FastQCFastQC Report
Thu 26 May 2016
SRR1926637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755786
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG300.001592260817.05562619
TAGGACC1600.016.086724
GTATTAG1105.9499143E-915.7356961
TATGCTG807.1978684E-714.29877955
TTAGGCT703.493311E-513.7886164
TATTAGG1006.887516E-613.6357922
GTCCTAG700.001483979613.3148191
CTTGGAC500.00917085113.1068583
TGTAGGA3350.012.6181962
ATAGGAC1056.4261803E-712.4827223
GTATAAA750.002493105412.4271651
GTCTTAG750.002493105412.4271651
CTAGACT953.5134544E-612.4178194
TTAGACA705.3895917E-412.2565484
GTAGCCC1451.6116246E-912.0522823
TATGGAA800.003382296511.9313182
TAGCCCT1452.1482265E-911.8339084
TCTATAC650.003764647811.7625663
CCAGTAC953.9769224E-511.4972453
AGCTAAG1052.0459268E-411.4127021
Produced by FastQC (version 0.11.5)