FastQCFastQC Report
Thu 26 May 2016
SRR1926640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849793
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2700.016.5837974
GTATAAT801.7085405E-514.8743891
TGTAGGA6000.014.6788692
CTGTAGG6150.014.6190911
GGACCTG4550.014.3391826
AGGACCT4950.014.2276535
GTAGGAC5750.014.1438163
GTCCTAC5750.013.7965341
TCTAGGA700.001301049813.5497262
CCTTATA853.85636E-412.7526832
GTATAAA1359.6446456E-812.7319871
TCCTACA6550.012.618832
TTATACT1101.107328E-712.599384
CAAGACT1101.107328E-712.599384
GTCTTAG854.742035E-412.4439331
GTGTAGA1605.2605174E-912.3953241
GACCTGG4800.012.0307247
TTATAGT801.2906712E-411.9936414
GTATAGG1001.3879726E-411.8995111
CCTACAG7450.011.7896783
Produced by FastQC (version 0.11.5)