FastQCFastQC Report
Thu 26 May 2016
SRR1926642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854526
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16090.18829152067930058No Hit
CTGTAGGACGTGGAATATGGCAAGA11970.1400776570870869No Hit
GTCCTACAGTGGACATTTCTAAATT11240.13153490941176746No Hit
GTCCTACAGTGTGCATTTCTCATTT10840.12685395178145545No Hit
GGTATCAACGCAGAGTACTTTTTTT10370.12135382656583885No Hit
CACACACACACACACACACACACAC9330.10918333672702762No Hit
CTGTAGGACCTGGAATATGGCGAGA8930.10450237909671561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT402.803798E-416.59015811
TCCAACG753.3996912E-916.42061618
TAGGACC3300.014.4534884
CCAACGA552.006597E-413.77873319
AACTGAT604.0922785E-412.6653377
ACCGTGC604.0943047E-412.6645948
GTAGGAC7000.012.4018243
TGTAGGA7300.012.2878292
TGCCGGT701.1107341E-412.18940110
CCGGTTT550.003115056312.0655712
GGCGAGA4000.011.60427619
ACTCTCC1157.071867E-811.5633258
GGACCTG5300.011.5191576
CTGTAGG7450.011.393931
GTATTAG1104.7105095E-711.27172851
TGGCGAG5800.011.2701218
TCTAGCC600.00569171511.1298423
GTCCTAC5750.011.1133971
CTTATCC951.2964712E-511.0461593
TAGAGTG1303.0642695E-811.00688655
Produced by FastQC (version 0.11.5)