FastQCFastQC Report
Thu 26 May 2016
SRR1926638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838468
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9360.11163216723834421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGA702.108718E-719.0479681
GTCTTAG901.6325976E-716.2965951
CTCCTAG758.558287E-616.0002941
AGTACTA400.002865126415.6194575
GTCCTAA3300.015.3538171
GTATAGA801.5774345E-515.0002741
TCTAGTA550.00390892914.9063592
TAGGACC2850.014.6557014
AGAGTAG601.8798102E-413.8839635
TAAAGGT703.5394944E-513.7699174
TATAGGA1101.1998691E-613.6641622
CTTATAC652.464309E-413.4473053
ATATTAC652.464309E-413.4473053
TGTAGGA6250.013.3362232
GTCCAAA600.00818715913.3335771
GTGTAGG957.9459365E-512.6318111
GCCTTAG750.002467577312.4446731
TCCAACG901.4767829E-712.32511918
CTGTAGG6750.012.2471391
TGTTATA905.8143405E-412.1459232
Produced by FastQC (version 0.11.5)