FastQCFastQC Report
Thu 26 May 2016
SRR1926642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854526
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA4250.017.131112
ACGTGCA350.001792863416.7557588
CTGTAGG4600.015.4360761
CATAAGG550.003772795214.9860482
GTCTTAC658.0939964E-414.4279161
TCCTACA4850.014.162072
GTAGGAC4750.014.1053583
TAGGACC2100.013.8330484
GTTTAGC600.007933089513.3973491
GTCCTAG1101.4982979E-613.3973491
GTCCTAC4300.013.3973491
GTATAGT700.001416863613.3973491
GTGTTAC802.5417583E-413.3973481
TAGGACG3950.013.0742724
GGACCTG2850.012.8398456
TCCTAGC750.001972291612.8213952
TGCTATA750.001972291612.8213952
AGGACGT3700.012.717975
GTTTTAC854.242333E-412.609271
CTAGGAC1056.008886E-712.5527773
Produced by FastQC (version 0.11.5)