##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926643_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 274714 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.982119586187817 27.0 10.0 31.0 2.0 33.0 2 16.75968097730731 20.0 2.0 31.0 2.0 33.0 3 20.724466172091702 25.0 15.0 31.0 2.0 33.0 4 22.83118443180908 28.0 7.0 35.0 2.0 37.0 5 23.540889798117316 30.0 7.0 35.0 2.0 37.0 6 24.41180282038775 31.0 8.0 35.0 2.0 37.0 7 24.310330015943855 32.0 10.0 35.0 2.0 37.0 8 24.503134168626282 32.0 17.0 35.0 2.0 37.0 9 27.168622640273156 32.0 17.0 38.0 2.0 39.0 10 28.001627146778105 33.0 26.0 39.0 2.0 39.0 11 24.804844310810516 30.0 10.0 37.0 2.0 39.0 12 26.194860837088754 31.0 17.0 37.0 2.0 39.0 13 22.518182546211698 29.0 2.0 37.0 2.0 39.0 14 26.16528826343033 31.0 17.0 38.0 2.0 40.0 15 27.172681406844937 31.0 19.0 38.0 2.0 40.0 16 27.432908406561005 32.0 23.0 39.0 2.0 40.0 17 26.893307221328364 31.0 17.0 38.0 2.0 40.0 18 27.018411875623375 31.0 18.0 38.0 2.0 40.0 19 27.3793363279629 31.0 23.0 39.0 2.0 40.0 20 27.711434437269304 32.0 26.0 39.0 2.0 40.0 21 25.557019300072074 31.0 10.0 38.0 2.0 40.0 22 26.541865358154297 31.0 17.0 38.0 2.0 40.0 23 26.5508456067037 31.0 16.0 39.0 2.0 40.0 24 25.52241968010367 31.0 2.0 39.0 2.0 40.0 25 25.281627437990053 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.827919435769033 1101 2 10.918108841472513 1101 3 7.497350513072952 1101 4 8.617345171464049 1101 5 8.481949852932992 1101 6 7.682366270524607 1101 7 7.474368712698428 1101 8 5.743856657264736 1101 9 5.440168205426783 1101 10 5.095625138794581 1101 11 7.928231254649752 1101 12 5.965885787583634 1101 13 10.264987132968532 1101 14 7.529286681364113 1101 15 6.482585953305541 1101 16 6.3372225035690875 1101 17 1.7668894495800735 1101 18 4.6551325095662754 1101 19 5.158989647624445 1101 20 3.4028519323204307 1101 21 0.3439655671552053 1101 22 4.316258049912612 1101 23 5.537115529158143 1101 24 7.134070497417923 1101 25 7.7240632042142074 1102 1 6.06322263269751 1102 2 11.03927185202604 1102 3 7.472071240051015 1102 4 8.14345406382576 1102 5 8.191341915598194 1102 6 7.409006698067969 1102 7 7.588344904552724 1102 8 7.4482999441008 1102 9 6.018417708619847 1102 10 5.250503493447205 1102 11 8.489748493367891 1102 12 5.485159967128695 1102 13 9.960565068952786 1102 14 7.708021415403586 1102 15 5.685220477046162 1102 16 6.0417767278750105 1102 17 6.70445094752532 1102 18 6.703096083489214 1102 19 4.956977338388661 1102 20 5.505811551081408 1102 21 4.450996108722993 1102 22 5.27749263925104 1102 23 6.067883795253721 1102 24 7.499836158894645 1102 25 7.2077216325678926 1103 1 6.005780414514966 1103 2 10.570215338711751 1103 3 7.44317533721636 1103 4 8.965049581115359 1103 5 8.05630126356759 1103 6 7.651430637819065 1103 7 7.71754245144739 1103 8 7.112626039308456 1103 9 6.451551610615493 1103 10 5.436578924438635 1103 11 6.385136935006759 1103 12 5.070719007461104 1103 13 9.489346107327503 1103 14 7.626177990644798 1103 15 6.68207918377222 1103 16 6.413937578071774 1103 17 4.408255458202717 1103 18 3.153979041598646 1103 19 5.258736262857788 1103 20 5.938495983451212 1103 21 7.8885348130518835 1103 22 3.7405262595790525 1103 23 6.24906999553739 1103 24 7.617127754811463 1103 25 3.7652287741408372 1104 1 6.706607655761598 1104 2 11.692839424898747 1104 3 8.121820970152108 1104 4 9.029478207510127 1104 5 8.609783372070861 1104 6 8.373689198804062 1104 7 8.265580158257698 1104 8 8.264165092790567 1104 9 6.93923918275863 1104 10 6.144658955219704 1104 11 9.349450132390366 1104 12 5.665216891262567 1104 13 10.352389585588373 1104 14 8.094281107228099 1104 15 7.202336975230477 1104 16 6.562956431476934 1104 17 7.390691010338156 1104 18 4.490435370956092 1104 19 5.756908636847783 1104 20 4.986668357441204 1104 21 4.086514805356618 1104 22 6.082311561618301 1104 23 6.2684235930749 1104 24 7.6192247151808346 1104 25 6.845374984221639 1105 1 4.31627782067914 1105 2 10.321531328946723 1105 3 7.105385437918223 1105 4 5.773143874676542 1105 5 3.2953156059539204 1105 6 5.500263411697613 1105 7 6.537900248212722 1105 8 4.158018166253832 1105 9 4.416000801949032 1105 10 4.157646211591519 1105 11 7.279266474219451 1105 12 4.870726636390003 1105 13 6.594949555603364 1105 14 4.968831332115659 1105 15 5.200350468483851 1105 16 5.135538890247545 1105 17 5.874592809438607 1105 18 3.5109938417207083 1105 19 3.097613284523945 1105 20 2.574786798220817 1105 21 4.162470577470561 1105 22 4.938439747525344 1105 23 2.2692294401513635 1105 24 5.2980478036366065 1105 25 6.046049646890303 1106 1 6.824180119529714 1106 2 11.796100294463965 1106 3 8.61529923102167 1106 4 9.528028932147809 1106 5 9.12318916917231 1106 6 8.314631227789565 1106 7 8.60471059304031 1106 8 8.059454947863028 1106 9 7.074837008845581 1106 10 6.50118069435014 1106 11 8.70814578456428 1106 12 6.3501806593785055 1106 13 5.26226277399417 1106 14 6.8983571941846265 1106 15 6.682318859288447 1106 16 6.736688315534906 1106 17 6.9941330393236605 1106 18 6.800217979651741 1106 19 6.901564433949233 1106 20 6.216740821209321 1106 21 6.574467703907342 1106 22 7.247347793502353 1106 23 7.71815185394447 1106 24 8.4252029760504 1106 25 7.061135853786855 1107 1 3.9526083895719104 1107 2 -14.628899705536035 1107 3 -6.041029882902379 1107 4 2.2841470756077236 1107 5 4.604096342168091 1107 6 4.793639666608133 1107 7 4.789309749158452 1107 8 5.576649040690032 1107 9 3.8416882746683676 1107 10 3.2545562217762978 1107 11 6.240846206505207 1107 12 3.1857819252012938 1107 13 5.041033871040582 1107 14 4.029448966336524 1107 15 3.76726084241081 1107 16 3.289483674184691 1107 17 4.210008566749824 1107 18 -0.34524615536935244 1107 19 2.1794916491391056 1107 20 2.538576264247297 1107 21 -1.6057801863880172 1107 22 2.2528857681859016 1107 23 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0.19619152261001815 1109 12 0.1311664528642993 1109 13 1.4566018245832204 1109 14 2.4100905621679907 1109 15 2.5956028163224047 1109 16 2.3437482600948485 1109 17 2.944817377508002 1109 18 3.1584820129157762 1109 19 1.3275043161391125 1109 20 0.10497713444563317 1109 21 -0.16134743815780084 1109 22 2.3354888191433822 1109 23 2.562005804048418 1109 24 3.733285096632521 1109 25 4.0690620492130485 1110 1 3.111401179501822 1110 2 9.379468495300785 1110 3 6.017600486251798 1110 4 7.280051247350038 1110 5 7.64198275355167 1110 6 3.325126346339079 1110 7 5.789857036554952 1110 8 6.611163455533877 1110 9 4.782289937715877 1110 10 4.165825045814572 1110 11 2.4183968305893835 1110 12 4.864179962028437 1110 13 9.576166191009513 1110 14 6.749220164895924 1110 15 5.8170904771406065 1110 16 5.533776878993756 1110 17 6.321416860802042 1110 18 6.259109193040864 1110 19 4.717978311215617 1110 20 5.1697045590056945 1110 21 2.980020353837606 1110 22 5.293808741898449 1110 23 5.36713197946748 1110 24 6.493456114125713 1110 25 6.833886899811958 1111 1 5.62462777793192 1111 2 9.662422608513552 1111 3 7.764886007881174 1111 4 8.581885681459102 1111 5 8.652183659530433 1111 6 7.674066489497555 1111 7 8.212148609569233 1111 8 8.316824093868536 1111 9 6.392347339424095 1111 10 5.826800529060883 1111 11 8.592443305225437 1111 12 7.32872749133443 1111 13 9.018271727162212 1111 14 7.031776615672229 1111 15 6.2177699612168205 1111 16 6.0176456158103875 1111 17 7.242307970453137 1111 18 6.879350211056703 1111 19 6.01005272596025 1111 20 5.381961206884249 1111 21 8.453169494540951 1111 22 7.556404267331555 1111 23 7.268926647332897 1111 24 7.503198843818993 1111 25 8.233139986018262 1112 1 -2.6668670630907876 1112 2 7.352770355563759 1112 3 5.396052795176455 1112 4 -1.4996017535276849 1112 5 3.776125353829471 1112 6 2.9407174463910586 1112 7 5.60827474782646 1112 8 5.6440951379512825 1112 9 4.233912874426032 1112 10 2.996394543637308 1112 11 -1.2078419954764534 1112 12 3.2120866335808174 1112 13 7.977635313030149 1112 14 5.4372192104779025 1112 15 3.4810671620718807 1112 16 3.6225420154670154 1112 17 4.829307852629839 1112 18 4.410350362543802 1112 19 4.448708867078899 1112 20 4.5276539237212035 1112 21 -1.3913121671042 1112 22 2.559678071845873 1112 23 3.897034236836525 1112 24 5.557713159349788 1112 25 5.525367014682985 1113 1 -19.821653213803618 1113 2 -14.628899705536035 1113 3 -7.726925391008567 1113 4 -6.009840038334552 1113 5 -1.1395358128291306 1113 6 -0.4572010256301624 1113 7 -22.14528940695969 1113 8 -10.117452899509184 1113 9 -4.907060939318562 1113 10 -4.332620601546189 1113 11 -5.498657671159261 1113 12 -3.1792379871297705 1113 13 -1.6796603721541388 1113 14 -2.510808572554037 1113 15 -2.8315535309203383 1113 16 -3.16812629929591 1113 17 -2.6316678966014635 1113 18 -3.1361211132208275 1113 19 -3.0680045005360093 1113 20 -3.666862490525741 1113 21 -1.058879128346078 1113 22 -2.2315488947625823 1113 23 -2.681834647135446 1113 24 -2.39593633280489 1113 25 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6.169531200586338 2204 9 4.183129492505714 2204 10 3.863598995003734 2204 11 5.532219858638353 2204 12 5.749445365260861 2204 13 4.146753514734911 2204 14 5.159754253008153 2204 15 5.353165809179512 2204 16 5.392652367822485 2204 17 0.10990101317987211 2204 18 3.7809914001310467 2204 19 4.582569335910016 2204 20 4.6152702329740265 2204 21 6.035183934844163 2204 22 5.4471026954631405 2204 23 4.152206864837712 2204 24 6.3164066797541025 2204 25 6.161217553133259 2205 1 6.317175419602023 2205 2 11.338562333509518 2205 3 7.877446736444664 2205 4 7.997515555430528 2205 5 8.27577412940369 2205 6 7.788944315280528 2205 7 8.121521872866772 2205 8 7.946240920386526 2205 9 6.112892865678557 2205 10 5.220595011052957 2205 11 8.07636704269877 2205 12 6.960963378105912 2205 13 10.00181899236004 2205 14 7.436901120431912 2205 15 6.566262279389672 2205 16 5.793259175983202 2205 17 6.761369120307034 2205 18 6.74777763258016 2205 19 5.106196574223993 2205 20 5.662312043190525 2205 21 -1.2398173647839066 2205 22 3.888300432692521 2205 23 4.993680595809977 2205 24 6.859991479304199 2205 25 7.222876634697915 2206 1 4.518009233453768 2206 2 9.134813374632742 2206 3 6.66572117194994 2206 4 1.4254972865781887 2206 5 6.002830519383281 2206 6 6.456086923992096 2206 7 6.877917344095163 2206 8 6.943737648284969 2206 9 5.115949878043896 2206 10 4.670168036122291 2206 11 4.196542409036855 2206 12 5.318693317606357 2206 13 8.849050748677715 2206 14 -0.20261854421200098 2206 15 2.657556201060597 2206 16 3.865433041273299 2206 17 5.009586625821552 2206 18 -1.1406048051583824 2206 19 1.3714747715019726 2206 20 3.230559386610164 2206 21 3.6283970288018565 2206 22 -1.5445825862444806 2206 23 -2.5020221966884435 2206 24 1.988230402210739 2206 25 3.6605556850104826 2207 1 1.127174078307256 2207 2 -14.628899705536035 2207 3 -6.950479020577475 2207 4 -4.83963275939448 2207 5 1.3735267668979674 2207 6 1.6996880863538877 2207 7 2.6308299960253834 2207 8 2.801334834145898 2207 9 0.25797933293939934 2207 10 -0.285919518869477 2207 11 -2.518400057653203 2207 12 -3.4114309255539723 2207 13 0.11674482800980712 2207 14 -5.8747371554955485 2207 15 -2.0344943958643604 2207 16 -1.0734485003854957 2207 17 0.028097147354934293 2207 18 0.02074036771144705 2207 19 -0.4601582028666904 2207 20 -0.37741340764640086 2207 21 -4.329161471643474 2207 22 -5.154260237769854 2207 23 -1.2900171226013768 2207 24 0.3568394366047727 2207 25 0.9781134657271515 2208 1 4.127012083937654 2208 2 6.2951249351210485 2208 3 5.678851592417974 2208 4 7.360404017705644 2208 5 2.227005048706875 2208 6 5.155373869815577 2208 7 5.464566855873986 2208 8 5.590238657650843 2208 9 3.4165849554574947 2208 10 3.284650920222827 2208 11 5.4335051274800925 2208 12 4.7189263814866536 2208 13 7.46008532704003 2208 14 2.2125384056835955 2208 15 3.6187194410817405 2208 16 3.8208173367532474 2208 17 4.753792519132013 2208 18 -0.5318662092599595 2208 19 2.115403414099813 2208 20 2.9204198082045316 2208 21 5.372230366467232 2208 22 4.4809634676981105 2208 23 0.19711745969395267 2208 24 2.004900101307072 2208 25 3.06254755809897 2209 1 2.135313999311137 2209 2 4.305526523972162 2209 3 4.5984959523331455 2209 4 -1.1971076798740512 2209 5 4.176604469718757 2209 6 4.818592976423446 2209 7 5.326022068450147 2209 8 3.7339084997755947 2209 9 3.1001443858947653 2209 10 2.122492169759976 2209 11 -0.7795591334685064 2209 12 2.393691588340257 2209 13 1.2639362712619295 2209 14 2.540058013856754 2209 15 2.728049733605385 2209 16 2.3882593537862675 2209 17 0.03559478795754245 2209 18 2.0104638812910878 2209 19 2.2233198984301055 2209 20 1.9297189632858256 2209 21 4.18426961647565 2209 22 3.2128532579832303 2209 23 -0.9106391296620906 2209 24 -4.413936368211896 2209 25 -0.657101851131177 2210 1 -1.4642064052929804 2210 2 -14.628899705536035 2210 3 -7.8049135349357766 2210 4 -1.2946661033131868 2210 5 0.1400331408035136 2210 6 -0.4873191268203634 2210 7 -0.4346511090873513 2210 8 -22.298878385470307 2210 9 -13.595774693282076 2210 10 -6.470095901394544 2210 11 -10.212066981393166 2210 12 -4.597780333529293 2210 13 -4.888313467140584 2210 14 -3.674914082411121 2210 15 -5.51745950950588 2210 16 -4.718542180918998 2210 17 -4.645849230179884 2210 18 -5.2386118075822985 2210 19 -10.742628828462117 2210 20 -7.573507405741033 2210 21 -5.856870948574926 2210 22 -11.395116745504737 2210 23 -9.349800273715108 2210 24 -12.078786385651728 2210 25 -9.554023720681231 2211 1 0.022228642736465076 2211 2 4.995572868303626 2211 3 0.7479996529625978 2211 4 1.8896323076752353 2211 5 1.3129571016617625 2211 6 -0.02281602959440221 2211 7 -22.14528940695969 2211 8 -14.071030284204484 2211 9 -9.082362358243024 2211 10 -7.0640091790675825 2211 11 -10.398394299823906 2211 12 -4.913374192942168 2211 13 -7.446307901111316 2211 14 -4.816542594844911 2211 15 -5.14202185801113 2211 16 -12.23794248615286 2211 17 -7.053704513418953 2211 18 -5.290393834694246 2211 19 -8.109537886725875 2211 20 -6.282098841237936 2211 21 -4.221813941662276 2211 22 -5.209139548269796 2211 23 -7.337465234663128 2211 24 -23.3872970239496 2211 25 -23.126364146213145 2212 1 -0.9648202420031851 2212 2 -14.628899705536035 2212 3 -8.19413677765512 2212 4 -20.705304401185526 2212 5 -21.368477497494357 2212 6 -22.193702105543768 2212 7 -10.600820860321948 2212 8 -9.448553005860761 2212 9 -5.015089780742269 2212 10 -5.408472234066348 2212 11 -5.617776124329428 2212 12 -2.785240526449403 2212 13 -6.453712461002215 2212 14 -3.855940528158108 2212 15 -3.782978501521388 2212 16 -3.783747331608989 2212 17 -4.156201378608369 2212 18 -3.438773343558669 2212 19 -3.8504194778367413 2212 20 -3.94289403056219 2212 21 -1.8059778853912825 2212 22 -3.2310054241404487 2212 23 -3.214562354298483 2212 24 -2.066255809199056 2212 25 -2.631787139705555 2213 1 -19.821653213803618 2213 2 -14.628899705536035 2213 3 -9.012770944416925 2213 4 -5.5583745766241215 2213 5 -21.368477497494357 2213 6 -11.45466701782447 2213 7 -22.14528940695969 2213 8 -11.329580139856272 2213 9 -7.9910621139614335 2213 10 -7.482591235474423 2213 11 -6.5615910575409835 2213 12 -5.287319340621494 2213 13 -20.260653892672497 2213 14 -12.920612104060991 2213 15 -7.039062719658922 2213 16 -6.249934491482641 2213 17 -4.871437136114935 2213 18 -4.848466230874571 2213 19 -5.765211881840244 2213 20 -5.70825917879068 2213 21 -3.7868261557417817 2213 22 -4.551994311760801 2213 23 -4.524063058336235 2213 24 -3.70966544500223 2213 25 -5.126364146213145 2214 1 -19.821653213803618 2214 2 -14.628899705536035 2214 3 -12.931335384362944 2214 4 -20.705304401185526 2214 5 -21.368477497494357 2214 6 -22.193702105543768 2214 7 -17.515481714651997 2214 8 -15.950320693162615 2214 9 -14.256412991154416 2214 10 -14.3872808441114 2214 11 -15.125988830820335 2214 12 -12.692768058570211 2214 13 -20.260653892672497 2214 14 -17.686258190430763 2214 15 -25.02809780737822 2214 16 -18.942959120362534 2214 17 -24.72670029400967 2214 18 -18.756993558809796 2214 19 -15.382409925025128 2214 20 -14.195438665970165 2214 21 -11.756461783272144 2214 22 -12.912107334702773 2214 23 -12.84002122297861 2214 24 -11.728643177795753 2214 25 -11.337902607751607 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 44004.0 3 0.0 4 194.0 5 770.0 6 1131.0 7 1085.0 8 983.0 9 850.0 10 775.0 11 699.0 12 703.0 13 800.0 14 1173.0 15 2690.0 16 4343.0 17 4523.0 18 4534.0 19 5177.0 20 6026.0 21 6969.0 22 7483.0 23 8063.0 24 8373.0 25 8663.0 26 7099.0 27 5372.0 28 5551.0 29 6824.0 30 6483.0 31 7485.0 32 10132.0 33 13131.0 34 16319.0 35 19070.0 36 19640.0 37 20886.0 38 16711.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.04267549207456 14.30956619268335 8.419541084454565 22.228217230787532 2 8.232384649563855 22.801150581137016 32.89445990505367 36.07200486424546 3 25.977122915858857 31.6874757658007 27.678945327646375 14.656455990694067 4 14.762256041139873 20.339676867775044 32.23732181462325 32.66074527646183 5 10.163952563115142 42.77489606692566 32.43818269243044 14.622968677528755 6 28.517453798767967 24.596995569004648 21.113152491083973 25.772398141143416 7 21.416636661178025 34.35311537885807 22.09590698163664 22.134340978327273 8 29.63059590868663 32.77517541259018 21.444806799090816 16.149421879632374 9 28.182176023792515 18.15272695464552 21.369163034128846 32.295933987433116 10 23.25719842775319 32.86927647804882 24.641603898817117 19.231921195380878 11 22.865496622486255 29.115254263577917 20.47486835064099 27.54438076329484 12 30.57435188278343 35.476503080353325 23.043606615961902 10.905538420901344 13 29.2343928854451 18.71841155234657 20.36321211587567 31.683983446332658 14 24.987039533126953 29.28287885296165 26.220875979440994 19.509205634470398 15 37.79839552491297 16.703758561836953 21.79574896806839 23.70209694518169 16 12.454872288576672 28.04762477812209 42.11769307139204 17.379809861909205 17 32.49591311373757 25.732914936394895 26.052349724722372 15.718822225145153 18 27.816177014726563 21.60105083993192 27.567157551986977 23.015614593354545 19 26.219038673461085 33.55434919273125 25.78674496520202 14.439867168605641 20 20.925502625409877 28.81487756607342 28.50121594087533 21.758403867641366 21 17.67205309754802 28.3718051102951 33.11374934564226 20.842392446514612 22 25.838530941199128 26.21446243648054 13.340938384272361 34.60606823804797 23 14.133465518309684 35.791269230909414 23.659166870168356 26.416098380612546 24 20.480515557345687 41.81129795589568 20.58483536401168 17.123351122746953 25 19.939494450620547 28.735397568843286 17.624245958703142 33.700862021833025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1979.0 1 1979.0 2 2488.5 3 2998.0 4 2998.0 5 2998.0 6 3212.0 7 3426.0 8 3426.0 9 3426.0 10 3221.5 11 3017.0 12 3017.0 13 3017.0 14 2545.0 15 2073.0 16 2073.0 17 2073.0 18 2042.0 19 2011.0 20 2011.0 21 2011.0 22 2738.0 23 3465.0 24 3465.0 25 3465.0 26 5145.5 27 6826.0 28 6826.0 29 6826.0 30 8331.0 31 9836.0 32 9836.0 33 9836.0 34 12318.5 35 14801.0 36 14801.0 37 14801.0 38 19440.5 39 24080.0 40 24080.0 41 24080.0 42 36490.0 43 48900.0 44 48900.0 45 48900.0 46 65666.0 47 82432.0 48 82432.0 49 82432.0 50 65932.5 51 49433.0 52 49433.0 53 49433.0 54 32046.0 55 14659.0 56 14659.0 57 14659.0 58 8830.0 59 3001.0 60 3001.0 61 3001.0 62 1974.0 63 947.0 64 947.0 65 947.0 66 702.5 67 458.0 68 458.0 69 458.0 70 337.5 71 217.0 72 217.0 73 217.0 74 155.0 75 93.0 76 93.0 77 93.0 78 68.5 79 44.0 80 44.0 81 44.0 82 29.5 83 15.0 84 15.0 85 15.0 86 8.0 87 1.0 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.317799602495688 2 37.73742874407566 3 6.120547187256565 4 14.632308509941247 5 14.453941189746427 6 15.794244195781795 7 10.023879380009754 8 7.9133207626841005 9 0.12449310919720145 10 1.555435835086672 11 6.127099456161681 12 6.288722089154539 13 17.317646716221233 14 1.6970376464250092 15 3.1698420903193867 16 3.2040594946016583 17 3.1374447607329805 18 1.6213225390770036 19 1.5638081786876534 20 0.3101407281754843 21 4.73474231382456 22 1.7174224830187033 23 0.1277692436497594 24 9.624190976797689 25 0.13068136316314422 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 274714.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.86648603699513 #Duplication Level Percentage of deduplicated Percentage of total 1 82.90071982294897 31.391589496325427 2 7.356469954772073 5.571273336479019 3 2.807831251644623 3.1896810865391902 4 1.5547757767163317 2.3549558095874894 5 0.9418606451201985 1.7832476483619613 6 0.6848985505090636 1.5560820839765794 7 0.4999259455784207 1.32513071864438 8 0.3759443109513093 1.1388552001060406 9 0.3069545304796914 1.0460960498161467 >10 2.1456821576839475 15.739145453086328 >50 0.23696489812259727 6.182681322289559 >100 0.15097763551270965 11.2113630934255 >500 0.025996149161128815 7.116709174223934 >1k 0.00899866701731382 6.094844086112512 >5k 0.0019997037816252934 4.298345441025925 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 5732 2.08653363134023 No Hit GTACATGGGAAGCAGTGGTATCAAC 5621 2.0461279730920157 No Hit GCTTCCCATGTACTCTGCGTTGATA 3590 1.3068136316314423 No Hit GAGTACATGGGAAGCAGTGGTATCA 2362 0.8598032863268709 No Hit CATGTACTCTGCGTTGATACCACTG 2050 0.7462306253048625 No Hit GTATCAACGCAGAGTACATGGGAAG 1636 0.5955284404871977 No Hit GCGTTGATACCACTGCTTCCCATGT 1486 0.540926199611232 No Hit CNCATGTACTCTGCGTTGATACCAC 1370 0.49870046666715195 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1271 0.46266298768901476 No Hit GNACATGGGAAGCAGTGGTATCAAC 1238 0.45065049469630236 No Hit GCTTACCATGTACTCTGCGTTGATA 1095 0.39859635839454854 No Hit GCTTTCCATGTACTCTGCGTTGATA 988 0.3596467599030264 No Hit TATCAACGCAGAGTACATGGGAAGC 945 0.34399411751858294 No Hit GTACATGGTAAGCAGTGGTATCAAC 899 0.32724943031662024 No Hit GAGTAAGCAGTGGTATCAACGCAGA 875 0.3185130717764657 No Hit CAGTGGTATCAACGCAGAGTACATG 857 0.31196080287134986 No Hit GGTATCAACGCAGAGTACATGGGAA 855 0.3112327729930036 No Hit ACTCTGCGTTGATACCACTGCTTCC 843 0.3068645937229264 No Hit GNTTCCCATGTACTCTGCGTTGATA 827 0.3010403546961567 No Hit GCAGTGGTATCAACGCAGAGTACAT 777 0.28283960773750155 No Hit CTGCTTCCCATGTACTCTGCGTTGA 749 0.27264718944065464 No Hit GTACTCTGCGTTGATACCACTGCTT 720 0.2620907562046347 No Hit CCATGTACTCTGCGTTGATACCACT 717 0.26099871138711533 No Hit GCTTACTTCCCATGTACTCTGCGTT 713 0.2595426516304229 No Hit CTTCCCATGTACTCTGCGTTGATAC 694 0.25262636778613395 No Hit ACCATGTACTCTGCGTTGATACCAC 685 0.24935023333357598 No Hit ATGGTAAGCAGTGGTATCAACGCAG 676 0.24607409888101808 No Hit CATGGGAAGCAGTGGTATCAACGCA 660 0.2402498598542484 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 649 0.2362456955233443 No Hit GTGGTATCAACGCAGAGTACATGGG 638 0.23224153119244012 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 628 0.22860138180070907 No Hit AAGCAGTGGTATCAACGCAGAGTAC 617 0.22459721746980496 No Hit GCTTACTCTGCGTTGATACCACTGC 579 0.210764649781227 No Hit CCACTGCTTCCCATGTACTCTGCGT 570 0.20748851532866908 No Hit GNGTACATGGGAAGCAGTGGTATCA 566 0.20603245557197666 No Hit GGGAAGCAGTGGTATCAACGCAGAG 565 0.2056684406328036 No Hit GTACATGGAAAGCAGTGGTATCAAC 505 0.18382754428241735 No Hit ATGGAAAGCAGTGGTATCAACGCAG 478 0.17399914092474356 No Hit TACCACTGCTTCCCATGTACTCTGC 445 0.16198664793203113 No Hit ATACCACTGCTTCCCATGTACTCTG 443 0.1612586180536849 No Hit CNTGTACTCTGCGTTGATACCACTG 439 0.1598025582969925 No Hit ATGGGAAGCAGTGGTATCAACGCAG 427 0.15543437902691526 No Hit GCAGAGTACATGGGAAGCAGTGGTA 408 0.1485180951826263 No Hit TCCATGTACTCTGCGTTGATACCAC 380 0.13832567688577938 No Hit GATACCACTGCTTCCCATGTACTCT 378 0.13759764700743318 No Hit ACATGGGAAGCAGTGGTATCAACGC 373 0.13577757231156765 No Hit CATGGAAAGCAGTGGTATCAACGCA 372 0.13541355737239455 No Hit GAGTACATGGTAAGCAGTGGTATCA 367 0.13359348267652907 No Hit GNGTTGATACCACTGCTTCCCATGT 356 0.1295893183456249 No Hit CTGCTTACTCTGCGTTGATACCACT 349 0.12704121377141317 No Hit CCCNTGTACTCTGCGTTGATACCAC 346 0.12594916895389388 No Hit GTANATGGGAAGCAGTGGTATCAAC 342 0.12449310919720145 No Hit GTACATGGGAAGTAAGCAGTGGTAT 342 0.12449310919720145 No Hit GTTGATACCACTGCTTCCCATGTAC 339 0.12340106437968214 No Hit GNATCAACGCAGAGTACATGGGAAG 338 0.12303704944050904 No Hit GAGTACATGGAAAGCAGTGGTATCA 329 0.1197609149879511 No Hit CCCATGTACTCTGCTTTGATACCAC 328 0.119396900048778 No Hit ACTCTGCGTTGATACCACTGCTTAC 322 0.11721281041373938 No Hit GGTAAGCAGTGGTATCAACGCAGAG 321 0.11684879547456627 No Hit TCCCATGTACTCTGCGTTGATACCA 321 0.11684879547456627 No Hit GTGGTAAGCAGTGGTATCAACGCAG 319 0.11612076559622006 No Hit GTACATGGGAAGCATTGGTATCAAC 317 0.11539273571787385 No Hit GCTTGCTTCCCATGTACTCTGCGTT 312 0.11357266102200833 No Hit NNCNNNNACTCNNCGTTGATACCNC 302 0.10993251163027731 No Hit GGAAGCAGTGGTATCAACGCAGAGT 299 0.108840466812758 No Hit GCGTTGATACCACTGCTTACCATGT 290 0.10556433236020006 No Hit CTGCTTACCATGTACTCTGCGTTGA 282 0.10265221284681524 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTTCAT 20 0.007420055 27.820156 2 CGCAGAC 25 0.0038846673 20.491377 7 GTAAAAA 40 4.8084036E-4 20.367506 1 GCAGACT 25 0.0050679124 19.571735 8 GGAAGAA 25 0.0050679124 19.571735 8 GAACAAA 35 0.005329839 19.397625 1 TTAGCAT 35 7.834051E-4 18.946808 3 CCCATGG 50 1.7512884E-6 18.885948 5 TAGCATG 35 9.0593996E-4 18.541878 4 CCATGGG 30 0.007189612 18.42051 3 GAAGAAG 30 9.607503E-4 18.382511 9 GGAGTCT 25 0.0072802743 18.382511 10 AGCATGT 35 0.0011112774 17.986618 5 TGTACTA 35 0.0011112774 17.986618 5 CATGTAG 70 9.6988515E-9 17.564037 7 TACAGGG 80 5.430229E-7 17.387598 2 GTCCATG 55 2.0388766E-4 17.281519 1 AAAAAGT 55 2.0388766E-4 17.281519 1 GGAAGGA 85 3.45608E-11 17.269178 8 ACAGGGA 45 2.124272E-4 17.192474 3 >>END_MODULE