FastQCFastQC Report
Thu 26 May 2016
SRR1926636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839566
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14060.16746747724419522No Hit
GTACATGGGAAGCAGTGGTATCAAC10220.12172956027280762No Hit
CCCATGTACTCTGCGTTGATACCAC9560.11386835579335038No Hit
CTGTAGGACGTGGAATATGGCAAGA8880.10576893299633382No Hit
GTCCTACAGTGGACATTTCTAAATT8590.10231476739172382No Hit
GGTATCAACGCAGAGTACTTTTTTT8540.10171922159782554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG653.458461E-614.57795619
GTTGCAC500.001458546413.3478183
AAAGGCG500.001459205413.3470195
GGACGTG3800.012.544196
GCAGGAC1103.6185156E-812.134383
CTTACAC1353.4924597E-1012.0059743
GACGTGG1850.011.8108117
GACGTGA2500.011.787
TAGGACG4050.011.7698584
TTAGGAC3000.011.7587923
GTAGGAC4000.011.4409873
AGGACGT4250.011.4403025
GATATAC1751.8189894E-1211.437561
ACTGTTC2500.011.4006818
TTTAGGA3700.011.3385952
TTACACC1104.7359754E-711.2669644
CAAGACA1451.1441443E-911.1773064
GGTATAC600.0057297711.119851
CTTTAGG3700.011.0769171
GTCCTAC4350.010.9555191
Produced by FastQC (version 0.11.5)