##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926641_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 924578 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.09038934519316 31.0 28.0 31.0 16.0 34.0 2 28.568752447062334 31.0 28.0 33.0 22.0 34.0 3 28.93141736013619 31.0 28.0 33.0 23.0 34.0 4 32.672619292260904 35.0 33.0 37.0 26.0 37.0 5 32.316314037322975 35.0 32.0 37.0 25.0 37.0 6 32.39540092885619 35.0 33.0 37.0 25.0 37.0 7 32.205996681729395 35.0 33.0 37.0 22.0 37.0 8 32.15891790633132 35.0 33.0 37.0 21.0 37.0 9 33.350307924263824 37.0 33.0 39.0 18.0 39.0 10 33.23795288228792 37.0 33.0 39.0 18.0 39.0 11 33.483583862043005 37.0 33.0 39.0 22.0 39.0 12 33.2835747768171 37.0 33.0 39.0 18.0 39.0 13 33.41266826595485 37.0 33.0 39.0 21.0 39.0 14 34.04502811012159 38.0 33.0 40.0 17.0 41.0 15 34.16577400716868 38.0 33.0 40.0 18.0 41.0 16 34.146213732102645 38.0 33.0 40.0 18.0 41.0 17 34.05701087415015 38.0 33.0 40.0 17.0 41.0 18 34.01353698660362 38.0 33.0 40.0 17.0 41.0 19 34.05403221794159 39.0 33.0 40.0 18.0 41.0 20 34.03707745587717 39.0 34.0 40.0 18.0 41.0 21 33.952313379725666 39.0 34.0 40.0 16.0 41.0 22 33.87377268332147 39.0 34.0 40.0 11.0 41.0 23 33.52628009751476 39.0 33.0 40.0 10.0 41.0 24 33.612714124714195 39.0 33.0 40.0 2.0 41.0 25 33.39763762494889 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6713666217268646 1101 2 0.8644131085515809 1101 3 0.9360282203451575 1101 4 0.6657970870906524 1101 5 0.8665772569014294 1101 6 0.3491994156534588 1101 7 0.5622918243143289 1101 8 0.6724312020733691 1101 9 1.0158334908861093 1101 10 0.8855081484041776 1101 11 0.8010993140033662 1101 12 0.8412598112769629 1101 13 0.7049988496167501 1101 14 1.034582836484553 1101 15 0.9043230004527985 1101 16 0.9231132555066139 1101 17 0.8621177786145964 1101 18 0.8619216538588148 1101 19 0.9387525352549915 1101 20 1.1493114556557202 1101 21 1.0215345804736273 1101 22 1.0318712387510587 1101 23 1.1996270562613773 1101 24 1.0889948139869574 1101 25 1.121516081231789 1102 1 0.7250761538922355 1102 2 0.565241871397685 1102 3 0.5298698394904662 1102 4 0.320523772156335 1102 5 0.4304116948616823 1102 6 -0.06120066798575152 1102 7 0.2588219677321604 1102 8 0.6019239940446468 1102 9 0.5635145009506672 1102 10 0.5336627776043557 1102 11 0.3782805030929737 1102 12 0.37588914869861156 1102 13 0.3216583916113436 1102 14 0.7096231120741336 1102 15 0.6689968983588699 1102 16 0.4925780008934382 1102 17 0.35383882893254537 1102 18 0.465036649295385 1102 19 0.46689337528813724 1102 20 0.5954830444010781 1102 21 0.5805198011184629 1102 22 0.49703280330482613 1102 23 0.6078917565992796 1102 24 0.6605533652686546 1102 25 0.6304332239193968 1103 1 -0.048255385451366095 1103 2 -0.030931709158142695 1103 3 -0.3206849250584227 1103 4 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0.8426023603519184 2202 16 0.7245094370834195 2202 17 0.6393706534128398 2202 18 0.5783374730518389 2202 19 0.5479873904396229 2202 20 0.48514401462268353 2202 21 0.5948286031524219 2202 22 0.8545387823113799 2202 23 0.9168178321902474 2202 24 0.7331393251167739 2202 25 0.7182614990885412 2203 1 -0.30424382207847245 2203 2 -0.18338404683790444 2203 3 -0.20967654527236945 2203 4 -0.6440832882351337 2203 5 -0.3867014497104151 2203 6 -0.620459216373785 2203 7 -0.38670648147272146 2203 8 -0.14500815809767076 2203 9 0.04890374310562606 2203 10 0.2039662429907878 2203 11 -0.025522140960234196 2203 12 0.037559267706761545 2203 13 -0.1382055420970545 2203 14 0.2586725341496816 2203 15 0.17047380256823885 2203 16 0.048850336213426715 2203 17 0.13730846951513342 2203 18 0.2007127556856716 2203 19 0.195268880364452 2203 20 0.189701639518276 2203 21 0.1752788891685455 2203 22 0.24670896453373814 2203 23 0.5622817972380361 2203 24 0.33934726422098294 2203 25 0.18314644858913454 2204 1 0.46713069825733555 2204 2 0.430448112829513 2204 3 0.16839287810470793 2204 4 -0.0652901245643065 2204 5 0.17434505481693208 2204 6 0.023877265461869968 2204 7 0.23572604800327213 2204 8 0.156046309282722 2204 9 0.5829400546925498 2204 10 0.4062813325973096 2204 11 0.19947975204930657 2204 12 0.3859182116272706 2204 13 0.25529984748430934 2204 14 0.7107036723247191 2204 15 0.44360698983451385 2204 16 0.44603549208749627 2204 17 0.3888100535366661 2204 18 0.2895913856841261 2204 19 0.3264446713371143 2204 20 0.364270094407253 2204 21 0.39051773812103363 2204 22 0.3720445157414787 2204 23 0.4960728724918795 2204 24 0.5995163893574613 2204 25 0.5104648967609791 2205 1 0.5204292463778408 2205 2 0.4476241755350401 2205 3 0.28358491666791963 2205 4 0.02911486976294242 2205 5 0.06363708358959741 2205 6 -0.1736785550141775 2205 7 -0.24245083825945102 2205 8 -0.08769667176394336 2205 9 0.16519498883677386 2205 10 0.15323503486123258 2205 11 -0.15121854382081068 2205 12 -0.08710309143602046 2205 13 -0.1903920269433712 2205 14 0.24833447681915288 2205 15 0.1958429764041938 2205 16 0.03450223592665225 2205 17 0.041424415682982385 2205 18 0.04676756357248024 2205 19 0.09150396169573582 2205 20 0.1881083316493104 2205 21 0.05414703372047569 2205 22 0.17387016350088658 2205 23 0.5225050269290108 2205 24 0.39118867118679646 2205 25 0.4125030568305519 2206 1 0.25865554607203123 2206 2 0.34854160672770007 2206 3 0.5490283417443713 2206 4 0.3838549309250183 2206 5 0.7015575728862373 2206 6 0.6636303134873529 2206 7 0.6119834125050758 2206 8 0.698847670743703 2206 9 0.8257454475523716 2206 10 0.6780056770630623 2206 11 0.4683533216226152 2206 12 0.41259109816643047 2206 13 0.40899635226151787 2206 14 0.6727993086234321 2206 15 0.5964545971992976 2206 16 0.5996398506055485 2206 17 0.5056445991692229 2206 18 0.4284189397192719 2206 19 0.44624402285781883 2206 20 0.49453034999793744 2206 21 0.6828932110905086 2206 22 0.6949710809320777 2206 23 0.9952879015467531 2206 24 0.6431764387708938 2206 25 0.9788639130996657 2207 1 -0.9797324044985523 2207 2 -0.10512648423503279 2207 3 -0.06725822431817008 2207 4 0.07869382785480639 2207 5 0.17676963154185898 2207 6 0.33925255403384824 2207 7 0.417217748612039 2207 8 0.4285170456872365 2207 9 0.6581551988868029 2207 10 0.42084931011365256 2207 11 0.34294722425150326 2207 12 0.13023564691829392 2207 13 0.09265000805086743 2207 14 -4.7944301568138386 2207 15 -1.8228593188075948 2207 16 -0.6796459639053509 2207 17 -0.5987995505490957 2207 18 -0.2726780181304065 2207 19 0.047737899922424276 2207 20 0.333861680474989 2207 21 0.26196624614818376 2207 22 0.18058107752991504 2207 23 0.5600872218826609 2207 24 0.314329349736056 2207 25 0.27135704630114077 2208 1 0.3233600200454987 2208 2 0.6639072188382293 2208 3 0.6057131829079729 2208 4 0.5686667106109411 2208 5 0.5468991337163871 2208 6 0.5224318849827725 2208 7 0.5554008709050677 2208 8 0.6902730083866757 2208 9 1.0250935599077593 2208 10 0.9639558114672795 2208 11 0.7644626733263351 2208 12 0.6900579868449128 2208 13 0.6218948036202505 2208 14 0.9645935776712875 2208 15 0.9045068788060533 2208 16 0.8159065695102896 2208 17 0.6971374109924398 2208 18 0.7919839706812155 2208 19 0.6740988880726988 2208 20 0.5678051331555096 2208 21 0.816337550333273 2208 22 0.7648395062806017 2208 23 1.203737410182356 2208 24 0.9723307261020722 2208 25 0.9811395342430203 2209 1 -0.5245496264492324 2209 2 -0.19383268328784453 2209 3 0.17022842561084417 2209 4 0.41647634369727626 2209 5 0.49543985382758393 2209 6 0.4130637425871271 2209 7 0.7077502334936057 2209 8 0.6399794525473226 2209 9 0.44169556589098136 2209 10 0.47094502687124873 2209 11 0.533428036448278 2209 12 0.5491563128475931 2209 13 0.5802452637918378 2209 14 0.7555974265389409 2209 15 0.7056963423018985 2209 16 0.5988888301357065 2209 17 0.6227380592844796 2209 18 0.6227711751196097 2209 19 0.6615232094286014 2209 20 0.37040797295014727 2209 21 0.6177138520645684 2209 22 0.6339582232954157 2209 23 0.9710520025416258 2209 24 0.8864364267198113 2209 25 0.8325573433705458 2210 1 -0.3323987852892536 2210 2 -0.5193368693647855 2210 3 0.2042342636055281 2210 4 0.2602732338202145 2210 5 0.38048523586603267 2210 6 0.2615267952387583 2210 7 0.6740314847563553 2210 8 0.4650770571222793 2210 9 0.8807570226188162 2210 10 0.775087617101363 2210 11 0.4172115342037088 2210 12 0.3415230482078684 2210 13 0.07938418843860262 2210 14 0.47830961186159726 2210 15 0.4135056703937323 2210 16 0.4745433917184698 2210 17 0.34813795422366667 2210 18 0.41135071827824987 2210 19 0.23269690805030763 2210 20 0.5245683619445458 2210 21 0.5712981383533631 2210 22 0.6578451270608596 2210 23 0.8337171221589443 2210 24 0.5038126387194524 2210 25 0.6322864173152709 2211 1 -1.5643680790901335 2211 2 -0.6309301189000642 2211 3 0.0413369290632275 2211 4 0.2572385668690842 2211 5 0.4710222980350238 2211 6 0.1957838431055805 2211 7 0.43231854346832677 2211 8 0.3336034005678741 2211 9 0.6135921506370394 2211 10 0.5137139374343391 2211 11 0.2946312968746554 2211 12 0.48526654161089766 2211 13 0.4346231472250537 2211 14 0.6565861217377957 2211 15 0.2580754892658774 2211 16 0.49633760250159753 2211 17 0.6441714734456596 2211 18 0.3589858233813956 2211 19 0.6312133545544754 2211 20 0.465704277964754 2211 21 0.4252651129907221 2211 22 0.5508988964917592 2211 23 0.7641607856471282 2211 24 0.794912305463157 2211 25 0.7467586693336798 2212 1 1.3611802016444692 2212 2 0.23055132511947818 2212 3 0.6455456053354247 2212 4 0.6620060600470481 2212 5 0.8430721160780479 2212 6 0.8521058267672785 2212 7 0.9470127740555156 2212 8 0.9403403256657441 2212 9 1.208733884292144 2212 10 0.8372234528396305 2212 11 0.9269338360594901 2212 12 0.9599942113037656 2212 13 0.9629471849538405 2212 14 0.9124220799285396 2212 15 0.8664307702542118 2212 16 0.5030025142203698 2212 17 1.0056652366807128 2212 18 0.9985558352121657 2212 19 0.8018258443370883 2212 20 0.7692281355304189 2212 21 1.0400735016416505 2212 22 1.2077944801178333 2212 23 1.3149892204713467 2212 24 1.2851656447270088 2212 25 0.9838819552877425 2213 1 -0.11245473290194496 2213 2 -0.18594631688210939 2213 3 -0.656189158099604 2213 4 -1.5371773351924354 2213 5 -2.3482832808864664 2213 6 -1.1149879227979902 2213 7 -0.8327585209655588 2213 8 -0.7386421875849507 2213 9 -1.094244566801997 2213 10 -1.2553338972987902 2213 11 -1.2064454052075675 2213 12 -1.1614587052411736 2213 13 -1.4172971008995603 2213 14 -7.822682186727 2213 15 -3.764077138430732 2213 16 -2.475726809820088 2213 17 -1.7187703044068883 2213 18 -5.621696519308376 2213 19 -3.3303458791580347 2213 20 -5.375768595268244 2213 21 -2.7311241389687844 2213 22 -2.4970216794748374 2213 23 -5.285165197123913 2213 24 -3.034193591810144 2213 25 -2.3737978855647555 2214 1 0.3748611465628322 2214 2 0.1902629831330529 2214 3 -1.5783034919192183 2214 4 -1.9050623216235962 2214 5 -0.3989099573833528 2214 6 -0.5623915422011692 2214 7 -0.6097918861948202 2214 8 -0.639389911732259 2214 9 -5.170000003488585 2214 10 -1.7490320991006492 2214 11 -1.0022994578201576 2214 12 -1.3961572887483662 2214 13 -1.4851156810602966 2214 14 -2.5902314816178986 2214 15 -2.205426806660821 2214 16 -1.6446967805908699 2214 17 -1.7871196118470039 2214 18 -1.1395483026043252 2214 19 -3.7962925345304086 2214 20 -2.081117175899575 2214 21 -3.7576082145359813 2214 22 -6.0586059134758905 2214 23 -6.059932383014409 2214 24 -5.64718359949654 2214 25 -3.3937673491380416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35622.0 3 32.0 4 671.0 5 4179.0 6 5165.0 7 4322.0 8 3158.0 9 2520.0 10 2252.0 11 2118.0 12 1965.0 13 1924.0 14 1904.0 15 1953.0 16 1992.0 17 2175.0 18 2280.0 19 2850.0 20 3698.0 21 4685.0 22 5952.0 23 7275.0 24 8645.0 25 10286.0 26 11829.0 27 12812.0 28 14739.0 29 18020.0 30 21849.0 31 26994.0 32 34805.0 33 44353.0 34 57932.0 35 75639.0 36 101829.0 37 148503.0 38 237651.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.442013830252485 20.048453514503578 9.507540821761214 30.001991833482723 2 13.08039109826252 21.745941928161606 40.01886896563663 25.15479800793925 3 18.132118993214235 25.074760247888108 29.169263259936294 27.623857498961364 4 11.706785618845972 17.95675180922067 38.114429082552824 32.22203348938053 5 12.701682263211985 39.00860063828636 35.35673716665765 12.932979931843999 6 31.116009142187067 38.830950972035986 17.750717965700513 12.302321920076437 7 27.752778301610732 32.08664702834984 21.8289862423403 18.33158842769913 8 24.496047066678518 38.99844570705155 19.94437495528969 16.561132270980245 9 25.808259328028583 16.17526710739251 20.129768071548035 37.886705493030874 10 14.284045303592485 30.6241684064775 34.22346743398635 20.868318855943663 11 33.70057227407288 23.50671727108226 24.39114083826092 18.40156961658394 12 22.817194206008583 25.666815208362177 31.946451439844942 19.5695391457843 13 28.590773304145245 22.84425975958762 25.717246138238202 22.847720798028938 14 20.078108594326736 23.404110226640487 27.937352923984836 28.58042825504794 15 22.27488407961867 32.410549052140645 23.20971016389471 22.104856704345973 16 21.087094778466636 29.59658473905312 28.445004953729143 20.871315528751097 17 20.85010740453111 29.894261158497937 28.16735205106961 21.088279385901345 18 20.633042417346367 29.015273359717312 30.148039426705502 20.20364479623082 19 22.640233970247532 28.06734432087843 27.75173541633768 21.540686292536357 20 22.56759636111529 28.63637002754797 28.513069332845898 20.28296427849084 21 21.838851712729173 27.812718681083346 27.60083800483897 22.747591601348507 22 21.95617892703482 29.381836902889752 27.658239759111723 21.003744410963705 23 21.39440912502785 28.915137500567827 28.133375442634367 21.557077931769953 24 22.222294327345722 27.989802893434394 28.86620461703527 20.92169816218461 25 21.77360914925512 28.57303548213347 28.343741685395933 21.309613683215478 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 304.0 1 304.0 2 352.0 3 400.0 4 400.0 5 400.0 6 572.5 7 745.0 8 745.0 9 745.0 10 1312.5 11 1880.0 12 1880.0 13 1880.0 14 3199.5 15 4519.0 16 4519.0 17 4519.0 18 7821.0 19 11123.0 20 11123.0 21 11123.0 22 17830.5 23 24538.0 24 24538.0 25 24538.0 26 35112.5 27 45687.0 28 45687.0 29 45687.0 30 60243.0 31 74799.0 32 74799.0 33 74799.0 34 85401.5 35 96004.0 36 96004.0 37 96004.0 38 103918.0 39 111832.0 40 111832.0 41 111832.0 42 116842.0 43 121852.0 44 121852.0 45 121852.0 46 124083.5 47 126315.0 48 126315.0 49 126315.0 50 118674.5 51 111034.0 52 111034.0 53 111034.0 54 95380.5 55 79727.0 56 79727.0 57 79727.0 58 66743.5 59 53760.0 60 53760.0 61 53760.0 62 42981.0 63 32202.0 64 32202.0 65 32202.0 66 24339.0 67 16476.0 68 16476.0 69 16476.0 70 11888.5 71 7301.0 72 7301.0 73 7301.0 74 5044.5 75 2788.0 76 2788.0 77 2788.0 78 1859.0 79 930.0 80 930.0 81 930.0 82 593.0 83 256.0 84 256.0 85 256.0 86 167.5 87 79.0 88 79.0 89 79.0 90 50.5 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0871749057407812 2 0.03342065244900917 3 0.03136566087447462 4 0.030824873618018167 5 0.02465989889441453 6 0.38644657346378564 7 0.2046338978431241 8 0.21328649394642746 9 0.21728831964420522 10 0.016007302791111188 11 0.0022713064771171278 12 0.0036773533439039214 13 0.0 14 0.07960388415039077 15 0.0022713064771171278 16 0.00237946392840842 17 0.003893668246486505 18 0.002920251184864879 19 0.001730519220660669 20 9.734170616216263E-4 21 1.0815745129129181E-4 22 0.0 23 0.0 24 2.1631490258258363E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 924578.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.85729240741053 #Duplication Level Percentage of deduplicated Percentage of total 1 92.76548025419527 78.71827483244115 2 5.630334341211925 9.555498550874107 3 0.8286701534594586 2.109561165642091 4 0.27200575781683317 0.9232668851024922 5 0.1391498253999369 0.5903938711201282 6 0.07877973387509549 0.40110209479301767 7 0.048174651901993965 0.2861579366150902 8 0.0401751961812333 0.27273266959007975 9 0.029433840170737753 0.2247908383837152 >10 0.14077110206164475 2.2371318067241552 >50 0.013067543582822925 0.7610384702798764 >100 0.012036830128599267 2.1343177271351967 >500 8.963593400672518E-4 0.5853562423054187 >1k 0.0010244106743625735 1.2003769089934955 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1808 0.1955486719346556 No Hit GTCCTACAGTGGACATTTCTAAATT 1731 0.18722054818522613 No Hit GTATCAACGCAGAGTACTTTTTTTT 1670 0.1806229436564573 No Hit CTTTAGGACGTGAAATATGGCGAGG 1297 0.14028021432480547 No Hit GTCCTACAGTGTGCATTTCTCATTT 1247 0.13487234176024088 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1238 0.13389892469861928 No Hit GTACATGGGAAGCAGTGGTATCAAC 1048 0.11334900895327382 No Hit CCCATGTACTCTGCGTTGATACCAC 1008 0.10902271090162215 No Hit CTGTAGGACCTGGAATATGGCGAGA 991 0.1071840342296702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 45 3.594801E-5 16.85665 10 TAGGACC 335 0.0 13.948279 4 TCCAACG 80 2.0613752E-6 13.0255785 18 GTAGGAC 875 0.0 12.64279 3 TGTAGGA 975 0.0 12.131886 2 GCGTGCG 55 0.003059679 12.094672 9 GTCCTAC 850 0.0 11.892038 1 AAGGCGT 65 7.77059E-4 11.73606 6 AGGACCT 615 0.0 11.628719 5 CTGTAGG 1020 0.0 11.499376 1 TATAGTG 150 1.6370905E-10 11.442658 5 GACGTGG 425 0.0 11.401692 7 TTAGGAC 490 0.0 11.288205 3 GATCTAC 85 5.115789E-5 11.2189045 1 TAGGACG 850 0.0 11.2189045 4 AGGACGT 845 0.0 11.171826 5 ACAGTCC 95 1.3537434E-5 11.003424 8 TAGGACA 295 0.0 10.990723 4 GCTACAC 165 7.4578566E-11 10.981524 3 GGACGTG 825 0.0 10.9803295 6 >>END_MODULE