FastQCFastQC Report
Thu 26 May 2016
SRR1926639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895019
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2600.014.0453734
TGTAGGA5150.013.8527982
GTCATAA802.554202E-413.3898731
GTCTTAT904.6021298E-513.3898731
CTGTAGG5200.013.3898721
CCTAATG500.00891639613.1633443
TTTAGAC600.002001155212.7976973
ATTAAGC600.002001155212.7976973
GTCCTAC5450.012.7756581
GGACCTG4250.012.7666696
GTCCTAA2650.012.6319561
AGGACCT4400.012.5753695
GACAGTT654.412277E-412.5519917
CTACAAC704.4576067E-412.5365193
GATATAC1502.3914254E-812.4972141
GTAGGAC5300.012.418253
AGGCGTG751.06938656E-412.2381927
GTATAAT1102.2174969E-512.1726111
GTATTAA1001.2337879E-412.0508851
CCAACGA500.003428791411.91170219
Produced by FastQC (version 0.11.5)