FastQCFastQC Report
Thu 26 May 2016
SRR1926643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274714
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC57322.08653363134023No Hit
GTACATGGGAAGCAGTGGTATCAAC56212.0461279730920157No Hit
GCTTCCCATGTACTCTGCGTTGATA35901.3068136316314423No Hit
GAGTACATGGGAAGCAGTGGTATCA23620.8598032863268709No Hit
CATGTACTCTGCGTTGATACCACTG20500.7462306253048625No Hit
GTATCAACGCAGAGTACATGGGAAG16360.5955284404871977No Hit
GCGTTGATACCACTGCTTCCCATGT14860.540926199611232No Hit
CNCATGTACTCTGCGTTGATACCAC13700.49870046666715195No Hit
ACGCAGAGTACATGGGAAGCAGTGG12710.46266298768901476No Hit
GNACATGGGAAGCAGTGGTATCAAC12380.45065049469630236No Hit
GCTTACCATGTACTCTGCGTTGATA10950.39859635839454854No Hit
GCTTTCCATGTACTCTGCGTTGATA9880.3596467599030264No Hit
TATCAACGCAGAGTACATGGGAAGC9450.34399411751858294No Hit
GTACATGGTAAGCAGTGGTATCAAC8990.32724943031662024No Hit
GAGTAAGCAGTGGTATCAACGCAGA8750.3185130717764657No Hit
CAGTGGTATCAACGCAGAGTACATG8570.31196080287134986No Hit
GGTATCAACGCAGAGTACATGGGAA8550.3112327729930036No Hit
ACTCTGCGTTGATACCACTGCTTCC8430.3068645937229264No Hit
GNTTCCCATGTACTCTGCGTTGATA8270.3010403546961567No Hit
GCAGTGGTATCAACGCAGAGTACAT7770.28283960773750155No Hit
CTGCTTCCCATGTACTCTGCGTTGA7490.27264718944065464No Hit
GTACTCTGCGTTGATACCACTGCTT7200.2620907562046347No Hit
CCATGTACTCTGCGTTGATACCACT7170.26099871138711533No Hit
GCTTACTTCCCATGTACTCTGCGTT7130.2595426516304229No Hit
CTTCCCATGTACTCTGCGTTGATAC6940.25262636778613395No Hit
ACCATGTACTCTGCGTTGATACCAC6850.24935023333357598No Hit
ATGGTAAGCAGTGGTATCAACGCAG6760.24607409888101808No Hit
CATGGGAAGCAGTGGTATCAACGCA6600.2402498598542484No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA6490.2362456955233443No Hit
GTGGTATCAACGCAGAGTACATGGG6380.23224153119244012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6280.22860138180070907No Hit
AAGCAGTGGTATCAACGCAGAGTAC6170.22459721746980496No Hit
GCTTACTCTGCGTTGATACCACTGC5790.210764649781227No Hit
CCACTGCTTCCCATGTACTCTGCGT5700.20748851532866908No Hit
GNGTACATGGGAAGCAGTGGTATCA5660.20603245557197666No Hit
GGGAAGCAGTGGTATCAACGCAGAG5650.2056684406328036No Hit
GTACATGGAAAGCAGTGGTATCAAC5050.18382754428241735No Hit
ATGGAAAGCAGTGGTATCAACGCAG4780.17399914092474356No Hit
TACCACTGCTTCCCATGTACTCTGC4450.16198664793203113No Hit
ATACCACTGCTTCCCATGTACTCTG4430.1612586180536849No Hit
CNTGTACTCTGCGTTGATACCACTG4390.1598025582969925No Hit
ATGGGAAGCAGTGGTATCAACGCAG4270.15543437902691526No Hit
GCAGAGTACATGGGAAGCAGTGGTA4080.1485180951826263No Hit
TCCATGTACTCTGCGTTGATACCAC3800.13832567688577938No Hit
GATACCACTGCTTCCCATGTACTCT3780.13759764700743318No Hit
ACATGGGAAGCAGTGGTATCAACGC3730.13577757231156765No Hit
CATGGAAAGCAGTGGTATCAACGCA3720.13541355737239455No Hit
GAGTACATGGTAAGCAGTGGTATCA3670.13359348267652907No Hit
GNGTTGATACCACTGCTTCCCATGT3560.1295893183456249No Hit
CTGCTTACTCTGCGTTGATACCACT3490.12704121377141317No Hit
CCCNTGTACTCTGCGTTGATACCAC3460.12594916895389388No Hit
GTANATGGGAAGCAGTGGTATCAAC3420.12449310919720145No Hit
GTACATGGGAAGTAAGCAGTGGTAT3420.12449310919720145No Hit
GTTGATACCACTGCTTCCCATGTAC3390.12340106437968214No Hit
GNATCAACGCAGAGTACATGGGAAG3380.12303704944050904No Hit
GAGTACATGGAAAGCAGTGGTATCA3290.1197609149879511No Hit
CCCATGTACTCTGCTTTGATACCAC3280.119396900048778No Hit
ACTCTGCGTTGATACCACTGCTTAC3220.11721281041373938No Hit
GGTAAGCAGTGGTATCAACGCAGAG3210.11684879547456627No Hit
TCCCATGTACTCTGCGTTGATACCA3210.11684879547456627No Hit
GTGGTAAGCAGTGGTATCAACGCAG3190.11612076559622006No Hit
GTACATGGGAAGCATTGGTATCAAC3170.11539273571787385No Hit
GCTTGCTTCCCATGTACTCTGCGTT3120.11357266102200833No Hit
NNCNNNNACTCNNCGTTGATACCNC3020.10993251163027731No Hit
GGAAGCAGTGGTATCAACGCAGAGT2990.108840466812758No Hit
GCGTTGATACCACTGCTTACCATGT2900.10556433236020006No Hit
CTGCTTACCATGTACTCTGCGTTGA2820.10265221284681524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCAT200.00742005527.8201562
CGCAGAC250.003884667320.4913777
GTAAAAA404.8084036E-420.3675061
GCAGACT250.005067912419.5717358
GGAAGAA250.005067912419.5717358
GAACAAA350.00532983919.3976251
TTAGCAT357.834051E-418.9468083
CCCATGG501.7512884E-618.8859485
TAGCATG359.0593996E-418.5418784
CCATGGG300.00718961218.420513
GAAGAAG309.607503E-418.3825119
GGAGTCT250.007280274318.38251110
AGCATGT350.001111277417.9866185
TGTACTA350.001111277417.9866185
CATGTAG709.6988515E-917.5640377
TACAGGG805.430229E-717.3875982
GTCCATG552.0388766E-417.2815191
AAAAAGT552.0388766E-417.2815191
GGAAGGA853.45608E-1117.2691788
ACAGGGA452.124272E-417.1924743
Produced by FastQC (version 0.11.5)