##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926640_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 849793 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.935874971904923 31.0 28.0 31.0 16.0 34.0 2 28.46200427633553 31.0 28.0 33.0 22.0 34.0 3 28.820313888205717 31.0 28.0 33.0 23.0 34.0 4 32.56957282538218 35.0 32.0 37.0 26.0 37.0 5 32.21998298409142 35.0 32.0 37.0 23.0 37.0 6 32.299607080783204 35.0 33.0 37.0 24.0 37.0 7 32.1335431099103 35.0 33.0 37.0 19.0 37.0 8 32.08320261522512 35.0 33.0 37.0 19.0 37.0 9 33.26607773893171 37.0 33.0 39.0 18.0 39.0 10 33.13934687623927 37.0 33.0 39.0 17.0 39.0 11 33.3786357383504 37.0 33.0 39.0 19.0 39.0 12 33.20225160715609 37.0 33.0 39.0 18.0 39.0 13 33.31089688900709 37.0 33.0 39.0 19.0 39.0 14 33.92863438507966 38.0 33.0 40.0 17.0 41.0 15 34.045980609395464 38.0 33.0 40.0 17.0 41.0 16 34.01855039992092 38.0 33.0 40.0 17.0 41.0 17 33.94370864434045 38.0 33.0 40.0 17.0 41.0 18 33.90261746095814 38.0 33.0 40.0 17.0 41.0 19 33.933923908528314 39.0 33.0 40.0 18.0 41.0 20 33.908194113154615 39.0 33.0 40.0 16.0 41.0 21 33.834071356200866 39.0 33.0 40.0 11.0 41.0 22 33.74827281467369 39.0 33.0 40.0 10.0 41.0 23 33.40938440302521 39.0 33.0 40.0 2.0 41.0 24 33.48588656296298 39.0 33.0 40.0 2.0 41.0 25 33.277953572222884 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.574104586223509 1101 2 0.6696178389967002 1101 3 0.653198709053008 1101 4 0.3120715544941888 1101 5 0.45108884346356604 1101 6 0.022973421348645218 1101 7 0.13624600706610934 1101 8 0.4697742228016857 1101 9 0.9339145225382453 1101 10 0.8806429905203643 1101 11 0.5471112585391253 1101 12 0.761466259006859 1101 13 0.6348313774479593 1101 14 0.7439987624042956 1101 15 0.6250061680398531 1101 16 0.6681909590955186 1101 17 0.6811734654616046 1101 18 0.7188519521070091 1101 19 0.6405640533842742 1101 20 0.7450913189012098 1101 21 0.41085292417664476 1101 22 0.6283202143119766 1101 23 0.7822738913146523 1101 24 0.8149244214969329 1101 25 0.8024681918685772 1102 1 1.0913305268104025 1102 2 1.2035279396181942 1102 3 1.0217015314504145 1102 4 0.7289650987488372 1102 5 1.0645135623297222 1102 6 0.6608262992199201 1102 7 0.9370459209174165 1102 8 1.0431773330018608 1102 9 1.4418200503084648 1102 10 1.365606960438022 1102 11 1.176703155914936 1102 12 1.2375325166459632 1102 13 1.0532405667610547 1102 14 1.5043633036379234 1102 15 1.3116438712298972 1102 16 1.265040991110837 1102 17 1.32109114162742 1102 18 1.42720243251555 1102 19 1.539641395518359 1102 20 1.3925117062039334 1102 21 1.4236630739940495 1102 22 1.454981715631689 1102 23 1.7904656132652832 1102 24 1.7683737427855348 1102 25 1.5947068559203927 1103 1 0.5902406542416578 1103 2 0.4017170744270473 1103 3 0.314057519228939 1103 4 0.13194780983013743 1103 5 0.3325849536405556 1103 6 -0.06316580153274742 1103 7 0.3174021322595415 1103 8 0.14283080941590143 1103 9 0.5812585330568112 1103 10 0.6095521128338817 1103 11 0.25511808996367336 1103 12 0.2756221656511002 1103 13 0.268672044516471 1103 14 0.6010266168505325 1103 15 0.5864780615570453 1103 16 0.5343326670926558 1103 17 0.5690511237423337 1103 18 0.5273402967379255 1103 19 0.5229207009807624 1103 20 0.5256904080446034 1103 21 0.6349865705288096 1103 22 0.4565559760087652 1103 23 0.8714701590585605 1103 24 0.5783819401176729 1103 25 0.7779638050224165 1104 1 0.32566435127952786 1104 2 0.5113908652032002 1104 3 0.375044687499571 1104 4 0.13723360743877322 1104 5 -0.014080046567755744 1104 6 0.0520489632883141 1104 7 0.18592898306986072 1104 8 0.39361196177185676 1104 9 0.5832843214999173 1104 10 0.589575443250915 1104 11 0.196875370303637 1104 12 0.31266441037051607 1104 13 0.23125484666620366 1104 14 0.5308635122386107 1104 15 0.5324952260781473 1104 16 0.43310052944319466 1104 17 0.4662983428399414 1104 18 0.4376535909094841 1104 19 0.3091297165204878 1104 20 0.34161445920487665 1104 21 0.5754002688165301 1104 22 0.4330641208239996 1104 23 0.821518069736392 1104 24 0.7175728745841994 1104 25 0.7119668463089184 1105 1 0.9251994904283904 1105 2 0.6629669574843966 1105 3 0.7318247461808411 1105 4 0.7503626391978173 1105 5 0.8043290103551399 1105 6 0.5381663052021111 1105 7 0.5995197817642257 1105 8 0.503006379316421 1105 9 1.0949187340942217 1105 10 1.0096467064451744 1105 11 0.994343773688378 1105 12 0.9426591507136308 1105 13 0.8107285200208878 1105 14 1.1826175046791434 1105 15 1.1532185320303299 1105 16 1.0278839295535462 1105 17 0.9007511580455088 1105 18 1.0058254476199338 1105 19 1.2822384315656805 1105 20 1.395261670297593 1105 21 1.2004666590933795 1105 22 1.0258081147599256 1105 23 1.46796084002861 1105 24 1.064078554497982 1105 25 1.05368469149969 1106 1 1.1012774409589348 1106 2 1.1133950054828148 1106 3 0.8758569239479819 1106 4 0.9116368475392704 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1107 17 0.9668163701917862 1107 18 1.1406374284561096 1107 19 1.1355007622450302 1107 20 1.0561589404868172 1107 21 1.2469122179807783 1107 22 0.934274578069477 1107 23 1.4240460432133872 1107 24 1.237867459471616 1107 25 1.3625781185840964 1108 1 1.4607907047050013 1108 2 1.2615666019066332 1108 3 1.119318705270473 1108 4 0.9642909911617608 1108 5 -0.12017065084915757 1108 6 0.7658016878393639 1108 7 1.064596064424574 1108 8 1.1498727398682398 1108 9 1.4933185079780529 1108 10 1.3647437206266417 1108 11 1.102512346317468 1108 12 1.3370842263906226 1108 13 1.0741702553615085 1108 14 1.590280218098357 1108 15 1.4350574419283362 1108 16 1.4790714810511219 1108 17 1.629113973545067 1108 18 1.6262102236425307 1108 19 1.5726538569277935 1108 20 1.7231894140696227 1108 21 1.603171725130153 1108 22 1.661915030356262 1108 23 2.064484808720607 1108 24 1.5770821266791302 1108 25 1.6726827394812318 1109 1 0.7246475777373504 1109 2 -0.34310311323106646 1109 3 0.6934225096396602 1109 4 0.8437619272427739 1109 5 -0.4662865713574007 1109 6 0.4942942668553627 1109 7 0.7552034651516628 1109 8 0.9953435921573366 1109 9 1.4033173461209998 1109 10 -0.4567384548016804 1109 11 0.4588867303496684 1109 12 0.7789112530861217 1109 13 0.7289721587338747 1109 14 1.200343714234208 1109 15 1.1459796324910343 1109 16 1.276035449229198 1109 17 1.3024002019972585 1109 18 1.3374200710073438 1109 19 1.3977490190673194 1109 20 1.4263457348095443 1109 21 1.5138387390581869 1109 22 1.2955516990831697 1109 23 1.5091732863380116 1109 24 1.3985357955775157 1109 25 1.5970337516142195 1110 1 0.7823924479350062 1110 2 -0.43758459798454297 1110 3 0.19749759469314299 1110 4 0.6115550980835422 1110 5 1.0288734481864168 1110 6 0.5858653629139638 1110 7 0.7300083424528871 1110 8 1.021080947719657 1110 9 1.2625729861541046 1110 10 0.8727831723115855 1110 11 0.5088735689202295 1110 12 0.5805234380456739 1110 13 0.6100748701686314 1110 14 1.1603748869896933 1110 15 0.9871369548432867 1110 16 0.6389771909731081 1110 17 0.857249557718248 1110 18 0.8493717306996516 1110 19 1.0200358264470921 1110 20 0.9400466927092026 1110 21 0.7869185676350696 1110 22 0.8256372844332347 1110 23 1.1430597302459873 1110 24 0.9117968372054932 1110 25 0.9971096552004397 1111 1 0.10706819606919993 1111 2 0.25213534984532515 1111 3 0.3952387004101183 1111 4 0.2944603223426512 1111 5 0.46378758484318183 1111 6 0.03464987128292307 1111 7 0.23630317616058605 1111 8 0.16793805952315566 1111 9 0.4180381698364428 1111 10 0.6448123730941404 1111 11 0.06955395439953804 1111 12 0.12660886663780246 1111 13 -0.04862739778296543 1111 14 0.1635105293344452 1111 15 0.24800029807928325 1111 16 0.027175509935496223 1111 17 0.15998848967969792 1111 18 0.1342007285983584 1111 19 0.06380801686808013 1111 20 -0.1420451846909856 1111 21 0.22112985667209983 1111 22 0.19471198916478016 1111 23 0.4635908511792479 1111 24 0.1657657525014855 1111 25 0.36549419255280213 1112 1 -0.021515602453249016 1112 2 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2203 1 0.20816916079414227 2203 2 0.13024308973780307 2203 3 0.06351508305979792 2203 4 -0.09389485845122181 2203 5 0.02570304253520561 2203 6 -0.12911955209140302 2203 7 0.3094584158689635 2203 8 0.21501626852234423 2203 9 0.6264537379931596 2203 10 0.5704714790665975 2203 11 0.3219620846005853 2203 12 0.3062815229453406 2203 13 0.24920381217358312 2203 14 0.6013023402189006 2203 15 0.49950445891587236 2203 16 0.3849356542760276 2203 17 0.6829356875587749 2203 18 0.541533742811481 2203 19 0.6389497798844985 2203 20 0.502722471248326 2203 21 0.570478115012186 2203 22 0.6413464983333483 2203 23 0.7702886934356741 2203 24 0.6691183557859688 2203 25 0.6860866514967583 2204 1 0.19270495568218138 2204 2 0.25411060779812544 2204 3 -0.024125611061194263 2204 4 -0.12178891897264066 2204 5 -0.0074984303662120055 2204 6 -0.18089066925761443 2204 7 0.10633155989438947 2204 8 0.12755014849455293 2204 9 0.6657023135045534 2204 10 0.5929906372235294 2204 11 0.37205315108631964 2204 12 0.24352753563705676 2204 13 0.23179139617611355 2204 14 0.7346760475607326 2204 15 0.7206403423507695 2204 16 0.6593571301290098 2204 17 0.6254585171708626 2204 18 0.41542015806745525 2204 19 0.6157413240193534 2204 20 0.6185424835584996 2204 21 0.6610973671151683 2204 22 0.6104747796963963 2204 23 1.057057236983816 2204 24 0.8091312778683317 2204 25 0.8013478467353892 2205 1 0.36836052287876697 2205 2 0.23194878396910212 2205 3 0.25067948602331924 2205 4 0.16627092604980476 2205 5 0.15446964621489911 2205 6 -0.049345330815725674 2205 7 -0.11053072670779684 2205 8 0.11113038978444578 2205 9 0.4541147636716616 2205 10 0.566767949360063 2205 11 0.34009345942180147 2205 12 0.28394637656152355 2205 13 0.158450369639624 2205 14 0.48437363381824383 2205 15 0.42382526097321005 2205 16 0.35728924435957765 2205 17 0.3211720870701882 2205 18 0.23291409713930733 2205 19 0.21022684949380732 2205 20 0.29810876235951156 2205 21 0.18630019942865061 2205 22 0.17615550164277494 2205 23 0.4119737168034945 2205 24 0.3622717412488541 2205 25 0.34430662567557846 2206 1 0.1679512297790211 2206 2 0.4904087004657427 2206 3 0.5818039359611902 2206 4 0.5120335459203815 2206 5 0.6686004144145414 2206 6 0.2801524730476004 2206 7 0.5965409896340752 2206 8 0.5344818892511434 2206 9 0.8706220963257181 2206 10 0.7241570590550523 2206 11 0.5257642535589682 2206 12 0.5401932978172113 2206 13 0.5280637492558924 2206 14 0.9420294378114988 2206 15 0.6952962901434674 2206 16 0.7496477084701567 2206 17 0.8431762170077945 2206 18 0.6779099400635218 2206 19 0.6500125730018027 2206 20 0.7583690092840527 2206 21 0.7814133533046004 2206 22 0.6508109084317582 2206 23 0.914333093743771 2206 24 0.9127458781519238 2206 25 0.8193485459403149 2207 1 -0.6600898175943684 2207 2 0.1469660557962129 2207 3 0.3982587191224951 2207 4 0.6081102067142865 2207 5 0.35909095483629727 2207 6 0.7668559016111018 2207 7 0.9004667682307748 2207 8 0.7527055670698921 2207 9 1.1782898159959956 2207 10 0.8955065846146084 2207 11 0.7468731416468799 2207 12 0.7441936460612482 2207 13 0.7291851959995768 2207 14 -4.081677653623167 2207 15 -1.2759281535598106 2207 16 -0.29830818734767206 2207 17 -0.230225466928772 2207 18 0.18165293259856696 2207 19 0.5337234581094492 2207 20 0.6014522644237061 2207 21 0.6392658568133243 2207 22 0.6285443192087357 2207 23 0.8654603235255323 2207 24 0.6001766657007721 2207 25 0.5949675097628955 2208 1 0.47775074302293064 2208 2 1.0333644603117236 2208 3 0.9183418883438179 2208 4 0.7873935097673623 2208 5 1.0118738066582154 2208 6 0.9076215717970015 2208 7 0.9829926622568514 2208 8 1.105502318697578 2208 9 1.4439950589289268 2208 10 1.399570945225591 2208 11 1.0737666416630773 2208 12 1.1036532839364597 2208 13 1.0703602339165244 2208 14 1.3847567755806978 2208 15 1.3557364240357472 2208 16 1.2318373751905867 2208 17 0.9938672837279796 2208 18 1.247298148051705 2208 19 0.9874418201628998 2208 20 0.9493631728190621 2208 21 1.0191897810032131 2208 22 1.1221801352769916 2208 23 1.3907061020233158 2208 24 1.1661767567260455 2208 25 1.1937449008882552 2209 1 -0.3710158285494778 2209 2 -0.27961248710053255 2209 3 0.3807150183121806 2209 4 0.43641242295812077 2209 5 0.734347748967707 2209 6 0.7280202218314926 2209 7 0.9920943633874195 2209 8 0.8608490398103719 2209 9 1.1187316851339233 2209 10 0.7649243167209363 2209 11 0.8581276766366273 2209 12 0.9661258588775681 2209 13 0.7457308218227752 2209 14 1.0531836182649599 2209 15 1.0080506950108727 2209 16 1.1936927697695907 2209 17 1.074728477191961 2209 18 1.0318481284734773 2209 19 0.9336768956478494 2209 20 0.8731274136499252 2209 21 0.7830378042236106 2209 22 0.9331563741438202 2209 23 1.1275548151432133 2209 24 1.217016755980211 2209 25 1.3281982332271411 2210 1 -0.33701671929599186 2210 2 -0.33845428823566337 2210 3 0.23745557126020955 2210 4 0.3657008535312798 2210 5 0.8119546099998303 2210 6 0.6917736906039806 2210 7 0.8148335231165369 2210 8 0.8864431563154476 2210 9 1.0889373562200646 2210 10 1.1610963906757377 2210 11 0.6268624739917996 2210 12 0.5882917432860779 2210 13 0.5469070640144693 2210 14 1.0141300935353286 2210 15 0.6532069753447871 2210 16 1.0249100091405836 2210 17 0.9518008903156243 2210 18 0.81036971765662 2210 19 0.8334610609155817 2210 20 1.1077911568408538 2210 21 0.9614132196198213 2210 22 1.0454087066435065 2210 23 0.8484314010717 2210 24 0.9286409979891843 2210 25 0.9077110634028926 2211 1 -0.9165972048689284 2211 2 -0.0760475851557949 2211 3 0.5497860163210717 2211 4 0.678588458743512 2211 5 0.9163227646510919 2211 6 0.5870156668305384 2211 7 0.7546097058307559 2211 8 0.7626983156470466 2211 9 1.0334372465991564 2211 10 0.9664544697362345 2211 11 0.8300557785377265 2211 12 1.106709008101582 2211 13 0.7742636751081022 2211 14 1.0932048931197187 2211 15 0.5639912890702732 2211 16 0.9113222599913371 2211 17 0.9092551116735947 2211 18 0.8279000063885462 2211 19 0.8225676029003921 2211 20 0.7451523434156329 2211 21 1.0014105505891564 2211 22 1.0750187421988073 2211 23 1.2906402145922513 2211 24 1.1571457688814988 2211 25 1.0506200208604497 2212 1 0.8684752196287526 2212 2 0.010735164913420192 2212 3 0.26452411197992376 2212 4 0.31161901703163863 2212 5 0.41904273307766005 2212 6 0.43635808341522875 2212 7 0.6078983101398983 2212 8 0.5301103351487448 2212 9 0.7992131616493552 2212 10 0.4231921962530123 2212 11 0.38793156222832437 2212 12 0.3741426263419143 2212 13 0.4907910299105822 2212 14 0.38055942070621995 2212 15 0.4887358839859317 2212 16 0.13579844149143128 2212 17 0.7085071080842766 2212 18 0.6915358791444817 2212 19 0.4028864395530576 2212 20 0.5869852712817334 2212 21 0.5398474695094535 2212 22 0.5215579370371017 2212 23 0.8288140216055595 2212 24 0.8917674669743363 2212 25 0.4600825264319397 2213 1 -0.23007383519047764 2213 2 -0.19581284663228615 2213 3 -0.6569586650225396 2213 4 -1.4566871390989284 2213 5 -2.406182270420789 2213 6 -0.9242061772790038 2213 7 -0.768215953358542 2213 8 -0.5197402796257755 2213 9 -1.004730913226755 2213 10 -0.885468117480464 2213 11 -0.8980734896984899 2213 12 -1.097170890965483 2213 13 -1.0422629355361508 2213 14 -7.795873052107957 2213 15 -3.8152422225161757 2213 16 -2.3798264308899704 2213 17 -1.6251280630839133 2213 18 -5.529665333797119 2213 19 -3.219125107241517 2213 20 -5.5309721559709 2213 21 -2.5754582134453834 2213 22 -2.223624682113069 2213 23 -4.902289849995569 2213 24 -2.9097628127329607 2213 25 -2.3442280009929206 2214 1 -0.1235624345614319 2214 2 -0.19133311862314528 2214 3 -2.198680218508798 2214 4 -2.813422560540232 2214 5 -0.8339601629045639 2214 6 -0.9250348134017585 2214 7 -0.8702283553709442 2214 8 -1.0615664449813522 2214 9 -6.199304172505897 2214 10 -2.483340780059013 2214 11 -1.428644243798658 2214 12 -1.7254181603892214 2214 13 -1.9436724428280137 2214 14 -3.2074354166114993 2214 15 -2.704026728947742 2214 16 -2.131157823397835 2214 17 -2.5557772760860686 2214 18 -1.8889974695998433 2214 19 -5.250606397546338 2214 20 -3.0015046441504474 2214 21 -4.843284557742315 2214 22 -6.647630353177561 2214 23 -7.169742482154081 2214 24 -6.704729742994012 2214 25 -4.648244546185936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34122.0 3 20.0 4 625.0 5 4057.0 6 4975.0 7 4021.0 8 2901.0 9 2497.0 10 2048.0 11 1921.0 12 1863.0 13 1788.0 14 1842.0 15 1865.0 16 1899.0 17 1947.0 18 2251.0 19 2614.0 20 3255.0 21 4270.0 22 5498.0 23 6754.0 24 8147.0 25 9411.0 26 10907.0 27 11926.0 28 13756.0 29 16517.0 30 20276.0 31 25198.0 32 32103.0 33 41115.0 34 53203.0 35 69588.0 36 93217.0 37 137345.0 38 214051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.35903065022189 20.26172349787531 9.608109636022725 29.771136215880073 2 13.323704491066247 21.865123226815737 39.96828838500302 24.842883897114994 3 18.18823886704013 25.465347187317178 29.192009200413878 27.154404745228817 4 11.814914697985076 18.222718717003076 38.13134850519887 31.831018079812974 5 12.690194010543873 39.03492613522004 35.50205804426355 12.77282180997254 6 30.831749391920066 39.08592503162961 17.82445007011082 12.257875506339506 7 27.42197463866343 32.364347024557446 22.08154855500732 18.132129781771805 8 24.295513566336954 39.063293854980415 19.98766964551999 16.65352293316264 9 25.888826933527927 16.140288418578276 20.17928021492878 37.79160443296502 10 14.437505737575648 30.598167271220788 34.14957947984022 20.814747511363343 11 33.54652927982527 23.507177269977852 24.617835968960865 18.32845748123601 12 22.9021674406125 25.808171267584417 31.874015896290476 19.415645395512605 13 28.636032539689076 23.035021469934446 25.753212841244867 22.57573314913161 14 20.135612061889784 23.646852813337933 27.74171060084528 28.475824523927002 15 22.14284201285512 32.47083355888244 23.34209640635554 22.044228021906896 16 21.11371312237429 29.53681584428728 28.346376078232932 21.0030949551055 17 20.752050292024663 29.907255839022213 28.15593163692679 21.184762232026333 18 20.594828874607682 28.985227541520693 30.26229851592867 20.15764506794295 19 22.725635932292747 27.91829846924366 27.750018828491275 21.606046769972323 20 22.7527124667561 28.520558262138433 28.500082374261577 20.22664689684389 21 21.89726650435225 27.701870224561098 27.63679540028077 22.764067870805878 22 22.05231156293356 29.310432069927618 27.682153183186962 20.955103183951856 23 21.46475671134029 28.930692533358123 28.15085556129552 21.45369519400607 24 22.384079775284864 27.82814987755755 29.01703603373551 20.77073431342207 25 21.78754120121018 28.51800379621861 28.419509221657506 21.274945780913704 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 328.0 1 328.0 2 328.5 3 329.0 4 329.0 5 329.0 6 542.5 7 756.0 8 756.0 9 756.0 10 1341.0 11 1926.0 12 1926.0 13 1926.0 14 3191.5 15 4457.0 16 4457.0 17 4457.0 18 7683.5 19 10910.0 20 10910.0 21 10910.0 22 16987.5 23 23065.0 24 23065.0 25 23065.0 26 33068.0 27 43071.0 28 43071.0 29 43071.0 30 56777.5 31 70484.0 32 70484.0 33 70484.0 34 79972.5 35 89461.0 36 89461.0 37 89461.0 38 95984.5 39 102508.0 40 102508.0 41 102508.0 42 106215.0 43 109922.0 44 109922.0 45 109922.0 46 111199.5 47 112477.0 48 112477.0 49 112477.0 50 106348.5 51 100220.0 52 100220.0 53 100220.0 54 86928.0 55 73636.0 56 73636.0 57 73636.0 58 61802.5 59 49969.0 60 49969.0 61 49969.0 62 39960.5 63 29952.0 64 29952.0 65 29952.0 66 22798.5 67 15645.0 68 15645.0 69 15645.0 70 11220.5 71 6796.0 72 6796.0 73 6796.0 74 4719.5 75 2643.0 76 2643.0 77 2643.0 78 1765.5 79 888.0 80 888.0 81 888.0 82 576.0 83 264.0 84 264.0 85 264.0 86 166.0 87 68.0 88 68.0 89 68.0 90 41.5 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08578559719837654 2 0.030831037676234095 3 0.03130174054152011 4 0.03318455200266418 5 0.023535143264300836 6 0.3855056466692477 7 0.16651113859492842 8 0.1741600601558262 9 0.17792568307811432 10 0.015415518838117048 11 0.0031772443406806123 12 0.004118650071252646 13 0.0 14 0.07131148409083153 15 0.001765135744822563 16 0.002706541475394596 17 0.004236325787574151 18 0.004236325787574151 19 0.002000487177465571 20 0.0015297843121795543 21 2.3535143264300835E-4 22 0.0 23 0.0 24 7.060542979290251E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 849793.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.6551411676253 #Duplication Level Percentage of deduplicated Percentage of total 1 92.93817524518838 78.6769434524292 2 5.386670129840591 9.120186405301716 3 0.8369931049105053 2.125673083575835 4 0.28597013481074407 0.9683536852851351 5 0.1543063775346525 0.6531414086630447 6 0.08795083224491633 0.4467294071702114 7 0.06135845272188634 0.36360159338988457 8 0.034604692873819176 0.23435721282363833 9 0.028179448601779244 0.2146981679468517 >10 0.15688697591395032 2.443470030845499 >50 0.015640974505323545 0.9225615617077899 >100 0.011029756282957054 1.9087004987417677 >500 0.0011169372852543264 0.624768750113378 >1k 0.0011169372852543264 1.2968147420060505 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1853 0.21805310234374722 No Hit CTGTAGGACGTGGAATATGGCAAGA 1654 0.19463563479576793 No Hit GTCCTACAGTGGACATTTCTAAATT 1550 0.18239736029833148 No Hit CTTTAGGACGTGAAATATGGCGAGG 1286 0.15133097118945438 No Hit GTCCTACAGTGTGCATTTCTCATTT 1237 0.14556486108970068 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1236 0.14544718537337917 No Hit GGTATCAACGCAGAGTACTTTTTTT 1131 0.13309123515962124 No Hit CTGTAGGACCTGGAATATGGCGAGA 1025 0.12061760922954179 No Hit TATCAACGCAGAGTACTTTTTTTTT 872 0.10261322463235165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1767571632150418E-4 0.0 4 0.0 0.0 0.0 1.1767571632150418E-4 0.0 5 0.0 0.0 0.0 1.1767571632150418E-4 0.0 6 0.0 0.0 0.0 1.1767571632150418E-4 0.0 7 0.0 0.0 0.0 1.1767571632150418E-4 0.0 8 0.0 0.0 0.0 1.1767571632150418E-4 0.0 9 0.0 0.0 0.0 1.1767571632150418E-4 0.0 10 0.0 0.0 0.0 1.1767571632150418E-4 0.0 11 0.0 0.0 0.0 1.1767571632150418E-4 0.0 12 0.0 0.0 0.0 1.1767571632150418E-4 0.0 13 0.0 0.0 0.0 1.1767571632150418E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAC 40 0.0053431476 14.22306 13 TAGGACC 325 0.0 13.2044 4 TGTAGGA 1040 0.0 12.105468 2 CTAGACC 95 9.909018E-7 12.046121 4 TAGACAG 175 0.0 11.988758 5 GTAGGAC 1035 0.0 11.978227 3 TCTAGAC 105 2.582201E-7 11.80711 3 TCTACAC 130 2.4538167E-9 11.737246 3 CTAGACT 115 6.689879E-8 11.609666 4 TCCAACG 115 7.320705E-8 11.534523 18 GGCGAGG 360 0.0 11.317106 19 TAGGACT 170 1.0913936E-11 11.219425 4 CTAATAC 60 0.00570659 11.125931 3 GCACCGT 60 0.005709107 11.125272 6 GTCCTAA 435 0.0 10.956965 1 CTGTAGG 1105 0.0 10.955974 1 AGGACGT 985 0.0 10.940363 5 GACAGAT 105 3.4646964E-6 10.855795 7 ATGCCGG 70 0.001523599 10.828328 19 TAGGACG 1025 0.0 10.79254 4 >>END_MODULE