FastQCFastQC Report
Thu 26 May 2016
SRR1926638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838468
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA19340.23065877290486936No Hit
GTATCAACGCAGAGTACTTTTTTTT19310.23030097749705417No Hit
GTCCTACAGTGGACATTTCTAAATT17130.20430117786248253No Hit
CTTTAGGACGTGAAATATGGCGAGG15740.18772332396704466No Hit
GTCCTAAAGTGTGTATTTCTCATTT14740.17579681037320447No Hit
GGTATCAACGCAGAGTACTTTTTTT11620.13858608796042307No Hit
GTCCTACAGTGTGCATTTCTCATTT10960.1307145889884885No Hit
CTGTAGGACCTGGAATATGGCGAGA9810.1169990983555723No Hit
TATCAACGCAGAGTACTTTTTTTTT9090.10841200856800738No Hit
CTGAAGGACCTGGAATATGGCGAGA8420.10042124446013445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG653.4635614E-614.57587918
AGCGTAC400.00534655314.22166210
GACGTGA4550.012.7329777
CCTACAC1301.8371793E-1012.4780683
TAGGACC3750.012.4645384
AGGACGT9500.012.4504025
GTATTAG550.002977303212.1385961
ACACCGT550.002977303212.1385966
GGACGTG9500.012.0473296
GGCGAGG4850.011.91402519
TTAGGAC5850.011.9071763
ACGTGAA5200.011.689298
CACGTTT658.200741E-411.66070418
TAGGACG10450.011.5017954
TAATACT1001.8327992E-611.4470234
AGGACCT7400.011.3432175
TGTAGGA11400.011.2174432
GACGTGG5550.011.1232557
CTGTAGG11150.011.034381
TTTAGGA6750.011.0276972
Produced by FastQC (version 0.11.5)