FastQCFastQC Report
Thu 26 May 2016
SRR1926635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751241
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAAGA350.00528668319.4347172
GTCCTAC2450.016.7943951
GTAGGAC2300.016.118073
TCCTACA2650.015.9144672
TAGGACC959.0221874E-1015.7765944
CTGTAGG2150.015.433711
GGACGTG1500.015.2257986
TGTAGGA2450.014.99249652
TAGGACG1750.014.6818924
ACAGCGT400.00446181114.6135088
CTATTAA550.00474033814.4796271
TCTATAC1156.7484507E-1014.2218273
CTAACAC652.5070258E-413.4195713
CTTAGAC652.5070258E-413.4195713
GTATTTA700.001519583313.2729911
GTTTTAT600.0084355613.2729911
GTCTTAC600.0084355613.2729911
GTCCTAA1051.50382675E-512.6409441
CTATACC600.002402093312.4898054
GCCATAT750.002552654612.3881261
Produced by FastQC (version 0.11.5)