FastQCFastQC Report
Thu 26 May 2016
SRR1926636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839566
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA1700.016.3844971
CCCTAAG654.7245776E-516.324481
TGTAGGA3000.014.4787762
GTCCTAC3350.014.2534631
TAGGACC1053.8453436E-914.2387634
GCTTAAT751.6010222E-414.1478831
ATAAGGC703.1049505E-513.96285253
GTATAAG955.39909E-613.9617261
GTGTAGC1356.2409526E-913.7548871
CTTTAGG1851.8189894E-1213.6221161
TAGGACG2550.013.4011894
CTAAGGC652.5940203E-413.366153
GTAGGAC2850.013.3368383
CTGTAGG3050.013.0462041
TCCAATG853.7986596E-412.7753912
AAGGCTC653.8835048E-412.743175
GCCATAT1152.762099E-612.686961
AGGACGT2450.012.6781535
GACAGAT654.6389352E-412.4774587
TCCTACA3700.012.4732692
Produced by FastQC (version 0.11.5)