FastQCFastQC Report
Thu 26 May 2016
SRR1926635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751241
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17940.23880485756235348No Hit
GGTATCAACGCAGAGTACTTTTTTT11130.14815485310306548No Hit
TATCAACGCAGAGTACTTTTTTTTT8880.11820441110109806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG653.2521657E-614.6641121
TAGGACC1700.014.5827344
GGTTAGA707.0054266E-613.6166751
CTTATAC500.001456006113.3505993
ACCGTGC500.001493925513.30514058
GTAGGAC2800.013.2824843
GGACAGT2000.012.8712056
GACAGTC905.371585E-712.6707177
GTATTAG550.00299039412.1312191
CGTGAAA1406.730261E-1011.5401739
TTAGGAC2000.011.4433713
CTAGGAC751.9992873E-411.4433713
CTACACT1501.6370905E-1011.4418384
ATAAGAC600.005707184311.12553
TAACCTG600.00571288111.1240095
GATATAC600.005727144411.1202841
GTAGAAC2150.011.0885373
ATTAGAT600.00595658111.061769512
GTTTTAA951.3082945E-511.0366741
CTAACAC700.001443370510.8984493
Produced by FastQC (version 0.11.5)