FastQCFastQC Report
Thu 26 May 2016
SRR1926641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924578
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18080.1955486719346556No Hit
GTCCTACAGTGGACATTTCTAAATT17310.18722054818522613No Hit
GTATCAACGCAGAGTACTTTTTTTT16700.1806229436564573No Hit
CTTTAGGACGTGAAATATGGCGAGG12970.14028021432480547No Hit
GTCCTACAGTGTGCATTTCTCATTT12470.13487234176024088No Hit
GTCCTAAAGTGTGTATTTCTCATTT12380.13389892469861928No Hit
GTACATGGGAAGCAGTGGTATCAAC10480.11334900895327382No Hit
CCCATGTACTCTGCGTTGATACCAC10080.10902271090162215No Hit
CTGTAGGACCTGGAATATGGCGAGA9910.1071840342296702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC453.594801E-516.8566510
TAGGACC3350.013.9482794
TCCAACG802.0613752E-613.025578518
GTAGGAC8750.012.642793
TGTAGGA9750.012.1318862
GCGTGCG550.00305967912.0946729
GTCCTAC8500.011.8920381
AAGGCGT657.77059E-411.736066
AGGACCT6150.011.6287195
CTGTAGG10200.011.4993761
TATAGTG1501.6370905E-1011.4426585
GACGTGG4250.011.4016927
TTAGGAC4900.011.2882053
GATCTAC855.115789E-511.21890451
TAGGACG8500.011.21890454
AGGACGT8450.011.1718265
ACAGTCC951.3537434E-511.0034248
TAGGACA2950.010.9907234
GCTACAC1657.4578566E-1110.9815243
GGACGTG8250.010.98032956
Produced by FastQC (version 0.11.5)