##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926642_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 854526 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.0459260455504 27.0 2.0 31.0 2.0 31.0 2 16.244115451138995 16.0 2.0 30.0 2.0 31.0 3 19.739116188389822 23.0 15.0 31.0 2.0 31.0 4 21.853626454900144 28.0 2.0 35.0 2.0 37.0 5 22.542181279446147 30.0 2.0 35.0 2.0 37.0 6 23.290960134624342 30.0 2.0 35.0 2.0 37.0 7 23.051277550361252 30.0 2.0 35.0 2.0 37.0 8 23.061315863999457 30.0 7.0 35.0 2.0 37.0 9 25.846569911272447 31.0 17.0 37.0 2.0 39.0 10 26.595734945455142 31.0 22.0 37.0 2.0 39.0 11 23.743680122079375 30.0 7.0 37.0 2.0 39.0 12 24.878035308463406 31.0 10.0 37.0 2.0 39.0 13 21.582851779817116 27.0 2.0 35.0 2.0 39.0 14 24.89566379489916 30.0 12.0 38.0 2.0 40.0 15 25.959585782059293 31.0 16.0 38.0 2.0 40.0 16 26.333559189538995 31.0 13.0 39.0 2.0 40.0 17 25.682985655205343 31.0 10.0 38.0 2.0 40.0 18 25.775570316175283 31.0 10.0 38.0 2.0 40.0 19 26.167236573258158 31.0 11.0 39.0 2.0 40.0 20 26.3976777769196 31.0 10.0 39.0 2.0 40.0 21 24.311734224587667 30.0 2.0 38.0 2.0 40.0 22 25.221907817901386 31.0 2.0 38.0 2.0 40.0 23 25.2140344471672 31.0 2.0 38.0 2.0 40.0 24 24.253904503783385 31.0 2.0 39.0 2.0 40.0 25 24.065176483805057 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.111165254176882 1101 2 10.688157266388584 1101 3 7.217861826776872 1101 4 8.761705409168112 1101 5 8.4550807385302 1101 6 7.914812929441712 1101 7 7.79172213781839 1101 8 5.970239682474983 1101 9 6.17832977685163 1101 10 5.627757221867572 1101 11 8.247903713772345 1101 12 5.919951322270556 1101 13 10.3337642621186 1101 14 7.717699108689967 1101 15 7.017190653594245 1101 16 6.624612339331733 1101 17 2.9703730487890105 1101 18 5.363158996134153 1101 19 5.609243149250496 1101 20 3.732141396990425 1101 21 0.6760945151512203 1101 22 4.400885332343943 1101 23 5.609447366328542 1101 24 7.056473767041645 1101 25 7.684913752184624 1102 1 6.171034702168935 1102 2 10.977738648537015 1102 3 7.317108787658572 1102 4 8.34232884230751 1102 5 8.305117160648361 1102 6 7.356401312205065 1102 7 7.431801604258013 1102 8 7.402846465503224 1102 9 6.47276996416538 1102 10 5.980668626247688 1102 11 8.544128111861365 1102 12 5.9797990115946185 1102 13 10.462108238278667 1102 14 7.974128795081338 1102 15 5.8756930896190305 1102 16 6.341935077139787 1102 17 7.085635335341209 1102 18 7.038607650407837 1102 19 5.840342908013088 1102 20 6.3803557671715865 1102 21 5.229111874784159 1102 22 6.32011337264478 1102 23 6.7161471440117175 1102 24 8.10114525845643 1102 25 7.77027111465565 1103 1 5.411945363775104 1103 2 9.733448132405956 1103 3 6.7632019880633365 1103 4 8.352706390287548 1103 5 7.640440043610486 1103 6 7.077068068353867 1103 7 7.092284008167049 1103 8 6.534286680488815 1103 9 5.596993540042902 1103 10 4.965898838082367 1103 11 5.953398461193913 1103 12 4.832570911157585 1103 13 8.704502494667832 1103 14 7.02461528280277 1103 15 5.909516385246114 1103 16 5.83534195717375 1103 17 3.4250510741066797 1103 18 2.19381156020604 1103 19 4.405522552921319 1103 20 4.722177052306417 1103 21 6.950409765575973 1103 22 2.9678431442083095 1103 23 4.90428476372977 1103 24 6.337339545117214 1103 25 2.673718461557904 1104 1 5.516937368119912 1104 2 10.067625032505527 1104 3 6.878990012683918 1104 4 7.214157620562414 1104 5 7.207778452173379 1104 6 6.87459460110588 1104 7 6.855851635569515 1104 8 6.75576067198023 1104 9 5.718450653913099 1104 10 4.682953061447783 1104 11 7.590778526366048 1104 12 4.539149223320031 1104 13 8.641573482508402 1104 14 6.453368149169727 1104 15 5.744205271285399 1104 16 5.2826323918055 1104 17 5.631136729448681 1104 18 3.5343284114265074 1104 19 4.299185053298473 1104 20 3.484020145116361 1104 21 2.548426786515538 1104 22 4.610222851084572 1104 23 4.872737283869771 1104 24 6.030733512169082 1104 25 5.103385453833798 1105 1 3.464084199741542 1105 2 9.255889009774888 1105 3 6.151008466527113 1105 4 5.476173614139828 1105 5 2.2790008343026003 1105 6 4.837028839866353 1105 7 5.619792370911512 1105 8 3.029384772965816 1105 9 3.9406325396975745 1105 10 3.411738818543707 1105 11 6.456379571146584 1105 12 3.7215085627109232 1105 13 5.06158802212423 1105 14 3.75400060396003 1105 15 4.339708904339531 1105 16 4.4518140482118085 1105 17 5.209566025479699 1105 18 2.442318170801414 1105 19 2.4334369475181425 1105 20 1.6763070269052136 1105 21 2.857739077485437 1105 22 4.214913172066016 1105 23 1.629767077133451 1105 24 4.572257434091714 1105 25 5.023202524748648 1106 1 6.358602097252671 1106 2 10.91757271909004 1106 3 7.583026864578827 1106 4 8.918561392940934 1106 5 8.500555567960404 1106 6 7.497905313730854 1106 7 7.201552490021967 1106 8 6.87833391076191 1106 9 6.0552945564938945 1106 10 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1.2498980173907697 2204 6 4.010464166323274 2204 7 5.284520113830386 2204 8 5.009836758936753 2204 9 3.6545919582609265 2204 10 3.092599800578217 2204 11 4.545941569844306 2204 12 4.746350310276554 2204 13 3.214943047725793 2204 14 4.049156192647985 2204 15 4.263906358241918 2204 16 4.288746522240281 2204 17 -1.0461730531600146 2204 18 2.5411218896873784 2204 19 3.4812502706897774 2204 20 3.725867971203318 2204 21 5.447883308254667 2204 22 4.692423938041095 2204 23 3.6798025012610758 2204 24 5.317418294777777 2204 25 5.347271323399017 2205 1 5.844845554983927 2205 2 10.244949269323287 2205 3 7.090194915551635 2205 4 7.533118652014775 2205 5 7.729271861054045 2205 6 7.519960581679424 2205 7 7.432907024098654 2205 8 7.162559931924726 2205 9 5.887261361591175 2205 10 5.4285734361008835 2205 11 7.516050265496482 2205 12 6.60711291434686 2205 13 9.78628902367399 2205 14 7.288445623730318 2205 15 6.174073448898717 2205 16 5.863995134636209 2205 17 6.429052432501411 2205 18 6.2681975141092074 2205 19 4.9386103539460215 2205 20 5.207641213571495 2205 21 -0.7618906279155446 2205 22 3.606392485682168 2205 23 5.041519083129714 2205 24 6.814874353982216 2205 25 7.0311866795507285 2206 1 3.939540825427404 2206 2 8.57781797820135 2206 3 5.874730219889553 2206 4 1.6519459114069512 2206 5 5.543395376391992 2206 6 5.661531297165482 2206 7 5.80095723914323 2206 8 5.945188673842754 2206 9 4.774792870605957 2206 10 4.045760268537009 2206 11 3.458361175804381 2206 12 4.768625097832576 2206 13 7.751562351025953 2206 14 0.4679063745349552 2206 15 2.4745742108116175 2206 16 3.341621063885121 2206 17 4.202828387384773 2206 18 -0.8196236286177268 2206 19 1.2325289360759584 2206 20 2.437316118866434 2206 21 3.2591910391783614 2206 22 -1.7896838409007607 2206 23 -2.431298606066786 2206 24 1.611507695860368 2206 25 2.9325581227704163 2207 1 1.1057627972210433 2207 2 -13.785635837059692 2207 3 -6.258856856522469 2207 4 -4.342645015344527 2207 5 0.8988060148191543 2207 6 1.5175762932893626 2207 7 2.5321746350910743 2207 8 2.849105119082676 2207 9 0.4992698707661596 2207 10 0.05538611718607811 2207 11 -1.919945264077107 2207 12 -2.509464794980307 2207 13 0.1711526793267737 2207 14 -5.337020807830456 2207 15 -1.7655821965693974 2207 16 -0.488917044532041 2207 17 0.4490705776036954 2207 18 0.532280088957755 2207 19 -0.10913943387334868 2207 20 -0.14334121933552524 2207 21 -3.6776540026119093 2207 22 -4.330808449375926 2207 23 -1.4825167753970199 2207 24 0.37185582841810927 2207 25 0.6255777943038652 2208 1 4.705820087860239 2208 2 6.494652309633169 2208 3 6.061759936661026 2208 4 7.754523872235204 2208 5 3.4681119243125558 2208 6 5.921555020085979 2208 7 6.2458119691306315 2208 8 6.323876969921631 2208 9 4.951547038554768 2208 10 4.342144232684365 2208 11 6.193870337533156 2208 12 5.54051948612576 2208 13 8.309751887166584 2208 14 2.8985549675974447 2208 15 4.35876191118745 2208 16 4.954094533401694 2208 17 5.653578342016662 2208 18 0.5873664798186375 2208 19 3.102747202724739 2208 20 4.180195955173023 2208 21 6.5495430168335815 2208 22 5.857340405396808 2208 23 1.3985565052757671 2208 24 2.967990601944429 2208 25 4.549659139805154 2209 1 2.6877593336391534 2209 2 4.551480317813473 2209 3 4.786761969495899 2209 4 0.5102326408793587 2209 5 4.745547107481908 2209 6 5.141124793536367 2209 7 5.580383801646022 2209 8 4.1881596677426955 2209 9 3.8377174661845324 2209 10 2.941020883761592 2209 11 -0.946107611673078 2209 12 2.627927013997457 2209 13 2.0098344246341355 2209 14 3.2332177312211563 2209 15 3.7008884575627548 2209 16 3.225506404982486 2209 17 0.8450823163181305 2209 18 2.9431488676870288 2209 19 3.5536094307292494 2209 20 3.8306589606385053 2209 21 5.896251966465616 2209 22 4.423186331377135 2209 23 0.09236435910051455 2209 24 -2.906285774415931 2209 25 0.05187383689527181 2210 1 -1.8642840775246974 2210 2 -13.785635837059692 2210 3 -7.399407388302436 2210 4 -1.877572300619999 2210 5 -0.2519991453274528 2210 6 -0.8789297816661978 2210 7 -0.7368152844934173 2210 8 -20.562174110628465 2210 9 -12.753021937687759 2210 10 -6.800572871958163 2210 11 -9.484406415088069 2210 12 -4.301025809205459 2210 13 -5.377668560092426 2210 14 -3.9713551950558674 2210 15 -5.5318746884737635 2210 16 -4.848781464810035 2210 17 -4.443569311972151 2210 18 -4.565116386007759 2210 19 -9.708107653427497 2210 20 -6.7072510153629 2210 21 -4.960900181654868 2210 22 -8.899832158597434 2210 23 -7.358836174573241 2210 24 -9.982383526649715 2210 25 -7.665718071778134 2211 1 -0.017696342422077294 2211 2 4.647240875269075 2211 3 0.5792505844528293 2211 4 1.4275863727958864 2211 5 0.9183967517564646 2211 6 0.047377879843224946 2211 7 -20.55379510356092 2211 8 -12.841626165422985 2211 9 -9.051726907135144 2211 10 -7.26270097369218 2211 11 -9.617254529024066 2211 12 -4.944725106638277 2211 13 -6.476231014215838 2211 14 -4.942853560181078 2211 15 -5.276219832943308 2211 16 -11.439894037616778 2211 17 -7.761431391456618 2211 18 -5.855844310431742 2211 19 -8.589949103937979 2211 20 -6.727022514204663 2211 21 -4.445738267087798 2211 22 -6.03007829543121 2211 23 -8.29828527420051 2211 24 -21.802146922613527 2211 25 -21.63370693747055 2212 1 -1.0584289542437695 2212 2 -13.785635837059692 2212 3 -7.713025961373251 2212 4 -19.419673901176715 2212 5 -20.000781330435316 2212 6 -20.730704311937597 2212 7 -9.805866926765338 2212 8 -9.41783709405388 2212 9 -5.284255477482528 2212 10 -5.377343749746068 2212 11 -5.482741170967607 2212 12 -2.7909012210710316 2212 13 -6.3169825467973855 2212 14 -3.7786535299078636 2212 15 -3.8280608228774646 2212 16 -3.9982910708416313 2212 17 -3.9664453928189154 2212 18 -3.054970171326314 2212 19 -3.6547318936148123 2212 20 -3.7696017921884675 2212 21 -1.7063811945077632 2212 22 -2.9275391294583066 2212 23 -3.068136935443224 2212 24 -2.1785281380831414 2212 25 -2.5121599761445808 2213 1 -18.602627849271393 2213 2 -13.785635837059692 2213 3 -8.70213079986063 2213 4 -6.0839647065936795 2213 5 -20.000781330435316 2213 6 -10.907468388915502 2213 7 -20.55379510356092 2213 8 -10.677640967364033 2213 9 -8.658698758061242 2213 10 -7.457827854446727 2213 11 -6.6301320224579 2213 12 -5.458445217164645 2213 13 -19.02554608270899 2213 14 -12.414437445983518 2213 15 -7.470847294644752 2213 16 -6.6773529075530185 2213 17 -5.2930324615705615 2213 18 -5.379962393990457 2213 19 -6.114949836223982 2213 20 -6.198234398718277 2213 21 -4.346274300440985 2213 22 -5.19043867778214 2213 23 -5.016952220763159 2213 24 -4.092239581202264 2213 25 -4.833992040820515 2214 1 -18.602627849271393 2214 2 -13.785635837059692 2214 3 -12.466486990550003 2214 4 -19.419673901176715 2214 5 -20.000781330435316 2214 6 -20.730704311937597 2214 7 -16.697596756453482 2214 8 -15.051430308975572 2214 9 -14.299202283564442 2214 10 -14.275007098451606 2214 11 -14.737927007230791 2214 12 -12.839336224038933 2214 13 -19.02554608270899 2214 14 -16.934560794416335 2214 15 -23.520740380888515 2214 16 -18.39497158771301 2214 17 -23.273075227073058 2214 18 -17.995621283147692 2214 19 -15.364561353456828 2214 20 -14.185128480467542 2214 21 -11.80983879918335 2214 22 -12.7867464715888 2214 23 -12.678195836863251 2214 24 -11.624460972200303 2214 25 -11.42957470606559 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 161771.0 3 6.0 4 791.0 5 2975.0 6 4130.0 7 4064.0 8 3341.0 9 3091.0 10 2863.0 11 2680.0 12 2523.0 13 2736.0 14 4039.0 15 8708.0 16 12744.0 17 13136.0 18 13744.0 19 16009.0 20 19011.0 21 22176.0 22 23228.0 23 24904.0 24 26124.0 25 26897.0 26 22373.0 27 17689.0 28 18643.0 29 21558.0 30 21152.0 31 24033.0 32 30527.0 33 38723.0 34 47487.0 35 54266.0 36 54455.0 37 57569.0 38 44360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27325561750296 18.718702483527622 12.505896266261193 25.502145632708228 2 13.790755464437904 19.68696398735126 42.72558557791762 23.79669497029322 3 19.132480670999545 23.835166855484665 29.310764613519368 27.721587859996422 4 11.189124243624127 17.073854397559458 38.885918039947256 32.85110331886916 5 12.314159676176626 37.46622318202043 37.05316932359548 13.16644781820747 6 32.01171103059548 37.637522670842095 17.996620546909536 12.354145751652888 7 27.905964639595883 31.09202771841202 22.452561362606048 18.54944627938605 8 24.149118030669488 38.13944359321776 20.840814982715177 16.870623393397576 9 25.609684660408927 16.040423826907666 20.32180665357633 38.028084859107075 10 13.568183572756265 30.311121140678537 35.11339679674092 21.00729848982428 11 33.24864330298573 22.915507096568998 24.683934321949668 19.151915278495608 12 22.61788350862526 25.29092985586002 32.27262435276349 19.818562282751227 13 27.86321049519213 21.650257419193917 25.856265642034575 24.630266443579373 14 19.60181617691666 22.636311405110465 28.53864847793478 29.22322394003809 15 22.081374716686632 31.73447490980412 23.811591633103305 22.372558740405946 16 21.152038534817805 29.35058165421227 27.988541876569368 21.508837934400553 17 20.75703076861859 29.070478308105805 28.91179389354398 21.260697029731624 18 20.4036641860045 28.516166295478477 30.62064883854146 20.459520679975565 19 22.169377656251115 27.796888400037062 27.972922571096664 22.060811372615163 20 22.147764427534312 28.338571458749467 28.958579298333735 20.555084815382486 21 21.614337905027554 26.99043951747812 27.54990115973396 23.845321417760363 22 21.425639768873047 28.586316304157737 28.935481223017046 21.052562703952166 23 21.194089653378697 28.309046644969154 28.78751666561225 21.7093470360399 24 21.436433327232844 27.705975300749845 29.393061423338757 21.464529948678557 25 21.783279049605174 27.95311291796518 28.791386461091456 21.47222157133819 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 912.0 1 912.0 2 2012.0 3 3112.0 4 3112.0 5 3112.0 6 4372.5 7 5633.0 8 5633.0 9 5633.0 10 6758.5 11 7884.0 12 7884.0 13 7884.0 14 9886.0 15 11888.0 16 11888.0 17 11888.0 18 16477.5 19 21067.0 20 21067.0 21 21067.0 22 28954.0 23 36841.0 24 36841.0 25 36841.0 26 46884.5 27 56928.0 28 56928.0 29 56928.0 30 67767.0 31 78606.0 32 78606.0 33 78606.0 34 86918.0 35 95230.0 36 95230.0 37 95230.0 38 99727.5 39 104225.0 40 104225.0 41 104225.0 42 105032.5 43 105840.0 44 105840.0 45 105840.0 46 103211.5 47 100583.0 48 100583.0 49 100583.0 50 92772.5 51 84962.0 52 84962.0 53 84962.0 54 72990.5 55 61019.0 56 61019.0 57 61019.0 58 50141.0 59 39263.0 60 39263.0 61 39263.0 62 30822.0 63 22381.0 64 22381.0 65 22381.0 66 16789.0 67 11197.0 68 11197.0 69 11197.0 70 7886.0 71 4575.0 72 4575.0 73 4575.0 74 3119.5 75 1664.0 76 1664.0 77 1664.0 78 1090.5 79 517.0 80 517.0 81 517.0 82 337.0 83 157.0 84 157.0 85 157.0 86 98.0 87 39.0 88 39.0 89 39.0 90 21.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.416911831822553 2 36.754528241387625 3 5.794791498444752 4 13.996882482218211 5 13.730887064875732 6 15.02271434690109 7 9.651666537940331 8 7.585023744157579 9 0.11351322253506622 10 1.4711079592663066 11 5.807898179809626 12 5.935922370998659 13 16.489141348537085 14 1.6218347949623533 15 2.9837594175016324 16 3.015589929387754 17 2.9498224746818704 18 1.5311412408750582 19 1.479650706941626 20 0.2855384154490326 21 4.512794227443051 22 1.6323669496305555 23 0.11374727041658181 24 9.201826509667347 25 0.11538560558719103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 854526.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.61001540163407 #Duplication Level Percentage of deduplicated Percentage of total 1 97.47274401202597 92.21897812217313 2 1.878613264230945 3.5547125972520752 3 0.3133624640207086 0.8894168264187973 4 0.1230840184401913 0.4657992352128604 5 0.055376475791965044 0.2619584613783014 6 0.03956248302759081 0.2245804277140352 7 0.023205777698754478 0.15368492898402403 8 0.013647002640978362 0.1032914504039283 9 0.012429186411907394 0.10583329660743056 >10 0.05977689336417936 0.9822579909557471 >50 0.004793644758407617 0.3058744430848044 >100 0.0031525718782593774 0.6002281893717663 >500 2.5220571019344537E-4 0.13338403044312097 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.1702394075780023E-4 0.0 0.0 0.0 6 0.0 1.1702394075780023E-4 0.0 0.0 0.0 7 0.0 1.1702394075780023E-4 0.0 0.0 0.0 8 1.1702394075780023E-4 1.1702394075780023E-4 0.0 0.0 0.0 9 1.1702394075780023E-4 1.1702394075780023E-4 0.0 0.0 0.0 10 1.1702394075780023E-4 1.1702394075780023E-4 0.0 0.0 0.0 11 1.1702394075780023E-4 1.1702394075780023E-4 0.0 0.0 0.0 12 1.1702394075780023E-4 1.1702394075780023E-4 0.0 0.0 0.0 13 1.1702394075780023E-4 1.1702394075780023E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 425 0.0 17.13111 2 ACGTGCA 35 0.0017928634 16.755758 8 CTGTAGG 460 0.0 15.436076 1 CATAAGG 55 0.0037727952 14.986048 2 GTCTTAC 65 8.0939964E-4 14.427916 1 TCCTACA 485 0.0 14.16207 2 GTAGGAC 475 0.0 14.105358 3 TAGGACC 210 0.0 13.833048 4 GTTTAGC 60 0.0079330895 13.397349 1 GTCCTAG 110 1.4982979E-6 13.397349 1 GTCCTAC 430 0.0 13.397349 1 GTATAGT 70 0.0014168636 13.397349 1 GTGTTAC 80 2.5417583E-4 13.397348 1 TAGGACG 395 0.0 13.074272 4 GGACCTG 285 0.0 12.839845 6 TCCTAGC 75 0.0019722916 12.821395 2 TGCTATA 75 0.0019722916 12.821395 2 AGGACGT 370 0.0 12.71797 5 GTTTTAC 85 4.242333E-4 12.60927 1 CTAGGAC 105 6.008886E-7 12.552777 3 >>END_MODULE