FastQCFastQC Report
Thu 26 May 2016
SRR1926639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895019
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19420.21697863397313355No Hit
CTGTAGGACGTGGAATATGGCAAGA15820.1767560241737885No Hit
GTCCTACAGTGGACATTTCTAAATT14760.16491270017731469No Hit
CTTTAGGACGTGAAATATGGCGAGG13340.14904711520090635No Hit
GTCCTAAAGTGTGTATTTCTCATTT12430.13887973327940525No Hit
GGTATCAACGCAGAGTACTTTTTTT11800.13184077656451987No Hit
GTCCTACAGTGTGCATTTCTCATTT10300.11508135581479274No Hit
TATCAACGCAGAGTACTTTTTTTTT9800.1094948822315504No Hit
CTGTAGGACCTGGAATATGGCGAGA9250.10334976128998379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC400.005275120514.2506728
TAGGACC3500.013.0819664
TTGTAAC550.002969139712.1431933
GACGTGG4050.011.9635287
GCCGGTT658.158678E-411.66801511
TCCAACG658.21241E-411.65888718
GGCGAGG4450.011.4951419
CGTCAAG752.0019883E-411.4422141
ACGTGGA4450.011.3151418
AGGACGT9400.010.9596265
TAGGACG9950.010.92902854
ATAGGAC1407.781637E-910.9040923
GGACGTG9200.010.8862136
CGTGGAA5000.010.8305119
TTAGGAC6450.010.7984323
TGTAGGA10200.010.7583122
CTAATAC803.6199787E-410.7337163
TAGGTTG803.6275192E-410.73135
CTGTAGG9800.010.60545351
GACAGTC909.499195E-510.5560547
Produced by FastQC (version 0.11.5)