##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926636_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 839566 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.13065798281493 27.0 7.0 31.0 2.0 31.0 2 16.436209898923966 16.0 2.0 30.0 2.0 31.0 3 19.805784178968658 23.0 15.0 31.0 2.0 31.0 4 21.886809375320105 28.0 2.0 35.0 2.0 35.0 5 22.48640011625054 28.0 2.0 35.0 2.0 35.0 6 23.420438655209953 30.0 2.0 35.0 2.0 37.0 7 23.299109301710647 30.0 7.0 35.0 2.0 37.0 8 23.369031142280654 30.0 8.0 35.0 2.0 37.0 9 26.060059602223053 31.0 17.0 37.0 2.0 39.0 10 26.767690687807747 32.0 23.0 37.0 2.0 39.0 11 23.932223315379613 30.0 7.0 37.0 2.0 39.0 12 25.057253390442206 31.0 10.0 37.0 2.0 39.0 13 21.77801506969077 27.0 2.0 35.0 2.0 39.0 14 25.026672113925528 31.0 16.0 38.0 2.0 40.0 15 26.085913436227766 31.0 17.0 38.0 2.0 40.0 16 26.450797197599712 31.0 17.0 38.0 2.0 40.0 17 25.74963493042834 31.0 10.0 38.0 2.0 40.0 18 25.789769952570733 31.0 11.0 38.0 2.0 40.0 19 26.201517212464534 31.0 13.0 39.0 2.0 40.0 20 26.454682538359105 31.0 11.0 39.0 2.0 40.0 21 24.342834273898657 30.0 2.0 37.0 2.0 40.0 22 25.251817010217184 31.0 2.0 38.0 2.0 40.0 23 25.23753343989633 31.0 2.0 38.0 2.0 40.0 24 24.335885445575453 31.0 2.0 38.0 2.0 40.0 25 24.11935452364674 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.904829484023928 1101 2 10.259414590270127 1101 3 6.898324603541429 1101 4 8.749724713900829 1101 5 8.343221600206707 1101 6 8.085615535401363 1101 7 7.571347042058189 1101 8 6.285839877206826 1101 9 6.015332340680473 1101 10 5.824679763625369 1101 11 8.675813437693002 1101 12 6.400156388573244 1101 13 10.844164950958074 1101 14 8.222813334448798 1101 15 7.35952995766737 1101 16 6.978497932707153 1101 17 2.8187121629121386 1101 18 5.137922282334486 1101 19 5.8125403611274 1101 20 4.112333739460016 1101 21 1.0708913528714028 1101 22 4.921134217033817 1101 23 5.994393653089812 1101 24 7.184127775790344 1101 25 7.797871905996768 1102 1 5.383956791126202 1102 2 10.378564956442142 1102 3 6.448672743584293 1102 4 7.824088847758183 1102 5 7.813920325537396 1102 6 6.883606882785976 1102 7 6.707271242196708 1102 8 6.884618893999782 1102 9 5.984602851709347 1102 10 5.299508084206547 1102 11 8.201079375157569 1102 12 5.3361073634609895 1102 13 9.740184665916278 1102 14 7.115607784972202 1102 15 5.36859922093829 1102 16 5.657368967209699 1102 17 6.559349538549576 1102 18 6.593601192984689 1102 19 5.321309774528164 1102 20 5.655590669133954 1102 21 4.511469851381719 1102 22 5.473248670890406 1102 23 6.044111357073103 1102 24 7.345836332645021 1102 25 7.192466138770428 1103 1 4.862175298671755 1103 2 9.204875057979377 1103 3 6.039013214520921 1103 4 7.843816447267095 1103 5 6.9002669255079 1103 6 6.73077323190623 1103 7 7.042246868055351 1103 8 6.559285630065524 1103 9 5.48574268581817 1103 10 4.777488260726738 1103 11 5.652376521440313 1103 12 4.4073044198445395 1103 13 8.25992390803275 1103 14 6.477980064425829 1103 15 5.5195928832506205 1103 16 5.360226337048772 1103 17 3.2438083924330243 1103 18 1.5561389344375307 1103 19 3.9764041458524666 1103 20 4.378148244111202 1103 21 6.604879176699459 1103 22 2.195164304446866 1103 23 4.515084510133214 1103 24 5.5987941256814615 1103 25 1.872653753684105 1104 1 5.573517411755773 1104 2 10.160997004546802 1104 3 6.815439628292918 1104 4 7.327667354148211 1104 5 7.289630090476429 1104 6 7.045074851384324 1104 7 7.023549902525261 1104 8 6.968901364419661 1104 9 5.9561941968431285 1104 10 4.770696300009394 1104 11 8.085231832982476 1104 12 4.89043186225792 1104 13 9.148226938066948 1104 14 6.804016800984517 1104 15 6.183592652593646 1104 16 5.789430614848481 1104 17 6.103531249208352 1104 18 3.6123979444755463 1104 19 4.522899268788198 1104 20 4.042434116815169 1104 21 2.9798036587193018 1104 22 5.195364355013972 1104 23 5.54686238746806 1104 24 6.49136963257234 1104 25 5.4938254023935365 1105 1 3.1943923113978094 1105 2 9.062879076733564 1105 3 6.008202765760036 1105 4 5.524325489464012 1105 5 1.6845937841817111 1105 6 4.298757156889483 1105 7 5.622287055597212 1105 8 2.770940017205511 1105 9 3.8702035029648556 1105 10 3.447950417458152 1105 11 6.675149461828795 1105 12 3.7884162751693893 1105 13 4.9946861317434 1105 14 3.3400028373662174 1105 15 3.9911267077650194 1105 16 4.3173596257292814 1105 17 4.927666396451777 1105 18 2.64723069599145 1105 19 2.3454597386187075 1105 20 1.3868555448479754 1105 21 2.311510121106693 1105 22 3.8910785710242273 1105 23 1.2143477385324069 1105 24 4.003348713759159 1105 25 4.686451476390065 1106 1 6.040908866461564 1106 2 10.633266297165205 1106 3 7.324942853095365 1106 4 8.637818133115239 1106 5 8.172790223964231 1106 6 7.007006620443512 1106 7 6.915689338487429 1106 8 6.810332727136448 1106 9 5.633104438400718 1106 10 5.134077933332932 1106 11 7.458890470784425 1106 12 5.090508807946367 1106 13 4.434980175837772 1106 14 5.380656435795093 1106 15 5.4757035581320075 1106 16 5.576047252408351 1106 17 5.9335288284451195 1106 18 5.992212237353314 1106 19 5.6751868900425 1106 20 5.149680844462015 1106 21 5.529518354481237 1106 22 5.87373829376866 1106 23 6.3338680762616555 1106 24 7.096596752037005 1106 25 6.245207831110704 1107 1 3.8671985188821267 1107 2 -13.633704982208164 1107 3 -5.39409811565111 1107 4 1.5245035073681628 1107 5 4.4214027687453346 1107 6 4.856751162136561 1107 7 4.790545128152861 1107 8 5.310086376850631 1107 9 3.5670630446132563 1107 10 3.0086756623996607 1107 11 5.67181518648405 1107 12 2.977336440815048 1107 13 4.381403325825193 1107 14 4.001598812381406 1107 15 3.922355276078278 1107 16 3.59189260688386 1107 17 4.093571072314553 1107 18 -0.8838683642965393 1107 19 1.7252996560184002 1107 20 2.2547110744178624 1107 21 -1.1141502696871122 1107 22 1.876447279482079 1107 23 -0.991508605469388 1107 24 2.3328362669698244 1107 25 3.077407721814449 1108 1 5.213335025341124 1108 2 10.401801796358653 1108 3 0.295596875651821 1108 4 5.745705054181133 1108 5 7.202038437669021 1108 6 7.084411060602832 1108 7 7.2093749808710434 1108 8 7.14103121533822 1108 9 5.675632928945447 1108 10 4.658727458757401 1108 11 6.936553984851447 1108 12 2.4610110438704993 1108 13 3.379048360988808 1108 14 4.825440150378913 1108 15 4.806488432396041 1108 16 4.619578242670745 1108 17 -0.2553309092446554 1108 18 3.4941734116643737 1108 19 4.623752628571694 1108 20 4.841711582729943 1108 21 5.053996793362888 1108 22 2.473974780881548 1108 23 4.841334360413647 1108 24 5.462850057884118 1108 25 5.898942995884742 1109 1 3.0782896820429926 1109 2 -13.633704982208164 1109 3 -5.660356826170364 1109 4 1.5116008338287088 1109 5 0.4559982212296241 1109 6 2.0806445907265 1109 7 3.2014410276151892 1109 8 3.502156912391399 1109 9 3.159601380152175 1109 10 2.1784625380879454 1109 11 0.38534159335437224 1109 12 1.1562484240834472 1109 13 2.278429487459114 1109 14 3.204672596147681 1109 15 3.017157189847609 1109 16 2.9837470567677187 1109 17 3.556836973350098 1109 18 3.62142596565284 1109 19 2.1726668350594807 1109 20 1.0836994048669695 1109 21 0.333752265039287 1109 22 2.498039187606345 1109 23 3.025361345205706 1109 24 4.145288723498201 1109 25 4.333318844441905 1110 1 2.734499982622239 1110 2 8.620130443175379 1110 3 5.996481866463366 1110 4 7.084308805375272 1110 5 7.917685730699212 1110 6 3.485177560427708 1110 7 5.972910280110305 1110 8 6.778349303311476 1110 9 4.974071296975211 1110 10 4.512129977861374 1110 11 2.68166195481092 1110 12 5.2523768685848715 1110 13 9.572176063839343 1110 14 7.113281858665989 1110 15 6.0232954244447185 1110 16 5.72396375975875 1110 17 6.213957325482745 1110 18 6.326911138708322 1110 19 5.214887593947296 1110 20 5.563387870036372 1110 21 3.317118814768701 1110 22 5.488427800015682 1110 23 5.944648416451962 1110 24 7.079368745875563 1110 25 7.338476126991058 1111 1 4.789934375758399 1111 2 9.1120805875828 1111 3 6.986772571628649 1111 4 7.673646731908526 1111 5 7.879189467611553 1111 6 7.102319205722452 1111 7 6.86277458133544 1111 8 7.124943573473264 1111 9 5.650281267529451 1111 10 4.862711322359832 1111 11 6.682914175468845 1111 12 6.020083588443878 1111 13 7.750805834433272 1111 14 5.7979136361986185 1111 15 5.236407110489534 1111 16 5.222072967695091 1111 17 6.432899854996688 1111 18 5.909932474893125 1111 19 5.1569572236229035 1111 20 4.746530431337817 1111 21 7.284211227054474 1111 22 6.673288108993958 1111 23 6.718176575193851 1111 24 6.486783885942767 1111 25 7.008440041593651 1112 1 -1.530641084673988 1112 2 7.8662950177918365 1112 3 6.099987706283223 1112 4 -0.034120910308480745 1112 5 4.5014729259494395 1112 6 3.402013694848751 1112 7 6.0042160388973045 1112 8 6.19846960593803 1112 9 4.9528274426161865 1112 10 3.677735036181655 1112 11 0.08006701001455596 1112 12 4.3243324875122795 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0.36098089036737235 2204 6 3.452152061308425 2204 7 4.373392035098721 2204 8 4.514709302644686 2204 9 2.9867958520325253 2204 10 2.5776864409907247 2204 11 4.2317433586238415 2204 12 4.3503476076648155 2204 13 2.292280305172479 2204 14 3.681366563084019 2204 15 3.644595440558785 2204 16 3.5398716762100975 2204 17 -1.330859224218031 2204 18 2.226803896825089 2204 19 2.968775806786315 2204 20 3.115197368444413 2204 21 4.510268114362514 2204 22 4.218753359701623 2204 23 2.943012005332143 2204 24 4.70207735356475 2204 25 4.785613657760589 2205 1 6.186797666394359 2205 2 10.514029751869314 2205 3 7.380719465491197 2205 4 7.879328636124793 2205 5 8.171950188922768 2205 6 7.797084435118066 2205 7 7.923712530312002 2205 8 7.484031401424005 2205 9 6.274953355526975 2205 10 5.742585173920681 2205 11 8.054962192633393 2205 12 7.179954110521848 2205 13 10.366336455271142 2205 14 8.045029006765194 2205 15 6.795951059884935 2205 16 6.365245601825212 2205 17 7.1530218415822375 2205 18 7.028215453487476 2205 19 5.569128608754195 2205 20 6.096511769409169 2205 21 -0.7637014484019673 2205 22 4.176666073041741 2205 23 5.757660611607417 2205 24 7.503806309921419 2205 25 7.801010158864948 2206 1 4.4510008554643825 2206 2 8.854374487990514 2206 3 6.304843601802851 2206 4 2.3157468243468884 2206 5 6.032014196436695 2206 6 6.25633312953002 2206 7 6.40235784954513 2206 8 6.394066927333569 2206 9 5.105200819359684 2206 10 4.145530737095488 2206 11 3.72960269390963 2206 12 4.646061001330768 2206 13 7.979352765881519 2206 14 0.39606978111696733 2206 15 2.631274771798946 2206 16 3.5629405486233168 2206 17 4.691610719407027 2206 18 -0.5670722541999496 2206 19 1.2579986065365425 2206 20 2.561639414828413 2206 21 3.5261612746133437 2206 22 -1.8728475655158299 2206 23 -2.7703561555782983 2206 24 1.1721643345590955 2206 25 2.72163997192996 2207 1 1.2514552536791825 2207 2 -13.633704982208164 2207 3 -6.1571777054243295 2207 4 -4.420286504187519 2207 5 1.0870593663186412 2207 6 1.7112118366996114 2207 7 2.590595684270152 2207 8 2.9137887096543302 2207 9 0.4638829745569204 2207 10 0.2242343074957205 2207 11 -1.4710586579475553 2207 12 -2.483199544307009 2207 13 0.5235472663134217 2207 14 -5.2430327599138415 2207 15 -1.657734190193569 2207 16 -0.673503987800526 2207 17 0.6182564507632016 2207 18 0.6309481892207209 2207 19 0.06818162554028362 2207 20 -0.16271273029108002 2207 21 -3.7305530213843525 2207 22 -3.9747263446692997 2207 23 -1.1089035209684504 2207 24 0.7006735484951747 2207 25 1.2109548653938234 2208 1 4.917308486925965 2208 2 6.603906199494505 2208 3 6.51971859864727 2208 4 8.286592604701493 2208 5 3.4508315681306954 2208 6 6.278917004922363 2208 7 6.7276185006891325 2208 8 6.805122406687641 2208 9 5.166881945608246 2208 10 4.733063239598916 2208 11 6.874087155819641 2208 12 6.152102890641338 2208 13 8.809529394372642 2208 14 3.0952367072426696 2208 15 4.512485169234086 2208 16 5.096528572555254 2208 17 5.869142969463827 2208 18 0.7688618404002163 2208 19 3.1684894469313676 2208 20 4.115303677642185 2208 21 6.800596846026288 2208 22 5.905287190494505 2208 23 1.9689581993784913 2208 24 3.465185868256512 2208 25 4.598063139798551 2209 1 3.004270356225046 2209 2 5.165886019836828 2209 3 5.268713643379794 2209 4 1.0551726923389602 2209 5 5.296537912449459 2209 6 5.601398293828922 2209 7 6.369166266054922 2209 8 4.740106283229505 2209 9 4.797145455662992 2209 10 3.8591536193122025 2209 11 0.392626750754026 2209 12 3.680347308927928 2209 13 2.5697688513286856 2209 14 3.847714124440504 2209 15 4.389730781947151 2209 16 3.7937016188797728 2209 17 1.7202220675202646 2209 18 3.609279447926749 2209 19 4.286544948352294 2209 20 4.271814254682692 2209 21 6.461026770263789 2209 22 5.0812922973389405 2209 23 0.9541318069708531 2209 24 -2.322193660538346 2209 25 0.6280489608563187 2210 1 -2.7134332423020844 2210 2 -13.633704982208164 2210 3 -7.586042967116628 2210 4 -2.579985652975573 2210 5 -0.7020392271248106 2210 6 -1.488417538023505 2210 7 -1.4565690521389385 2210 8 -20.527787377302193 2210 9 -12.90484863261984 2210 10 -7.16152846068351 2210 11 -9.89367925949176 2210 12 -4.694338277615028 2210 13 -5.28182502025072 2210 14 -4.062200652768727 2210 15 -5.70346211089479 2210 16 -5.354249075136771 2210 17 -4.877203686253441 2210 18 -4.867192751226085 2210 19 -10.079238830443513 2210 20 -7.21028092217767 2210 21 -4.994794604195569 2210 22 -10.023259665345206 2210 23 -7.894589553498037 2210 24 -11.036957772307307 2210 25 -8.630828289153357 2211 1 0.031218452436945654 2211 2 4.742723589220409 2211 3 0.6670148411595207 2211 4 1.3714138063714856 2211 5 0.8031967886788891 2211 6 -0.028780399325231798 2211 7 -20.442013011382024 2211 8 -13.032073091587907 2211 9 -8.938900410536245 2211 10 -7.169663208032233 2211 11 -9.369214713848175 2211 12 -4.84356056915173 2211 13 -6.991437205736268 2211 14 -4.883882915193347 2211 15 -5.3032427305662395 2211 16 -11.341628987800526 2211 17 -7.6341697914728215 2211 18 -5.880728829412817 2211 19 -8.663903001788906 2211 20 -6.605072978738285 2211 21 -4.46006389094957 2211 22 -5.931310195600975 2211 23 -8.254183024074042 2211 24 -21.590087321070044 2211 25 -21.36445274330183 2212 1 -0.4799657870523788 2212 2 -13.633704982208164 2212 3 -7.300140122520867 2212 4 -19.120014765057086 2212 5 -19.573946068463968 2212 6 -20.469494685039518 2212 7 -9.493630885033795 2212 8 -9.03626195357338 2212 9 -4.6088574873577315 2212 10 -5.0788113480278305 2212 11 -5.055556338325392 2212 12 -2.113763078506782 2212 13 -5.628312618459139 2212 14 -3.137968321312652 2212 15 -3.1012143352327612 2212 16 -3.2832160448113115 2212 17 -3.610011746128336 2212 18 -2.60358487167564 2212 19 -2.9470749146218367 2212 20 -3.1225757302240886 2212 21 -1.0258882360959518 2212 22 -2.2500412103489396 2212 23 -2.418568892442959 2212 24 -1.5153569666786737 2212 25 -2.0092909559366525 2213 1 -18.42449583327734 2213 2 -13.633704982208164 2213 3 -8.859632829527655 2213 4 -6.547590287907589 2213 5 -19.573946068463968 2213 6 -11.066706149020927 2213 7 -20.442013011382024 2213 8 -11.021203333924966 2213 9 -9.18364811442028 2213 10 -8.259387674116109 2213 11 -7.332747966033635 2213 12 -6.115251395753479 2213 13 -18.83786577716484 2213 14 -12.622932378510818 2213 15 -7.892090246334746 2213 16 -7.162938050542586 2213 17 -5.695666969739946 2213 18 -5.752883858736709 2213 19 -6.602715660857182 2213 20 -6.481125319116728 2213 21 -4.681194797223778 2213 22 -5.71051845055284 2213 23 -5.416791743781907 2213 24 -4.488615593726898 2213 25 -5.2955139361755705 2214 1 -18.42449583327734 2214 2 -13.633704982208164 2214 3 -12.25897573688988 2214 4 -19.120014765057086 2214 5 -19.573946068463968 2214 6 -20.469494685039518 2214 7 -16.363581638833004 2214 8 -15.020657252525009 2214 9 -14.169161424032554 2214 10 -14.102981262526756 2214 11 -14.744806768851994 2214 12 -12.580680508036373 2214 13 -18.83786577716484 2214 14 -16.75569091111899 2214 15 -23.383957016280526 2214 16 -18.200826848763093 2214 17 -23.098455505758537 2214 18 -17.73105095826436 2214 19 -15.219938143118167 2214 20 -13.938491618921631 2214 21 -11.474466742999482 2214 22 -12.505426569423232 2214 23 -12.426106884527313 2214 24 -11.299534736399812 2214 25 -11.055183581091491 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 154984.0 3 10.0 4 683.0 5 2875.0 6 3915.0 7 3813.0 8 3308.0 9 3218.0 10 3029.0 11 2714.0 12 2731.0 13 2862.0 14 4014.0 15 8465.0 16 12059.0 17 12881.0 18 13029.0 19 15206.0 20 18183.0 21 21360.0 22 22800.0 23 24999.0 24 26244.0 25 26342.0 26 22321.0 27 17895.0 28 19387.0 29 22102.0 30 21549.0 31 25076.0 32 31790.0 33 39966.0 34 48094.0 35 54943.0 36 53501.0 37 55460.0 38 37758.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02613386368435 18.86800934471227 12.303553306407121 25.80230348519626 2 13.724399997054295 20.126517957743886 42.21284915789706 23.936232887304758 3 18.518946468111082 25.145783121937864 29.05602367193868 27.279246738012375 4 11.653453326719813 17.06480245759902 38.99999725959075 32.28174695609042 5 12.238810043668122 37.704557860262014 36.47748362445415 13.57914847161572 6 31.66955004141826 37.94604147910901 18.041490172324856 12.342918307147865 7 27.555347995123203 31.240548587455326 23.063408839981374 18.1406945774401 8 24.38464736893438 37.67767585353709 20.286232014250004 17.651444763278526 9 25.95623104798177 15.806832802507898 20.14318573961611 38.09375040989422 10 13.364510231050982 30.231654407105918 35.44559832489546 20.95823703694764 11 33.09693710897258 22.77167768034373 24.74873740546245 19.382647805221236 12 22.664360079104554 25.274357778384037 32.251952783683244 19.80932935882817 13 27.719679955081418 21.653986524424482 25.55769230769231 25.068641212801797 14 19.93703166545806 22.477157360406093 28.584481508339376 29.00132946579647 15 22.316268988446208 31.288918278503335 23.866326236966014 22.528486496084447 16 21.471159807772896 29.08672520395335 27.76547018935987 21.676644798913884 17 21.020945292511843 28.69921286831764 28.871573896118203 21.40826794305231 18 20.80726066071433 28.310514964969435 30.295097170264718 20.587127204051516 19 22.11853196481505 27.79589541070032 27.997938532544147 22.087634091940483 20 22.31514741737376 28.18114161409511 28.712252706623836 20.791458261907295 21 21.750820371504894 26.83551148272073 27.27911977796022 24.134548367814155 22 21.70702108937602 28.426540123964145 28.598417088099637 21.2680216985602 23 21.575368192859006 28.213273425603642 28.412528633247597 21.798829748289755 24 21.40854798876602 27.979382652496042 28.901168359800085 21.710900998937856 25 21.883559724443206 27.85141302157304 28.500714887913446 21.76431236607031 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 932.0 1 932.0 2 1793.5 3 2655.0 4 2655.0 5 2655.0 6 3804.0 7 4953.0 8 4953.0 9 4953.0 10 5943.0 11 6933.0 12 6933.0 13 6933.0 14 8786.5 15 10640.0 16 10640.0 17 10640.0 18 15256.5 19 19873.0 20 19873.0 21 19873.0 22 27365.0 23 34857.0 24 34857.0 25 34857.0 26 44791.5 27 54726.0 28 54726.0 29 54726.0 30 65062.0 31 75398.0 32 75398.0 33 75398.0 34 83891.5 35 92385.0 36 92385.0 37 92385.0 38 97265.0 39 102145.0 40 102145.0 41 102145.0 42 103253.5 43 104362.0 44 104362.0 45 104362.0 46 102517.5 47 100673.0 48 100673.0 49 100673.0 50 92755.0 51 84837.0 52 84837.0 53 84837.0 54 73105.0 55 61373.0 56 61373.0 57 61373.0 58 50753.0 59 40133.0 60 40133.0 61 40133.0 62 31602.5 63 23072.0 64 23072.0 65 23072.0 66 17378.5 67 11685.0 68 11685.0 69 11685.0 70 8360.5 71 5036.0 72 5036.0 73 5036.0 74 3493.0 75 1950.0 76 1950.0 77 1950.0 78 1315.5 79 681.0 80 681.0 81 681.0 82 434.5 83 188.0 84 188.0 85 188.0 86 125.0 87 62.0 88 62.0 89 62.0 90 38.5 91 15.0 92 15.0 93 15.0 94 8.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.561370994061216 2 35.30419288060736 3 5.163262923939273 4 13.0719919577496 5 12.716808446268669 6 13.870857085684746 7 8.948671099115495 8 7.186808422446836 9 0.11136706345897761 10 1.3055554893838008 11 5.191491794570052 12 5.260217779185913 13 15.146635285373634 14 1.449082025713285 15 2.7336743031518664 16 2.792871555065355 17 2.7009192844874614 18 1.3616559031690183 19 1.313774021339597 20 0.2921747664864942 21 3.9572826912952643 22 1.4567050118751832 23 0.11231993672921486 24 8.605755830988867 25 0.11589321149260451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 839566.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.8047549778557 #Duplication Level Percentage of deduplicated Percentage of total 1 97.26108084136055 92.208129380466 2 2.154826962442091 4.08575684387999 3 0.29367658658302537 0.8352581050121023 4 0.10566958834738117 0.4007191772753739 5 0.04975494589849032 0.23585027274214196 6 0.0327225046988308 0.18613494241406311 7 0.021626301113994967 0.14351933247327342 8 0.014900808271094794 0.11301339816905179 9 0.009932234004660812 0.08474607100751351 >10 0.04890414381753095 0.8575013410748382 >50 0.004475918047964932 0.30453827666818684 >100 0.002429165414348731 0.5448328588174888 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.191091587796552E-4 0.0 0.0 0.0 6 0.0 1.191091587796552E-4 0.0 0.0 0.0 7 0.0 1.191091587796552E-4 0.0 0.0 0.0 8 0.0 1.191091587796552E-4 0.0 1.191091587796552E-4 0.0 9 0.0 1.191091587796552E-4 0.0 1.191091587796552E-4 0.0 10 0.0 1.191091587796552E-4 0.0 2.382183175593104E-4 0.0 11 0.0 1.191091587796552E-4 0.0 2.382183175593104E-4 0.0 12 0.0 1.191091587796552E-4 0.0 2.382183175593104E-4 0.0 13 0.0 1.191091587796552E-4 0.0 2.382183175593104E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 170 0.0 16.384497 1 CCCTAAG 65 4.7245776E-5 16.32448 1 TGTAGGA 300 0.0 14.478776 2 GTCCTAC 335 0.0 14.253463 1 TAGGACC 105 3.8453436E-9 14.238763 4 GCTTAAT 75 1.6010222E-4 14.147883 1 ATAAGGC 70 3.1049505E-5 13.9628525 3 GTATAAG 95 5.39909E-6 13.961726 1 GTGTAGC 135 6.2409526E-9 13.754887 1 CTTTAGG 185 1.8189894E-12 13.622116 1 TAGGACG 255 0.0 13.401189 4 CTAAGGC 65 2.5940203E-4 13.36615 3 GTAGGAC 285 0.0 13.336838 3 CTGTAGG 305 0.0 13.046204 1 TCCAATG 85 3.7986596E-4 12.775391 2 AAGGCTC 65 3.8835048E-4 12.74317 5 GCCATAT 115 2.762099E-6 12.68696 1 AGGACGT 245 0.0 12.678153 5 GACAGAT 65 4.6389352E-4 12.477458 7 TCCTACA 370 0.0 12.473269 2 >>END_MODULE