FastQCFastQC Report
Thu 26 May 2016
SRR1926637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755786
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15440.20429063253354787No Hit
GGTATCAACGCAGAGTACTTTTTTT9500.12569695654590055No Hit
TATCAACGCAGAGTACTTTTTTTTT7850.1038653798826652No Hit
GTACATGGGAAGCAGTGGTATCAAC7630.10095450299423382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT400.005341625314.2233810
ATGCCGG602.6316993E-514.21114519
TAGGCAG1051.2896635E-913.6227545
TAGGACC2450.012.8443124
TGTAGGA4900.012.4584092
GGCGAGG2150.012.33835819
GTAGGAC4650.011.8950683
CTATTGG657.7781297E-411.7341821
CTGTAGG5100.011.5903821
AGGACGT3800.011.5434925
CCTACCC751.9984978E-411.4438763
GGACGTG3750.011.4431136
GGCTAGG1001.8439841E-611.4408281
TAGGACG3850.011.3935774
CTTTAGG3300.011.2674831
TAGCACC855.113667E-511.21873954
GCTTAGA855.1240888E-511.2164981
GTATAAA1451.1423253E-911.1778211
AATGCCG855.4604046E-511.14673418
TCTTAGG600.00569687811.1282152
Produced by FastQC (version 0.11.5)