FastQCFastQC Report
Thu 26 May 2016
SRR1926641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924578
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA5400.017.2169652
TAGGACC2350.016.4362244
GTAGGAC5450.015.8659973
GGACGTT400.00285498115.6280976
CTGTAGG5400.015.3078051
GTCCTAC5800.014.711841
GGACCTG3350.014.6173246
GTCCTAA3100.014.1927711
TCCTACA6950.013.96738152
TCTATAC1201.207809E-913.6819133
GGATAAG600.00819254613.3326031
ACACCGT550.001532212413.2602036
GCACCAT550.001532212413.2602036
TTTGGCG503.003217E-413.13797216
TTAGTAC755.5178534E-513.1346363
TCCTAAA3250.013.0412662
TGTTAGG1051.10947185E-513.02123452
AGGACCT3900.012.8297355
TAGGACA2050.012.5610174
CTAGGGT600.00236359412.5174034
Produced by FastQC (version 0.11.5)