837256091473 2213 25 -2.05822899103325 2214 1 0.12165557241097602 2214 2 -0.11108450191292008 2214 3 -1.8234756022639083 2214 4 -2.2110113901203725 2214 5 -0.4839342921347338 2214 6 -0.7451224372271845 2214 7 -0.6385945988095365 2214 8 -0.9315370258656372 2214 9 -5.464032190161593 2214 10 -1.8451832455625556 2214 11 -1.1625757797364074 2214 12 -1.2196647097112283 2214 13 -1.6486824077545208 2214 14 -2.8878024582805786 2214 15 -2.382833254261733 2214 16 -1.9816994763854545 2214 17 -2.1874478042882153 2214 18 -1.4500724019563975 2214 19 -4.899071618413281 2214 20 -2.6010135439562028 2214 21 -4.264678486460717 2214 22 -6.91971579870372 2214 23 -6.9012232573354275 2214 24 -6.146501541805758 2214 25 -3.906236133890392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31618.0 3 20.0 4 623.0 5 3768.0 6 4689.0 7 3724.0 8 2884.0 9 2304.0 10 1965.0 11 1852.0 12 1752.0 13 1685.0 14 1651.0 15 1679.0 16 1739.0 17 1900.0 18 2043.0 19 2416.0 20 3099.0 21 4083.0 22 5314.0 23 6506.0 24 7699.0 25 8929.0 26 10217.0 27 11297.0 28 13025.0 29 15405.0 30 18692.0 31 23761.0 32 29974.0 33 38310.0 34 49258.0 35 64568.0 36 85542.0 37 124723.0 38 196557.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.082029726567576 19.410209809104558 9.13272445954592 30.375036004781947 2 13.532444901879362 21.37942103345757 39.06928695350893 26.018847111154138 3 18.154442692238124 24.354110990240013 28.926605200740852 28.564841116781015 4 11.802995523783562 17.65583322081224 37.166608495384935 33.37456276001926 5 13.137829837316772 38.37793153344101 34.952947710053955 13.531290919188265 6 31.775260041115644 38.35043928282859 17.263968580282903 12.610332095772861 7 28.381297763985142 32.04763223342448 20.93365710865912 18.637412893931256 8 25.293451045550952 38.01668185286994 19.310980263040353 17.378886838538758 9 25.691190133439513 16.29669617241978 19.617241977804323 38.39487171633639 10 14.0090607005804 30.64883225986405 33.90133592477351 21.440771114782034 11 34.495080572655475 23.59669610087946 23.011153079131084 18.897070247333982 12 22.11730096976103 26.04588376876007 31.33811740284879 20.49869785863011 13 28.2153549538949 22.330252868118137 25.695333203441866 23.759058974545095 14 20.380605507603573 22.580180630230522 27.563760279445443 29.475453582720462 15 22.914820135089702 30.613455993968845 23.290875841763707 23.180848029177746 16 22.081744146805814 29.589496405626196 26.52679702771711 21.801962419850877 17 21.49872268676408 29.13713224712891 27.309160742802856 22.054984323304154 18 21.23780318524386 29.13456420026336 28.326155115721697 21.301477498771085 19 22.58149550848256 28.083890899551484 27.565462561349264 21.76915103061669 20 22.521655192789158 28.203707807075855 28.075852390667066 21.19878460946792 21 22.526675572319803 27.463964577743422 27.60735493187685 22.402004918059927 22 22.052259665771434 28.298128926192355 27.570609381984053 22.07900202605215 23 22.137580529524204 27.910492046694706 27.424035778731167 22.527891645049923 24 21.859643001315472 27.87758080352083 28.309280004686293 21.953496190477402 25 22.27625876926564 27.73768546145216 27.74723630440956 22.238819464872638 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 308.0 1 308.0 2 305.5 3 303.0 4 303.0 5 303.0 6 441.5 7 580.0 8 580.0 9 580.0 10 1022.5 11 1465.0 12 1465.0 13 1465.0 14 2525.0 15 3585.0 16 3585.0 17 3585.0 18 6317.5 19 9050.0 20 9050.0 21 9050.0 22 14167.5 23 19285.0 24 19285.0 25 19285.0 26 26699.0 27 34113.0 28 34113.0 29 34113.0 30 44432.0 31 54751.0 32 54751.0 33 54751.0 34 64045.0 35 73339.0 36 73339.0 37 73339.0 38 81230.0 39 89121.0 40 89121.0 41 89121.0 42 95406.0 43 101691.0 44 101691.0 45 101691.0 46 104546.0 47 107401.0 48 107401.0 49 107401.0 50 103490.0 51 99579.0 52 99579.0 53 99579.0 54 87822.5 55 76066.0 56 76066.0 57 76066.0 58 64651.5 59 53237.0 60 53237.0 61 53237.0 62 42929.0 63 32621.0 64 32621.0 65 32621.0 66 24903.5 67 17186.0 68 17186.0 69 17186.0 70 12211.0 71 7236.0 72 7236.0 73 7236.0 74 5095.0 75 2954.0 76 2954.0 77 2954.0 78 1964.0 79 974.0 80 974.0 81 974.0 82 644.5 83 315.0 84 315.0 85 315.0 86 202.0 87 89.0 88 89.0 89 89.0 90 52.5 91 16.0 92 16.0 93 16.0 94 11.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08315600601575761 2 0.027633772290075655 3 0.029161907163259566 4 0.029671285454320864 5 0.02381343510711589 6 0.3931126961265601 7 0.203751316424521 8 0.2120287136542671 9 0.2158490508372269 10 0.015536037877369723 11 0.002164857737010535 12 0.0033109588918984654 13 0.0 14 0.0730957847672969 15 0.0019101685914798842 16 0.00203751316424521 17 0.0036929926101944427 18 0.0031836143191331405 19 0.0016554794459492327 20 0.0011461011548879305 21 2.546891455306512E-4 22 0.0 23 0.0 24 2.546891455306512E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 785271.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.5459417648906 #Duplication Level Percentage of deduplicated Percentage of total 1 93.73501020474654 82.99854754920914 2 4.9353663959792415 8.74013330973552 3 0.683124695828835 1.814637586050559 4 0.23296613849751904 0.8251282453037103 5 0.12482124992380053 0.5526207563386848 6 0.0713345180239617 0.3789829247265751 7 0.043628762746578134 0.2704204919802915 8 0.028693810870966653 0.20325764051147185 9 0.02364334331491894 0.18841698902611148 >10 0.10639259455949174 1.6555532073686912 >50 0.007509733374614715 0.46430447274657055 >100 0.006497785534119972 1.1505772021520633 >500 8.663713709458442E-4 0.5719004020859094 >1k 1.4439522849097404E-4 0.18551922276473387 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1451 0.18477697508248744 No Hit GGTATCAACGCAGAGTACTTTTTTT 931 0.11855779724451813 No Hit CACACACACACACACACACACACAC 795 0.10123893534843387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.273445727653256E-4 0.0 12 0.0 0.0 0.0 1.273445727653256E-4 0.0 13 0.0 0.0 0.0 1.273445727653256E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCGTC 35 0.0021661806 16.28878 7 CTACGGA 45 6.544195E-4 14.840274 4 TCTACGG 40 0.005125942 14.3120985 3 GTCTAGT 70 6.960552E-6 13.625327 1 TAGGACG 195 0.0 12.230994 4 GTAGGAC 330 0.0 12.1435995 3 TAGGACC 200 0.0 11.92522 4 CCTGTAC 140 6.2937033E-10 11.585985 3 TTCAGTA 110 4.672911E-7 11.278369 2 TGTAGGA 390 0.0 11.2561245 2 TAGCACC 85 5.0911032E-5 11.223736 4 GGACGTG 205 0.0 11.16827 6 TATACTG 155 2.8921932E-10 11.078914 5 TCCAACG 60 0.005998152 11.051538 18 ACTGTTC 210 0.0 10.856413 8 TGGCGAG 315 0.0 10.825997 18 GACGTGA 115 8.772149E-7 10.741152 7 GGCGAGA 230 0.0 10.7083235 19 TGAGTTA 195 1.8189894E-12 10.694012 12 GTCTAGA 90 9.125983E-5 10.597477 1 >>END_MODULE