FastQCFastQC Report
Thu 26 May 2016
SRR1926643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274714
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC169846.182429726915992No Hit
GTACATGGGAAGCAGTGGTATCAAC132824.834846422097163No Hit
GCTTCCCATGTACTCTGCGTTGATA84823.0875747140662653No Hit
CATGTACTCTGCGTTGATACCACTG57252.083985526766018No Hit
GAGTACATGGGAAGCAGTGGTATCA53611.9514840889070086No Hit
GCGTTGATACCACTGCTTCCCATGT38201.390537067641256No Hit
GTATCAACGCAGAGTACATGGGAAG35401.2886128846727871No Hit
ACGCAGAGTACATGGGAAGCAGTGG30441.1080614748429276No Hit
GCTTACCATGTACTCTGCGTTGATA27470.9999490379085157No Hit
ACTCTGCGTTGATACCACTGCTTCC22630.8237658073487336No Hit
TATCAACGCAGAGTACATGGGAAGC22260.8102972545993287No Hit
GCTTTCCATGTACTCTGCGTTGATA21730.7910044628231543No Hit
GTACATGGTAAGCAGTGGTATCAAC20820.7578791033584018No Hit
GAGTAAGCAGTGGTATCAACGCAGA20820.7578791033584018No Hit
GGTATCAACGCAGAGTACATGGGAA20430.7436825207306508No Hit
CTGCTTCCCATGTACTCTGCGTTGA20030.7291219231637267No Hit
GCAGTGGTATCAACGCAGAGTACAT19990.7276658634070342No Hit
CCATGTACTCTGCGTTGATACCACT19810.7211135945019184No Hit
ACCATGTACTCTGCGTTGATACCAC19800.7207495795627452No Hit
CAGTGGTATCAACGCAGAGTACATG19570.7123772359617639No Hit
CTTCCCATGTACTCTGCGTTGATAC17590.6403022780054893No Hit
ATGGTAAGCAGTGGTATCAACGCAG17030.6199174414117955No Hit
GCTTACTTCCCATGTACTCTGCGTT16830.6126371426283335No Hit
GTACTCTGCGTTGATACCACTGCTT16520.6013526795139673No Hit
CATGGGAAGCAGTGGTATCAACGCA16000.5824239026769659No Hit
AAGCAGTGGTATCAACGCAGAGTAC15220.5540307374214638No Hit
CCACTGCTTCCCATGTACTCTGCGT14800.5387421099761934No Hit
GTGGTATCAACGCAGAGTACATGGG14550.5296417364968659No Hit
GCTTACTCTGCGTTGATACCACTGC14320.5212693928958845No Hit
GGGAAGCAGTGGTATCAACGCAGAG14180.516173183747461No Hit
ATACCACTGCTTCCCATGTACTCTG13270.4830478242827086No Hit
GTACATGGAAAGCAGTGGTATCAAC13110.4772235852559389No Hit
ATGGAAAGCAGTGGTATCAACGCAG12490.4546546590272065No Hit
ATGGGAAGCAGTGGTATCAACGCAG11170.4066046870563568No Hit
TACCACTGCTTCCCATGTACTCTGC10960.3989603733337216No Hit
GCAGAGTACATGGGAAGCAGTGGTA10520.3829437160101051No Hit
GATACCACTGCTTCCCATGTACTCT9940.36183084953806505No Hit
CATGGAAAGCAGTGGTATCAACGCA9930.36146683459889195No Hit
ACATGGGAAGCAGTGGTATCAACGC8910.32433731080323536No Hit
TCCATGTACTCTGCGTTGATACCAC8750.3185130717764657No Hit
GGTAAGCAGTGGTATCAACGCAGAG8530.3105047431146574No Hit
CTGCTTACTCTGCGTTGATACCACT8500.3094126982971381No Hit
GTACATGGGAAGTAAGCAGTGGTAT8450.3075926236012726No Hit
ACTCTGCGTTGATACCACTGCTTAC8280.30140436963532985No Hit
GAGTACATGGTAAGCAGTGGTATCA8270.3010403546961567No Hit
CCCACGTACTCTGCGTTGATACCAC8190.2981282351827719No Hit
GTTGATACCACTGCTTCCCATGTAC7870.2864797571292326No Hit
GTGGTAAGCAGTGGTATCAACGCAG7790.28356763761584775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7490.27264718944065464No Hit
GAGTACATGGAAAGCAGTGGTATCA7350.2675509802922312No Hit
GCTTGCTTCCCATGTACTCTGCGTT7240.26354681596132706No Hit
TCCCATGTACTCTGCGTTGATACCA7000.2548104574211726No Hit
CCCAAGTACTCTGCGTTGATACCAC6990.25444644248199944No Hit
GCGTTGATACCACTGCTTACCATGT6920.25189833790778776No Hit
CATGGTAAGCAGTGGTATCAACGCA6830.24862220345522978No Hit
GGAAGCAGTGGTATCAACGCAGAGT6650.24206993455011394No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA6490.2362456955233443No Hit
ATCAACGCAGAGTACATGGGAAGCA6450.23478963576665188No Hit
CTGCTTACCATGTACTCTGCGTTGA6440.23442562082747875No Hit
GTACATGGGAAGCAAGCAGTGGTAT6020.21913699338220843No Hit
AAAAAGTACTCTGCGTTGATACCAC5850.21294873941626563No Hit
GCGTTGATACCACTGCTTTCCATGT5410.1969320820926491No Hit
GTAAGCAGTGGTATCAACGCAGAGT5320.19365594764009117No Hit
ATGGCAAGCAGTGGTATCAACGCAG4880.17763929031647457No Hit
GAAGTAAGCAGTGGTATCAACGCAG4830.17581921562060907No Hit
GGAAAGCAGTGGTATCAACGCAGAG4720.17181505128970492No Hit
GTACTTGGGAAGCAGTGGTATCAAC4700.17108702141135873No Hit
GTACATGGCAAGCAGTGGTATCAAC4620.1681749018979739No Hit
GCTTGCCATGTACTCTGCGTTGATA4580.1667188421412815No Hit
GCCATGTACTCTGCGTTGATACCAC4500.16380672262789664No Hit
GTACATGGGAAAGCAGTGGTATCAA4220.15361430433104975No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4080.1485180951826263No Hit
GTACGTGGGAAGCAGTGGTATCAAC4050.14742605036510698No Hit
TGGGAAGCAGTGGTATCAACGCAGA3970.14451393085172215No Hit
GTACACGGGAAGCAGTGGTATCAAC3940.14342188603420283No Hit
GTATCAACGCAGAGTACATGGTAAG3890.14160181133833732No Hit
GCTTACCACTGCTTCCCATGTACTC3860.140509766520818No Hit
GTATCAACGCAGAGTACTTTTTTTT3840.13978173664247182No Hit
AAAGTACTCTGCGTTGATACCACTG3790.13796166194660628No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3770.1372336320682601No Hit
ACTCTGCGTTGATACCACTGCTTTC3740.13614158725074077No Hit
ACGCAGAGTACATGGAAAGCAGTGG3680.13395749761570214No Hit
ACGCAGAGTACATGGTAAGCAGTGG3550.1292253034064518No Hit
GTATCAACGCAGAGTACATGGAAAG3380.12303704944050904No Hit
CTGCTTACTTCCCATGTACTCTGCG3280.119396900048778No Hit
GCTTTCCCATGTACTCTGCGTTGAT3250.11830485523125868No Hit
CCCGTGTACTCTGCGTTGATACCAC3200.11648478053539317No Hit
CTGCGTTGATACCACTGCTTCCCAT3110.11320864608283525No Hit
GCGTTGATACCACTGCTTACTTCCC3090.11248061620448904No Hit
CTCTGCGTTGATACCACTGCTTCCC3050.11102455644779663No Hit
TGGTAAGCAGTGGTATCAACGCAGA3010.10956849669110422No Hit
GAGTACATGGGAAGCAAGCAGTGGT2970.10811243693441179No Hit
GCGTTGATACCACTGCTTACTCTGC2950.10738440705606558No Hit
CATGGCAAGCAGTGGTATCAACGCA2940.10702039211689249No Hit
GAGTACATGGGAAGTAAGCAGTGGT2930.10665637717771938No Hit
ACTGCTTCCCATGTACTCTGCGTTG2900.10556433236020006No Hit
TTCCCATGTACTCTGCGTTGATACC2860.10410827260350765No Hit
GCTTACCACTGCTTGCTTCCCATGT2780.10119615309012282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATTT250.005866286319.085062
TTAGCAT401.2363989E-519.074573
TTTCCAC250.005884955619.0745683
CTTAGCA453.3892837E-516.9644992
TACATAA402.6585042E-416.6994292
TAGCATG402.6771636E-416.6841354
AGCATGT402.6771636E-416.6841355
CTTAGTA456.512707E-414.8439352
ATGTAAG456.512707E-414.8439352
TTAGTAC456.539934E-414.8357763
GTACATT456.549032E-414.8330581
GTACAAG456.549032E-414.8330581
GTAACAA400.005110616314.3137952
GCTTAGC602.4646597E-514.3033071
GGTAACA400.00513554814.3033071
TAGTACT400.005141796514.3006884
CACGGGA1006.148184E-1014.3006864
CGGGAAG1101.72804E-1013.8673336
ACACGGG1051.2714736E-913.6246933
GTACACG1400.013.6221981
Produced by FastQC (version 0.11.5)