FastQCFastQC Report
Thu 26 May 2016
SRR1926640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849793
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18530.21805310234374722No Hit
CTGTAGGACGTGGAATATGGCAAGA16540.19463563479576793No Hit
GTCCTACAGTGGACATTTCTAAATT15500.18239736029833148No Hit
CTTTAGGACGTGAAATATGGCGAGG12860.15133097118945438No Hit
GTCCTACAGTGTGCATTTCTCATTT12370.14556486108970068No Hit
GTCCTAAAGTGTGTATTTCTCATTT12360.14544718537337917No Hit
GGTATCAACGCAGAGTACTTTTTTT11310.13309123515962124No Hit
CTGTAGGACCTGGAATATGGCGAGA10250.12061760922954179No Hit
TATCAACGCAGAGTACTTTTTTTTT8720.10261322463235165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC400.005343147614.2230613
TAGGACC3250.013.20444
TGTAGGA10400.012.1054682
CTAGACC959.909018E-712.0461214
TAGACAG1750.011.9887585
GTAGGAC10350.011.9782273
TCTAGAC1052.582201E-711.807113
TCTACAC1302.4538167E-911.7372463
CTAGACT1156.689879E-811.6096664
TCCAACG1157.320705E-811.53452318
GGCGAGG3600.011.31710619
TAGGACT1701.0913936E-1111.2194254
CTAATAC600.0057065911.1259313
GCACCGT600.00570910711.1252726
GTCCTAA4350.010.9569651
CTGTAGG11050.010.9559741
AGGACGT9850.010.9403635
GACAGAT1053.4646964E-610.8557957
ATGCCGG700.00152359910.82832819
TAGGACG10250.010.792544
Produced by FastQC (version 0.11.5)