##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926638_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 838468 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.331836158326855 27.0 7.0 31.0 2.0 31.0 2 16.494146467128143 18.0 2.0 31.0 2.0 31.0 3 20.02062571260919 24.0 15.0 31.0 2.0 31.0 4 22.177186249206887 28.0 2.0 35.0 2.0 37.0 5 22.858631456418134 30.0 2.0 35.0 2.0 37.0 6 23.60008133882271 31.0 2.0 35.0 2.0 37.0 7 23.356561013658244 30.0 7.0 35.0 2.0 37.0 8 23.389028561614754 30.0 8.0 35.0 2.0 37.0 9 26.15755043722599 31.0 17.0 37.0 2.0 39.0 10 26.898025923469948 32.0 23.0 37.0 2.0 39.0 11 24.027427403311755 30.0 7.0 37.0 2.0 39.0 12 25.170874738213026 31.0 11.0 37.0 2.0 39.0 13 21.845082936975533 27.0 2.0 37.0 2.0 39.0 14 25.170822261553212 31.0 17.0 38.0 2.0 40.0 15 26.26136835275765 31.0 17.0 38.0 2.0 40.0 16 26.64016158040617 31.0 17.0 39.0 2.0 40.0 17 25.965805492875102 31.0 11.0 38.0 2.0 40.0 18 26.044371401174523 31.0 16.0 38.0 2.0 40.0 19 26.421652346899346 31.0 17.0 39.0 2.0 40.0 20 26.673559396423 31.0 17.0 39.0 2.0 40.0 21 24.528573541268123 30.0 2.0 38.0 2.0 40.0 22 25.45934012985588 31.0 2.0 38.0 2.0 40.0 23 25.460091500212293 31.0 2.0 38.0 2.0 40.0 24 24.5162618012852 31.0 2.0 39.0 2.0 40.0 25 24.32779664817262 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.90278432520882 1101 2 10.543017570570568 1101 3 7.137126395178214 1101 4 8.663331087227466 1101 5 8.553315757988614 1101 6 8.045412239545502 1101 7 7.415682518753147 1101 8 6.250261486474269 1101 9 6.067073862664497 1101 10 5.509298560638577 1101 11 8.126745786263943 1101 12 5.672806559883849 1101 13 10.132133010021064 1101 14 7.5506360492149405 1101 15 6.799824550063402 1101 16 6.274814069675202 1101 17 2.1217637367494255 1101 18 4.795723433149131 1101 19 5.535309597508164 1101 20 3.6417547891541666 1101 21 0.542875536262553 1101 22 4.470133124592362 1101 23 5.7262811031574365 1101 24 7.035758929267256 1101 25 7.65943567695513 1102 1 5.649947484999288 1102 2 10.776846186945063 1102 3 6.620421109858693 1102 4 8.175125138725935 1102 5 8.043804319398891 1102 6 6.992311355523412 1102 7 6.897731699158506 1102 8 6.960104941487085 1102 9 6.0130569082723255 1102 10 5.343556000046995 1102 11 8.129005710123273 1102 12 5.52468346422776 1102 13 9.876443967224791 1102 14 7.283766977319964 1102 15 5.437724810892934 1102 16 5.7241120799401095 1102 17 6.759528335970998 1102 18 6.649731690072365 1102 19 5.491645939976674 1102 20 5.643642707140813 1102 21 4.886209540813596 1102 22 5.850493294344265 1102 23 6.075182433208422 1102 24 7.411292003756351 1102 25 7.31555902597227 1103 1 5.276820689440626 1103 2 9.249372521805432 1103 3 6.402987044066833 1103 4 8.209211636124802 1103 5 7.590288259663403 1103 6 7.169343733813655 1103 7 7.543110481155036 1103 8 6.857625452913954 1103 9 5.954279052848765 1103 10 5.117270315340626 1103 11 6.140432580739521 1103 12 4.945769469870022 1103 13 9.155282903995243 1103 14 7.336190819750794 1103 15 6.245849147668562 1103 16 6.190504398184281 1103 17 3.9762894716699435 1103 18 2.511418674051452 1103 19 4.68587685852625 1103 20 5.044165522059156 1103 21 7.469238761508787 1103 22 3.0339443904438603 1103 23 5.169431469106652 1103 24 6.837541453765411 1103 25 2.5250085173924113 1104 1 5.872133214764219 1104 2 10.468941546414483 1104 3 7.116434074973775 1104 4 7.760966626719917 1104 5 7.805409388669059 1104 6 7.5189733062417545 1104 7 7.316424632908788 1104 8 7.025770779119977 1104 9 6.014418357640444 1104 10 4.80039290870544 1104 11 8.187496569230575 1104 12 5.024643228954339 1104 13 9.358704890165821 1104 14 7.101559202368012 1104 15 6.321348493187731 1104 16 6.06590230632434 1104 17 6.482698426378725 1104 18 4.018015440448988 1104 19 4.988347583260097 1104 20 4.056224557851106 1104 21 3.2908500713952122 1104 22 5.426231050189141 1104 23 5.517985652778858 1104 24 6.410920529255222 1104 25 5.6674670200661055 1105 1 3.777939509096697 1105 2 9.652461703186775 1105 3 6.512047826304041 1105 4 5.958155133210685 1105 5 2.1548404796523073 1105 6 4.895811419160935 1105 7 6.039277100994095 1105 8 3.1904487255154805 1105 9 4.1064428486227555 1105 10 3.83452088627725 1105 11 6.810514060780154 1105 12 4.290592137314746 1105 13 5.376789024003603 1105 14 3.682852011189265 1105 15 4.366661498630691 1105 16 4.7155903088372 1105 17 5.219794482224167 1105 18 2.600100080608758 1105 19 2.581294236612372 1105 20 1.6317410766061187 1105 21 2.7411400344993417 1105 22 4.106584819240677 1105 23 1.6376009418277704 1105 24 4.669250229259443 1105 25 5.304159424767398 1106 1 6.713491911116812 1106 2 11.311148070174822 1106 3 7.943946764768363 1106 4 9.30976134938554 1106 5 8.67507226450699 1106 6 7.804901948815761 1106 7 7.589642308223109 1106 8 7.23932110471884 1106 9 6.3281632877061895 1106 10 5.6560855036096385 1106 11 7.996241066637381 1106 12 5.170987813261153 1106 13 4.209766193334541 1106 14 5.370575483231782 1106 15 5.600462297217167 1106 16 5.762029364446086 1106 17 6.083753745966391 1106 18 6.003113561972757 1106 19 5.98793057368821 1106 20 5.3855691439990245 1106 21 5.632234543173883 1106 22 5.97201465075841 1106 23 6.582907507210297 1106 24 7.1517550639075225 1106 25 6.1979418586475 1107 1 4.104269839838437 1107 2 -13.896679190284017 1107 3 -5.4406847603772395 1107 4 2.3118901059818597 1107 5 4.895807017310286 1107 6 5.163994223330313 1107 7 5.07877659295103 1107 8 5.723768358811139 1107 9 3.897177056475158 1107 10 3.340979312371257 1107 11 5.966226405655771 1107 12 3.195632609509378 1107 13 4.9953594250722055 1107 14 4.366285240630646 1107 15 4.218159940251407 1107 16 3.9076694037213784 1107 17 4.620148399864107 1107 18 -0.5391813524151807 1107 19 2.1304944969130872 1107 20 2.6469412684066462 1107 21 -0.4312703729626115 1107 22 2.5555167871369235 1107 23 -0.3055461851601642 1107 24 3.1768571751785437 1107 25 3.962416551641166 1108 1 5.125223787278429 1108 2 10.40325532183124 1108 3 -0.029407355002881275 1108 4 5.5496511600539655 1108 5 7.140840925239978 1108 6 7.160857767029878 1108 7 7.1715909837505265 1108 8 6.620579903824812 1108 9 5.425525478130112 1108 10 4.393235163311257 1108 11 6.825353480435972 1108 12 2.5586495776161264 1108 13 3.298809831597495 1108 14 4.702640154506465 1108 15 4.561584499731158 1108 16 4.662672047169217 1108 17 0.14668839860221183 1108 18 3.412614342789844 1108 19 4.437238443635103 1108 20 4.904473136638931 1108 21 4.710538963738667 1108 22 1.6592012965325829 1108 23 4.670902543917979 1108 24 5.173196380664731 1108 25 5.653795865737159 1109 1 3.1932545383451263 1109 2 -13.896679190284017 1109 3 -5.8876670985109065 1109 4 1.8039351031507742 1109 5 1.4102946594613641 1109 6 2.2129871990603114 1109 7 3.100367866392741 1109 8 3.6376202010618144 1109 9 3.0028744693880824 1109 10 1.9483787861763453 1109 11 0.2654912335963999 1109 12 1.0815642860336716 1109 13 2.1926643247790913 1109 14 2.7193119983467717 1109 15 2.937654392920013 1109 16 2.6230848225511494 1109 17 3.2368129062664472 1109 18 3.395258036252752 1109 19 1.7514417841895842 1109 20 1.133934885394055 1109 21 -0.09252000628616841 1109 22 2.2265280864563266 1109 23 2.6704466836936014 1109 24 3.691696371924049 1109 25 4.0175346726205134 1110 1 3.098012993595475 1110 2 8.945925965753974 1110 3 6.0426124353114865 1110 4 7.477490975088454 1110 5 8.137444796400338 1110 6 3.6715330919590805 1110 7 6.16951438691687 1110 8 6.845812021500411 1110 9 4.809523509050699 1110 10 4.5480646948818375 1110 11 2.5635343389581493 1110 12 5.086340201119434 1110 13 9.710222139587817 1110 14 7.290393031486094 1110 15 6.414512216923018 1110 16 5.9385821910381935 1110 17 6.387299506307123 1110 18 6.606182135190579 1110 19 5.558355043170021 1110 20 5.5294568090964304 1110 21 3.398696674914884 1110 22 5.410237595658494 1110 23 5.98515464472845 1110 24 6.832534091112315 1110 25 7.263180626164349 1111 1 4.890015174703528 1111 2 9.4130671381539 1111 3 7.446706179480557 1111 4 8.065791431780596 1111 5 8.469415239125421 1111 6 7.574041728531046 1111 7 7.461162409698943 1111 8 7.600512544857651 1111 9 5.9336039202004365 1111 10 5.238492275391717 1111 11 7.186240688265141 1111 12 6.458398467863152 1111 13 8.135437668932234 1111 14 6.067756970378838 1111 15 5.728086353763995 1111 16 5.314447433342266 1111 17 6.57050080772575 1111 18 6.370372471287741 1111 19 5.135052512251367 1111 20 4.823365648199857 1111 21 7.609183565622168 1111 22 6.813882980821525 1111 23 7.006255597061092 1111 24 6.688513026391014 1111 25 7.225095472372683 1112 1 -2.554250823895547 1112 2 7.0384997428692575 1112 3 5.303453280499333 1112 4 -0.6774741782743128 1112 5 3.4379467675463893 1112 6 2.400902480972519 1112 7 4.869413699512325 1112 8 5.172471645851186 1112 9 3.654254186276564 1112 10 2.6777581342591326 1112 11 -1.0602080991795653 1112 12 3.2471381694143524 1112 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6 3.922967788497882 2204 7 5.216222935462547 2204 8 5.059781370607698 2204 9 3.7046495981651226 2204 10 3.1708805687304498 2204 11 4.824854807043817 2204 12 4.97715040600599 2204 13 3.260319987684543 2204 14 4.084039227110576 2204 15 4.3049148000372846 2204 16 4.384292066241766 2204 17 -0.6427097595396418 2204 18 2.734738682332228 2204 19 3.6109703331381873 2204 20 3.906429575810339 2204 21 5.1209052678083005 2204 22 4.802107428439211 2204 23 3.78667492296805 2204 24 5.189359774303391 2204 25 5.4628171634173945 2205 1 6.076172700113393 2205 2 10.586149092544266 2205 3 7.315470367870912 2205 4 8.190762998808239 2205 5 8.055913061137261 2205 6 7.76043705698245 2205 7 7.599053559739627 2205 8 7.4494302937754 2205 9 6.13501725245489 2205 10 5.37440772719502 2205 11 7.874896718977247 2205 12 7.149212707275524 2205 13 10.05690287731333 2205 14 7.647390870573485 2205 15 6.512185466835074 2205 16 6.065925273605153 2205 17 6.791726864465829 2205 18 6.5884023472615425 2205 19 5.078215337657184 2205 20 5.399771900307044 2205 21 -0.6069317486239889 2205 22 3.90603637951698 2205 23 5.341262991881571 2205 24 7.1648522980573865 2205 25 7.5473959133647135 2206 1 4.114380769507903 2206 2 8.575185216495646 2206 3 5.892424085841579 2206 4 2.081510017012885 2206 5 5.758950212310005 2206 6 6.044723994150743 2206 7 6.152618588558891 2206 8 6.125403586019878 2206 9 4.592288267692009 2206 10 3.8477330710288378 2206 11 3.724913268654813 2206 12 4.635022214779944 2206 13 8.092029282609229 2206 14 0.6287684885612741 2206 15 2.5682900151430097 2206 16 3.4510693699356914 2206 17 4.463328759118568 2206 18 -0.6373557991745713 2206 19 1.3057813936978135 2206 20 2.689605832852841 2206 21 3.7759935495555474 2206 22 -1.9575120996247186 2206 23 -2.502991987288663 2206 24 1.4346599793904922 2206 25 2.9268514860406256 2207 1 1.669744024119499 2207 2 -13.896679190284017 2207 3 -6.127715110097174 2207 4 -4.455015699144372 2207 5 1.294786265227856 2207 6 2.0128619267986245 2207 7 3.2571835135217526 2207 8 3.1695256573490234 2207 9 0.9582809452837999 2207 10 0.7493957021829942 2207 11 -1.1057879747509247 2207 12 -1.6608297462451915 2207 13 0.9302784550367349 2207 14 -4.832170791596873 2207 15 -1.203539066280765 2207 16 -0.1787157536445676 2207 17 1.1053266302395066 2207 18 0.9163693882720594 2207 19 0.5056658259772355 2207 20 0.25789348886341656 2207 21 -3.367226204570617 2207 22 -3.96742285046399 2207 23 -1.116013708873389 2207 24 0.6341958369661427 2207 25 1.2308390402860994 2208 1 4.46814065098545 2208 2 6.053744814934509 2208 3 6.136453849128365 2208 4 7.744781988443666 2208 5 3.1756110191913827 2208 6 5.717807236270435 2208 7 6.245602546014638 2208 8 6.349044834485831 2208 9 4.543445962253465 2208 10 4.062507248171254 2208 11 5.947749183610533 2208 12 5.394267297535592 2208 13 8.148426067466659 2208 14 2.6218504039371275 2208 15 4.2289368027644905 2208 16 4.550582676070775 2208 17 5.455431095293346 2208 18 0.05765719185885132 2208 19 2.9065469248928792 2208 20 3.8301539991822935 2208 21 6.626524799901052 2208 22 5.691113504320132 2208 23 1.432016581265298 2208 24 3.0667189752665287 2208 25 4.375350607072832 2209 1 2.37317740286365 2209 2 4.729175802876037 2209 3 4.858846214803531 2209 4 0.2639909463179926 2209 5 4.754650089422853 2209 6 4.869563893058668 2209 7 5.320031234342469 2209 8 3.897952451052685 2209 9 3.7226998237215945 2209 10 2.6936664337314298 2209 11 -1.4791517456047352 2209 12 2.396414317541428 2209 13 1.5734325147732022 2209 14 2.966695637347474 2209 15 3.3899793656960036 2209 16 2.9789220908427367 2209 17 0.7424857068799362 2209 18 2.7475100683391673 2209 19 3.24272822761467 2209 20 3.407953580308149 2209 21 5.5798494237932985 2209 22 4.044416533081812 2209 23 -0.3457650634091358 2209 24 -3.7301391117083043 2209 25 -0.44200009873991775 2210 1 -2.230684905478391 2210 2 -13.896679190284017 2210 3 -7.61248854616395 2210 4 -2.1197386420731483 2210 5 -0.3945280589241129 2210 6 -1.1735739390538633 2210 7 -1.0717600617069074 2210 8 -20.756630312285207 2210 9 -13.022443430322248 2210 10 -7.264568533300228 2210 11 -9.906060532866082 2210 12 -4.8503056833821105 2210 13 -5.4041293862505775 2210 14 -4.202150961246826 2210 15 -6.070549051342486 2210 16 -5.232139764966213 2210 17 -4.712168630568911 2210 18 -4.792299182807078 2210 19 -10.231097196971808 2210 20 -7.107976469466642 2210 21 -5.229060206195484 2210 22 -9.715359008967017 2210 23 -7.491746427203797 2210 24 -10.503651303357607 2210 25 -8.087420606261935 2211 1 -0.6281763264038069 2211 2 4.1651693156228635 2211 3 0.015839357746504135 2211 4 0.7690246517050561 2211 5 0.06849615405391773 2211 6 -1.1116360016090248 2211 7 -20.751788191102126 2211 8 -13.573169575662552 2211 9 -9.851512394255437 2211 10 -8.117244491072903 2211 11 -10.05716562323103 2211 12 -5.7855909699881956 2211 13 -7.086020917035064 2211 14 -5.691283807642542 2211 15 -6.315007472519092 2211 16 -12.294323028269277 2211 17 -8.712082131212522 2211 18 -7.029227273008804 2211 19 -9.504803640636293 2211 20 -7.766369008432889 2211 21 -5.510233938931044 2211 22 -7.005274552363794 2211 23 -9.034602215672308 2211 24 -22.03178069857561 2211 25 -21.822301056332257 2212 1 -1.4325796998132212 2212 2 -13.896679190284017 2212 3 -7.9287947442376385 2212 4 -19.594422186376946 2212 5 -20.142066736842537 2212 6 -20.871886175340784 2212 7 -10.141657722951704 2212 8 -9.534066996859266 2212 9 -5.407545752886318 2212 10 -5.570013719298569 2212 11 -5.642759247182173 2212 12 -3.0850495882300564 2212 13 -6.48264558763805 2212 14 -3.8467901310518755 2212 15 -4.03031138709693 2212 16 -4.086804376438387 2212 17 -4.605392965194294 2212 18 -3.502524315115622 2212 19 -4.079105633631556 2212 20 -4.2154300036132 2212 21 -2.052342623812301 2212 22 -3.441130867266537 2212 23 -3.6097922006164467 2212 24 -2.72863411377131 2212 25 -3.0878421154266533 2213 1 -18.759517535576844 2213 2 -13.896679190284017 2213 3 -8.685203032802487 2213 4 -6.078713374116409 2213 5 -20.142066736842537 2213 6 -10.939319125532354 2213 7 -20.751788191102126 2213 8 -10.640155216499766 2213 9 -8.628549449042847 2213 10 -7.339931063005839 2213 11 -6.178023285666896 2213 12 -5.416118768400782 2213 13 -19.12424190383145 2213 14 -12.390482112127083 2213 15 -7.235266059926801 2213 16 -6.4125607359153385 2213 17 -5.100203937809805 2213 18 -5.059014348431017 2213 19 -5.919578694619609 2213 20 -5.902074146366587 2213 21 -4.069256141982866 2213 22 -4.898723866622575 2213 23 -4.668675473229307 2213 24 -3.713006752215456 2213 25 -4.784753163611953 2214 1 -18.759517535576844 2214 2 -13.896679190284017 2214 3 -11.994093803335934 2214 4 -19.594422186376946 2214 5 -20.142066736842537 2214 6 -20.871886175340784 2214 7 -16.341966006512795 2214 8 -14.534784418974454 2214 9 -13.288156055561771 2214 10 -13.217541114880833 2214 11 -14.288234451605238 2214 12 -11.726233256739452 2214 13 -19.12424190383145 2214 14 -16.41190431099096 2214 15 -23.763235408585803 2214 16 -17.976021145140585 2214 17 -23.469552596813635 2214 18 -17.519129647823377 2214 19 -14.458565396583332 2214 20 -13.07884491988471 2214 21 -10.618472501909027 2214 22 -11.644610364525192 2214 23 -11.6272325762753 2214 24 -10.468698564790682 2214 25 -10.239743901378828 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 151130.0 3 7.0 4 721.0 5 2875.0 6 4240.0 7 3954.0 8 3236.0 9 3027.0 10 2796.0 11 2600.0 12 2548.0 13 2710.0 14 4084.0 15 8661.0 16 12190.0 17 12991.0 18 13404.0 19 15697.0 20 18432.0 21 21542.0 22 22789.0 23 24360.0 24 26077.0 25 26671.0 26 22622.0 27 17564.0 28 18808.0 29 21236.0 30 21061.0 31 23881.0 32 30964.0 33 38652.0 34 47434.0 35 54559.0 36 54170.0 37 57201.0 38 43574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09846265266485 19.53931702713676 13.038373330699763 25.323846989498634 2 13.340384026070124 20.354577593745564 42.93311309261322 23.3719252875711 3 18.456205549762807 24.908669516194593 30.083895286160434 26.551229647882167 4 11.368118788393692 17.878530426699253 39.09915675246258 31.654194032444483 5 12.188243055651004 37.52023860247124 37.30417691768489 12.987341424192861 6 30.758363109433795 38.09750940701628 18.58698107997289 12.557146403577038 7 26.522238526406195 31.597991511872053 23.63134864287427 18.24842131884749 8 23.653913065890027 38.29611206329979 21.584556502016778 16.465418368793415 9 25.728197070496257 16.005052844732482 20.92369631352472 37.343053771246545 10 14.136548710889398 30.128197374119082 35.11891945508253 20.616334459908987 11 32.50135415469813 22.856111120229997 25.66340954057224 18.979125184499633 12 22.966014548389012 25.230868084234437 32.439416496016335 19.363700871360216 13 27.75554401292018 22.264750210747707 25.998177777399327 23.98152799893278 14 19.674784730560326 23.313313810562892 28.451186814012292 28.560714644864486 15 21.639957645544307 32.51404649654457 24.018710645856313 21.827285212054807 16 20.70779924694894 28.983135535686444 29.04599209627857 21.263073121086045 17 20.283834429506793 29.161120307288773 29.70351741515912 20.851527848045308 18 20.208771602801374 28.198449222069172 31.674142968846454 19.918636206283 19 22.235636682122866 27.430782798253645 27.968745652565758 22.364834867057727 20 22.39279472404219 28.57395779377302 28.9351068194841 20.098140662700686 21 21.240714465609546 27.01601139075424 27.558618247649157 24.184655895987053 22 21.644012537209996 29.054269351319327 28.791603413203326 20.51011469826735 23 21.037103370048655 28.491567415898032 29.16736813826453 21.303961075788784 24 21.767136590780716 27.768596557675902 29.491331282739498 20.972935568803887 25 21.631657878648937 28.00249312242091 29.295860079474245 21.069988919455906 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1022.0 1 1022.0 2 1974.0 3 2926.0 4 2926.0 5 2926.0 6 4215.5 7 5505.0 8 5505.0 9 5505.0 10 6679.0 11 7853.0 12 7853.0 13 7853.0 14 9846.5 15 11840.0 16 11840.0 17 11840.0 18 16929.0 19 22018.0 20 22018.0 21 22018.0 22 30283.0 23 38548.0 24 38548.0 25 38548.0 26 49387.0 27 60226.0 28 60226.0 29 60226.0 30 71548.0 31 82870.0 32 82870.0 33 82870.0 34 89722.5 35 96575.0 36 96575.0 37 96575.0 38 99761.5 39 102948.0 40 102948.0 41 102948.0 42 102553.5 43 102159.0 44 102159.0 45 102159.0 46 98591.5 47 95024.0 48 95024.0 49 95024.0 50 87122.0 51 79220.0 52 79220.0 53 79220.0 54 67532.0 55 55844.0 56 55844.0 57 55844.0 58 45853.5 59 35863.0 60 35863.0 61 35863.0 62 28356.0 63 20849.0 64 20849.0 65 20849.0 66 15639.5 67 10430.0 68 10430.0 69 10430.0 70 7400.5 71 4371.0 72 4371.0 73 4371.0 74 3010.5 75 1650.0 76 1650.0 77 1650.0 78 1083.0 79 516.0 80 516.0 81 516.0 82 340.0 83 164.0 84 164.0 85 164.0 86 100.5 87 37.0 88 37.0 89 37.0 90 22.0 91 7.0 92 7.0 93 7.0 94 5.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.04999117437994 2 36.17263866957355 3 5.520783142588626 4 13.526336127317917 5 13.226265045296898 6 14.484750759718915 7 9.45653262855589 8 7.451447163159476 9 0.10972392506332979 10 1.4042277105387444 11 5.541177480834093 12 5.708625731691609 13 15.961730203180085 14 1.5632081367446342 15 2.90839960499387 16 2.8522257259668824 17 2.8766750788342548 18 1.4671997023142207 19 1.4110258232872332 20 0.30114446824446495 21 4.342920660060969 22 1.5605843037539895 23 0.11246702318991303 24 8.997004059785226 25 0.11497159104461947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 838468.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.3186683050509 #Duplication Level Percentage of deduplicated Percentage of total 1 96.75073574537917 89.31899081547292 2 2.3531834344774856 4.344855218969349 3 0.4202800356664013 1.163990796237644 4 0.15608257893719182 0.5763734333239815 5 0.09342864410936365 0.43126040028614954 6 0.04572658377482439 0.2532850392138681 7 0.03721337344473661 0.240484235568964 8 0.022038418139324552 0.16276459313378563 9 0.016561313127368967 0.13760265359714946 >10 0.09132534506861645 1.5200026693428041 >50 0.006513755804983823 0.41208344989694357 >100 0.006113375363217972 0.9461930797219116 >500 7.973967073060083E-4 0.4921136152345251 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 936 0.11163216723834421 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1926513593840194E-4 2 0.0 0.0 0.0 0.0 1.1926513593840194E-4 3 0.0 0.0 0.0 0.0 1.1926513593840194E-4 4 0.0 0.0 0.0 0.0 1.1926513593840194E-4 5 0.0 0.0 0.0 0.0 1.1926513593840194E-4 6 0.0 0.0 0.0 0.0 1.1926513593840194E-4 7 0.0 0.0 0.0 0.0 1.1926513593840194E-4 8 0.0 0.0 0.0 0.0 1.1926513593840194E-4 9 0.0 0.0 0.0 0.0 1.1926513593840194E-4 10 0.0 0.0 0.0 0.0 1.1926513593840194E-4 11 0.0 0.0 0.0 0.0 1.1926513593840194E-4 12 0.0 0.0 0.0 0.0 1.1926513593840194E-4 13 0.0 0.0 0.0 0.0 1.1926513593840194E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTAGA 70 2.108718E-7 19.047968 1 GTCTTAG 90 1.6325976E-7 16.296595 1 CTCCTAG 75 8.558287E-6 16.000294 1 AGTACTA 40 0.0028651264 15.619457 5 GTCCTAA 330 0.0 15.353817 1 GTATAGA 80 1.5774345E-5 15.000274 1 TCTAGTA 55 0.003908929 14.906359 2 TAGGACC 285 0.0 14.655701 4 AGAGTAG 60 1.8798102E-4 13.883963 5 TAAAGGT 70 3.5394944E-5 13.769917 4 TATAGGA 110 1.1998691E-6 13.664162 2 CTTATAC 65 2.464309E-4 13.447305 3 ATATTAC 65 2.464309E-4 13.447305 3 TGTAGGA 625 0.0 13.336223 2 GTCCAAA 60 0.008187159 13.333577 1 GTGTAGG 95 7.9459365E-5 12.631811 1 GCCTTAG 75 0.0024675773 12.444673 1 TCCAACG 90 1.4767829E-7 12.325119 18 CTGTAGG 675 0.0 12.247139 1 TGTTATA 90 5.8143405E-4 12.145923 2 >>END_MODULE